{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:52:35Z","timestamp":1793213555266,"version":"4.5.0"},"reference-count":82,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:00:00Z","timestamp":1720742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Naturally synthesized secondary metabolites in plants are considered an important source of drugs, food additives, etc. Among them, research on natural plant medicinal components and their synthesis mechanisms has always been of high concern. We identified a novel medicinal floral crop, <jats:italic>Plumbago auriculata<\/jats:italic> L., that can be treated with methyl jasmonate (MeJA) for the rapid or sustainable production of natural bioactives from hairy roots. In the study, we globally analyzed the changes in the accumulation of plumbagin and others in the hairy roots of <jats:italic>Plumbago auriculata<\/jats:italic> L. hairy roots (PAHR) 15834 in <jats:italic>P. auriculata<\/jats:italic> L. based on 100 \u03bcmol\/L of MeJA treatment by RNA-seq profiling, and we found that there was a significant increase in the accumulation of plumbagin and saponin before 24\u00a0h. To explain the principle of co-accumulation, it showed that MeJA induced JA signaling and the shikimic acid pathway, and the methylvaleric acid (MVA) pathway was activated downstream subsequently by the Mfuzz and weighted gene co-expression analysis. Under the shared metabolic pathway, the high expression of <jats:italic>PAL3<\/jats:italic> and <jats:italic>HMGR<\/jats:italic> promoted the activity of the \u201cgateway enzymes\u201d phenylalanine ammonia lyase (PAL) and 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), which respectively induced the high expression of key reaction enzyme genes, including chalcone synthase (CHS), isopentenyl diphosphate (IPP), and farnesyl pyrophosphate synthase (FPS), that led to the synthesis of plumbagin and saponin. We speculated that large amounts of ketones and\/or aldehydes were formed under the action of these characteristic enzymes, ultimately achieving their co-accumulation through polyketone and high-level sugar and amino acid metabolism. The study results provided a theoretical basis for carrying out the factory refinement and biosynthesis of plumbagin and saponins and also provided new ideas for fully exploiting multifunctional agricultural crops and plants and developing new agricultural by-products.<\/jats:p>","DOI":"10.3389\/fpls.2024.1411963","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T04:57:10Z","timestamp":1720760230000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["MeJA-induced hairy roots in Plumbago auriculata L. by RNA-seq profiling and key synthase provided new insights into the sustainable production of plumbagin and saponins"],"prefix":"10.3389","volume":"15","author":[{"given":"Yirui","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi-an","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiani","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suping","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,7,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1007\/s11240\u2013014-0666\u20132","article-title":"Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L","volume":"120","author":"Ali","year":"2014","journal-title":"Plant Cell Tissue Organ Culture (PCTOC)"},{"key":"B2","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/nature13084","article-title":"Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant","volume":"509","author":"Bonawitz","year":"2014","journal-title":"Nature"},{"key":"B3","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1016\/j.freeradbiomed.2019.09.001","article-title":"Modulation of Caspase-3 activity using a redox active vitamin K3 analogue, plumbagin, as a novel strategy for radioprotection","volume":"143","author":"Checker","year":"2019","journal-title":"Free Radical Biol. Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.plantsci.2007.03.013","article-title":"MeJA-induced transcriptional changes in adventitious roots of Bupleurum kaoi","volume":"173","author":"Chen","year":"2007","journal-title":"Plant Sci."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22010074","article-title":"Molecular cloning, characterization, and functional analysis of acetyl-coA C-acetyltransferase and mevalonate kinase genes involved in terpene trilactone biosynthesis from ginkgo biloba","volume":"22","author":"Chen","year":"2017","journal-title":"Molecules"},{"key":"B6","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.jare.2022.03.014","article-title":"Identification of oxidosqualene cyclases associated with saponin biosynthesis from Astragalus membranaceusreveals a conserved motif important for catalytic function","volume":"43","author":"Chen","year":"2023","journal-title":"J. Advanced Res."