{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:26:51Z","timestamp":1787027211379,"version":"build-2736575974"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell'Istruzione, dell'Universit\u00e1 e della Ricerca","doi-asserted-by":"publisher","award":["PRIN 2017 project contract no. 2017ZBBYNC_002, Grant of Excellence Departments MI-UR-ARTICOLO 1, COMMI 314-337 LEGGE 232\/2016; Italy"],"award-info":[{"award-number":["PRIN 2017 project contract no. 2017ZBBYNC_002, Grant of Excellence Departments MI-UR-ARTICOLO 1, COMMI 314-337 LEGGE 232\/2016; Italy"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008991","name":"Universit\u00e1 degli Studi Roma Tre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008991","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Polyamines (PAs) are ubiquitous low-molecular-weight aliphatic compounds present in all living organisms and essential for cell growth and differentiation. The developmentally regulated and stress-induced copper amine oxidases (CuAOs) oxidize PAs to aminoaldehydes producing hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) and ammonia. The\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    CuAO\u03b2 (AtCuAO\u03b2) was previously reported to be involved in stomatal closure and early root protoxylem differentiation induced by the wound-signal MeJA\n                    <jats:italic>via<\/jats:italic>\n                    apoplastic H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    production, suggesting a role of this enzyme in water balance, by modulating xylem-dependent water supply and stomata-dependent water loss under stress conditions. Furthermore, AtCuAO\u03b2 has been shown to mediate early differentiation of root protoxylem induced by leaf wounding, which suggests a whole-plant systemic coordination of water supply and loss through stress-induced stomatal responses and root protoxylem phenotypic plasticity. Among apoplastic ROS generators, the D isoform of the respiratory burst oxidase homolog (RBOH) has been shown to be involved in stress-mediated modulation of stomatal closure as well. In the present study, the specific role of AtCuAO\u03b2 and RBOHD in local and systemic perception of leaf and root wounding that triggers stomatal closure was investigated at both injury and distal sites exploiting\n                    <jats:italic>Atcuao\u03b2<\/jats:italic>\n                    and\n                    <jats:italic>rbohd<\/jats:italic>\n                    insertional mutants. Data evidenced that AtCuAO\u03b2-driven H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    production mediates both local and systemic leaf-to-leaf and root-to-leaf responses in relation to stomatal movement,\n                    <jats:italic>Atcuao\u03b2<\/jats:italic>\n                    mutants being completely unresponsive to leaf or root wounding. Instead, RBOHD-driven ROS production contributes only to systemic leaf-to-leaf and root-to-leaf stomatal closure, with\n                    <jats:italic>rbohd<\/jats:italic>\n                    mutants showing partial unresponsiveness in distal, but not local, responses. Overall, data herein reported allow us to hypothesize that RBOHD may act downstream of and cooperate with AtCuAO\u03b2 in inducing the oxidative burst that leads to systemic wound-triggered stomatal closure.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2023.1154431","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:28:07Z","timestamp":1682036887000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Distinct role of AtCuAO\u03b2- and RBOHD-driven H2O2 production in wound-induced local and systemic leaf-to-leaf and root-to-leaf stomatal closure"],"prefix":"10.3389","volume":"14","author":[{"given":"Ilaria","family":"Fraudentali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Pedalino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"D\u2019Inc\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paraskevi","family":"Tavladoraki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Angelini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Cona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11122488","article-title":"Polyamine oxidase-generated reactive oxygen species in plant development and adaptation: the polyamine oxidase-NADPH oxidase nexus","volume":"11","author":"Benk\u0151","year":"2022","journal-title":"Antioxidants"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.615114","article-title":"Abscisic acid-induced stomatal closure: an important component of plant defense against abiotic and biotic stress","volume":"12","author":"Bharath","year":"2021","journal-title":"Front. 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