{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T09:09:29Z","timestamp":1793351369506,"version":"4.5.0"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Numerous studies have revealed the gasotransmitter functions of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) in various biological processes. However, the involvement of H<jats:sub>2<\/jats:sub>S in sulfur metabolism and\/or Cys synthesis makes its role as a signaling molecule ambiguous. The generation of endogenous H<jats:sub>2<\/jats:sub>S in plants is closely related to the metabolism of Cys, which play roles in a variety of signaling pathway occurring in various cellular processes. Here, we found that exogenous H<jats:sub>2<\/jats:sub>S fumigation and Cys treatment modulated the production rate and content of endogenous H<jats:sub>2<\/jats:sub>S and Cys to various degrees. Furthermore, we provided comprehensive transcriptomic analysis to support the gasotransmitter role of H<jats:sub>2<\/jats:sub>S besides as a substrate for Cys synthesis. Comparison of the differentially expressed genes (DEGs) between H<jats:sub>2<\/jats:sub>S and Cys treated seedlings indicated that H<jats:sub>2<\/jats:sub>S fumigation and Cys treatment caused different influences on gene profiles during seedlings development. A total of 261 genes were identified to respond to H<jats:sub>2<\/jats:sub>S fumigation, among which 72 genes were co-regulated by Cys treatment. GO and KEGG enrichment analysis of the 189 genes, H<jats:sub>2<\/jats:sub>S but not Cys regulated DEGs, indicated that these genes mainly involved in plant hormone signal transduction, plant-pathogen interaction, phenylpropanoid biosynthesis, and MAPK signaling pathway. Most of these genes encoded proteins having DNA binding and transcription factor activities that play roles in a variety of plant developmental and environmental responses. Many stress-responsive genes and some Ca<jats:sup>2+<\/jats:sup> signal associated genes were also included. Consequently, H<jats:sub>2<\/jats:sub>S regulated gene expression through its role as a gasotransmitter, rather than just as a substrate for Cys biogenesis, and these 189 genes were far more likely to function in H<jats:sub>2<\/jats:sub>S signal transduction independently of Cys. Our data will provide insights for revealing and enriching H<jats:sub>2<\/jats:sub>S signaling networks.<\/jats:p>","DOI":"10.3389\/fpls.2023.1184991","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T14:20:24Z","timestamp":1685715624000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Transcriptomic analysis reveals the functions of H2S as a gasotransmitter independently of Cys in Arabidopsis"],"prefix":"10.3389","volume":"14","author":[{"given":"Huihui","family":"Fang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kehong","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuke","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxi","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1104\/pp.109.147975","article-title":"An O-acetylserine(thiol)lyase homolog with l-cysteine desulfhydrase activity regulates cysteine homeostasis in arabidopsis","volume":"152","author":"Alvarez","year":"2010","journal-title":"Plant Physiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1093\/mp\/sss043","article-title":"Mitochondrial sulfide detoxification requires a functional isoform O-acetylserine(thiol)lyase c in arabidopsis thaliana","volume":"5","author":"Alvarez","year":"2012","journal-title":"Mol. 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