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2024.148163","article-title":"Integrated metabolome and transcriptome analysis reveals the regulatory mechanism of low nitrogen-driven biosynthesis of saponins and flavonoids in Panax notoginseng","volume":"901","author":"Cun","year":"2024","journal-title":"Gene."},{"key":"B8","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1111\/jipb.13054","article-title":"Contribution of phenylpropanoid metabolism to\u00a0plant development and plant\u2013environment interactions","volume":"63","author":"Dong","year":"2021","journal-title":"J. Integr. Plant Biol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"114798","DOI":"10.1016\/j.indcrop.2022.114798","article-title":"Transcriptome analysis reveals the effects of red and blue light on the physiological and medicinal components of Dendrobium denneanum","volume":"180","author":"Fan","year":"2022","journal-title":"Ind. Crops Products"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1082554","article-title":"Exogenous application of stress-related signaling molecules affect growth and cannabinoid accumulation in medical cannabis (Cannabis sativa L.)","volume":"13","author":"Garrido","year":"2022","journal-title":"Front. Plant Sci."},{"key":"B11","doi-asserted-by":"publisher","first-page":"2303","DOI":"10.1080\/10408398.2019.1634517","article-title":"Hypoglycemic effects of bioactive ingredients from medicine food homology and medicinal health food species used in China","volume":"60","author":"Gong","year":"2020","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2019.1642038","article-title":"Molecular mechanism of plant stress hormone methyl jasmonate for its anti-inflammatory activity","volume":"14","author":"Gunjegaonkar","year":"2019","journal-title":"Plant Signaling Behav."},{"key":"B13","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1039\/D0FO01896A","article-title":"Recent progress in polysaccharides from Panax ginseng C. A. Meyer","volume":"12","author":"Guo","year":"2021","journal-title":"Food Funct."},{"key":"B14","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/S1369-5266(00)00114-X","article-title":"Sorting of soluble proteins in the secretory pathway of plants","volume":"3","author":"Hadlington","year":"2000","journal-title":"Curr. Opin. Plant Biol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/S0031-9422(01)00296-5","article-title":"Biosynthesis of anthraquinones in cell cultures of Cinchona \u2018Robusta\u2019 proceeds via the methylerythritol 4-phosphate pathway","volume":"59","author":"Han","year":"2002","journal-title":"Phytochemistry"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1111\/nph.18306","article-title":"Comparison of buckwheat genomes reveals the genetic basis of metabolomic divergence and ecotype differentiation","volume":"235","author":"He","year":"2022","journal-title":"New Phytol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"438","DOI":"10.3724\/sp.j.1145.2014.04028","article-title":"Contents of polyphenols and flavonoids in Dendrobium candidum and their correlation with antioxidant activity","volume":"20","author":"Huang","year":"2014","journal-title":"Chin. J. Appl. Environ. Biol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.phytochem.2013.11.017","article-title":"Polyketide synthesis in tobacco plants transformed with a Plumbago zeylanica type III hexaketide synthase","volume":"98","author":"Jadhav","year":"2014","journal-title":"Phytochemistry"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3390\/foods9020142","article-title":"The pharmacological effects and health benefits of platycodon grandiflorus\u2014A medicine food homology species","volume":"9","author":"Ji","year":"2020","journal-title":"Foods"},{"key":"B20","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.bej.2015.10.010","article-title":"Enhanced astragaloside production and transcriptional responses of biosynthetic genes in Astragalus membranaceus hairy root cultures by elicitation with methyl jasmonate","volume":"105","author":"Jiao","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"B21","doi-asserted-by":"publisher","first-page":"3043","DOI":"10.1016\/j.phytochem.2008.03.013","article-title":"Pyrone polyketides synthesized by a type III polyketide synthase from Drosophyllum lusitanicum","volume":"69","author":"Jindaprasert","year":"2008","journal-title":"Phytochemistry"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0300895","article-title":"HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd","volume":"19","author":"Joga","year":"2024","journal-title":"PloS One"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061310","article-title":"Abscisic acid regulates the 3-hydroxy-3-methylglutaryl coA reductase gene promoter and ginsenoside production in panax quinquefolium hairy root cultures","volume":"20","author":"Kochan","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"117140","DOI":"10.1016\/j.indcrop.2023.117140","article-title":"Enhanced production of bioactive plumbagin in hairy root cultures and adventitious root cultures of Plumbago zeylanica L. by a novel apocarotenoid elicitor, \u03b1-ionone","volume":"203","author":"Kumar","year":"2023","journal-title":"Ind. Crops Products"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00735","article-title":"Glycosylation is a major regulator of phenylpropanoid availability and biological activity in plants","volume":"7","author":"Le Roy","year":"2016","journal-title":"Front. Plant Sci."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0236565","article-title":"Two responses to MeJA induction of R2R3-MYB transcription factors regulate flavonoid accumulation in Glycyrrhiza uralensis Fisch","volume":"15","author":"Li","year":"2020","journal-title":"PloS One"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1149669","article-title":"ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L","volume":"14","author":"Li","year":"2023","journal-title":"Front. Plant Sci."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.760460","article-title":"Transcriptome Analysis Revealed a Cold Stress-Responsive Transcription Factor, PaDREB1A, in Plumbago auriculata That Can Confer Cold Tolerance in Transgenic Arabidopsis thaliana","volume":"13","author":"Li","year":"2022","journal-title":"Front. Plant Sci."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-020-03082-4","article-title":"Transcriptome profiling of Plumbago auriculata Lam. in response to cold stress","volume":"42","author":"Li","year":"2020","journal-title":"Acta Physiologiae Plantarum"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.713795","article-title":"Transcriptomic and metabolomic analyses reveals that exogenous methyl jasmonate regulates galanthamine biosynthesis in lycoris longituba seedlings","volume":"12","author":"Li","year":"2021","journal-title":"Front. Plant Sci."},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032620","article-title":"Advances in the biosynthesis and molecular evolution of steroidal saponins in plants","volume":"24","author":"Li","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-024-04861-8","article-title":"Integrated metabolome and transcriptome analyses reveal the molecular mechanism underlying dynamic metabolic processes during taproot development of Panax notoginseng","volume":"24","author":"Li","year":"2024","journal-title":"BMC Plant Biol."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-023-02393-w","article-title":"Anti-ovarian cancer actions and pharmacological targets of plumbagin","volume":"396","author":"Liang","year":"2023","journal-title":"Naunyn-Schmiedebergs Arch. Pharmacol"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhab025","article-title":"Photoprotection contributes to freezing tolerance as revealed by RNA-seq profiling of Rhododendron leaves during cold acclimation and deacclimation over time","volume":"9","author":"Liu","year":"2022","journal-title":"Horticulture Res."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1114345","article-title":"Molecular identification of phenylalanine ammonia lyase-encoding genes EfPALs and EfPAL2-interacting transcription factors in Euryale ferox","volume":"14","author":"Liu","year":"2023","journal-title":"Front. Plant Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"2292","DOI":"10.1111\/1751-7915.14072","article-title":"Enhancing fluxes through the mevalonate pathway in Saccharomyces cerevisiae by engineering the HMGR and \u03b2-alanine metabolism","volume":"15","author":"Lu","year":"2022","journal-title":"Microbial Biotechnol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1111\/nph.14638","article-title":"Regulation of growth\u2013defense balance by the JASMONATE ZIM-DOMAIN (JAZ)-MYC transcriptional module","volume":"215","author":"Major","year":"2017","journal-title":"New Phytol."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3390\/life13020563","article-title":"Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in Bupleurum chinense DC","volume":"13","author":"Mao","year":"2023","journal-title":"Life"},{"key":"B39","doi-asserted-by":"publisher","first-page":"103457","DOI":"10.1016\/j.fbio.2023.103457","article-title":"Exploring novel insights: Methyl jasmonate treatment reveals novel lncRNA-mediated regulation of secondary metabolite biosynthesis pathways in EChinacea purpurea","volume":"57","author":"Moghadam","year":"2024","journal-title":"Food Bioscience"},{"key":"B40","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.phytochem.2016.12.007","article-title":"ABA and GA3 regulate the synthesis of primary and secondary metabolites related to alleviation from biotic and abiotic stresses in grapevine","volume":"135","author":"Murcia","year":"2017","journal-title":"Phytochemistry"},{"key":"B41","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.phytochem.2008.10.010","article-title":"Arabidopsis 3-hydroxy-3-methylglutaryl-CoA reductase is regulated at the post-translational level in response to alterations of the sphingolipid and the sterol biosynthetic pathways","volume":"70","author":"Nieto","year":"2009","journal-title":"Phytochemistry"},{"key":"B42","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.plaphy.2013.10.035","article-title":"Differential induction of antioxidant stilbenoids in hairy roots of Vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide","volume":"74","author":"Nopo-Olazabal","year":"2014","journal-title":"Plant Physiol. Biochem."},{"key":"B43","doi-asserted-by":"publisher","first-page":"307","DOI":"10.2147\/JIR","article-title":"Medicinal plants with anti-inflammatory activities from selected countries and regions of Africa","author":"Oguntibeju","year":"2018","journal-title":"J. Inflammation Res."},{"key":"B44","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1038\/nature12685","article-title":"The protein quality control system manages plant defence compound synthesis","volume":"504","author":"Pollier","year":"2013","journal-title":"Nature"},{"key":"B45","doi-asserted-by":"publisher","first-page":"12173","DOI":"10.3390\/ijms232012173","article-title":"The influence of exogenous jasmonic acid on the biosynthesis of steroids and triterpenoids in calendula officinalis plants and hairy root culture","volume":"23","author":"Rogowska","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"B46","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1104\/pp.113.1.175","article-title":"Maize phenylalanine ammonia-lyase has tyrosine ammonia-lyase activity","volume":"113","author":"Rosler","year":"1997","journal-title":"Plant Physiol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1093\/pcp\/pcv062","article-title":"P450s and UGTs: key players in the structural diversity of triterpenoid saponins","volume":"56","author":"Seki","year":"2015","journal-title":"Plant Cell Physiol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"2738","DOI":"10.3390\/cimb45040179","article-title":"Transcriptomics reveals the molecular basis for methyl jasmonate to promote the synthesis of monoterpenoids in schizonepeta tenuifolia briq","volume":"45","author":"Shi","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1111\/cbdd.14305","article-title":"Plumbagin induces apoptosis, cell cycle arrest, and inhibits protein synthesis in LoVo colon cancer cells: A proteomic analysis","volume":"102","author":"Shu","year":"2023","journal-title":"Chem. Biol. Drug Design"},{"key":"B50","doi-asserted-by":"publisher","first-page":"839","DOI":"10.15835\/nbha48211891","article-title":"Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors","volume":"48","author":"Shuang","year":"2020","journal-title":"Notulae Botanicae Horti Agrobotanici Cluj-Napoca"},{"key":"B51","doi-asserted-by":"publisher","first-page":"199","DOI":"10.21010\/ajtcam.v15i1.21","article-title":"A comprehensive review on the genus plumbago with focus on plumbago auriculata (Plumbaginaceae)","volume":"15","author":"Singh","year":"2017","journal-title":"Afr. J. Traditional Complementary Altern. Medicines"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.942789","article-title":"Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview","volume":"13","author":"Sohn","year":"2022","journal-title":"Front. Plant Sci."},{"key":"B53","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1111\/j.1742-4658.2006.05588.x","article-title":"A polyketide synthase of Plumbago indica that catalyzes the formation of hexaketide pyrones","volume":"274","author":"Springob","year":"2006","journal-title":"FEBS J."},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-262","article-title":"De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis","volume":"11","author":"Sun","year":"2010","journal-title":"BMC Genomics"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-018-2611-1","article-title":"Methyl jasmonate effects\u00a0on volatile oil compounds and antioxidant activity of leaf extract of two basil cultivars under salinity stress","volume":"40","author":"Talebi","year":"2018","journal-title":"Acta Physiologiae Plantarum"},{"key":"B56","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/10_2014_295","article-title":"Biosynthesis and Biological Functions of Terpenoids in Plants","volume-title":"Biotechnology of Isoprenoids","author":"Tholl","year":"2015"},{"key":"B57","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1093\/jaoac\/83.6.1451","article-title":"Spectrophotometric determination of saponin in yucca extract used as food additive","volume":"83","author":"Uematsu","year":"2019","journal-title":"J. AOAC Int."},{"key":"B58","doi-asserted-by":"publisher","first-page":"104761","DOI":"10.1016\/j.fitote.2020.104761","article-title":"Differential transcriptome and\u00a0metabolome analysis of Plumbago zeylanica L. reveal putative genes involved in plumbagin biosynthesis","volume":"147","author":"Vasav","year":"2020","journal-title":"Fitoterapia"},{"key":"B59","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1007\/s11105-012-0523-6","article-title":"Molecular cloning, biochemical characterization, and differential expression of an acetyl-coA C-acetyltransferase gene (AACT) of brahmi (Bacopa monniera)","volume":"31","author":"Vishwakarma","year":"2013","journal-title":"Plant Mol. Biol. Rep."},{"key":"B60","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1093\/mp\/ssp106","article-title":"Phenylpropanoid biosynthesis","volume":"3","author":"Vogt","year":"2010","journal-title":"Mol. Plant"},{"key":"B61","doi-asserted-by":"publisher","first-page":"111762","DOI":"10.1016\/j.plantsci.2023.111762","article-title":"Combined transcriptomic and metabolomic analyses identifies CsERF003, a citrus ERF transcription factor, as flavonoid activator","volume":"334","author":"Wan","year":"2023","journal-title":"Plant Sci."},{"key":"B62","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1093\/pcp\/pcy211","article-title":"Plant primary metabolism regulated by nitrogen contributes to plant\u2013pathogen interactions","volume":"60","author":"Wang","year":"2019","journal-title":"Plant Cell Physiol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1111\/tpj.14364","article-title":"R2R3-MYB transcription factor MYB6 promotes anthocyanin and proanthocyanidin biosynthesis but inhibits secondary cell wall formation in Populus tomentosa","volume":"99","author":"Wang","year":"2019","journal-title":"Plant J."},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22168568","article-title":"Function and mechanism of jasmonic acid in plant responses to abiotic and biotic stresses","volume":"22","author":"Wang","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03102","article-title":"Plumbagin ameliorates collagen-induced arthritis by regulating treg\/th17 cell imbalances and suppressing osteoclastogenesis","volume":"9","author":"Wang","year":"2019","journal-title":"Front. Immunol."},{"key":"B66","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.nbt.2017.09.007","article-title":"Jasmonates are signals in the biosynthesis of secondary metabolites \u2014 Pathways, transcription factors and applied aspects \u2014 A brief review","volume":"48","author":"Wasternack","year":"2019","journal-title":"New Biotechnol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"16046","DOI":"10.1038\/hortres.2016.46","article-title":"Biosynthesis and molecular actions of specialized 1,4-naphthoquinone natural products produced by horticultural plants","volume":"3","author":"Widhalm","year":"2016","journal-title":"Horticulture Res."},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14008","article-title":"GhWRKY41 forms a positive feedback regulation loop and increases cotton defence response against Verticillium dahliae by regulating phenylpropanoid metabolism","volume":"21","author":"Xiao","year":"2023","journal-title":"Plant Biotechnol. J"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18010096","article-title":"Natural antioxidants in foods and medicinal plants: extraction, assessment and resources","volume":"18","author":"Xu","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"B70","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.indcrop.2018.04.010","article-title":"Longitudinal expression patterns of HMGR, FPS, SS, SE and DS and their correlations with saponin contents in green-purple transitional aerial stems of Panax notoginseng","volume":"119","author":"Xu","year":"2018","journal-title":"Ind. Crops Products"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.3390\/genes13010067","article-title":"Transcriptome analysis reveals differentially expressed genes that regulate biosynthesis of the active compounds with methyl jasmonate in rosemary suspension cells","volume":"13","author":"Yao","year":"2021","journal-title":"Genes"},{"key":"B72","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1093\/oxfordjournals.pcp.a029235","article-title":"Effects of methyl jasmonate on shikonin and dihydroechinofuran production in Lithospermum cell cultures","volume":"38","author":"Yazaki","year":"1997","journal-title":"Plant Cell Physiol."},{"key":"B73","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.ymben.2023.02.009","article-title":"Deciphering the network of cholesterol biosynthesis in Paris polyphylla laid a base for efficient diosgenin production in plant chassis","volume":"76","author":"Yin","year":"2023","journal-title":"Metab. Eng."},{"key":"B74","doi-asserted-by":"publisher","first-page":"(1)","DOI":"10.1186\/s12870-024-05090-9","article-title":"Integrated transcriptome and metabolome analysis reveals the regulation of phlorizin synthesis in Lithocarpus polystachyus under nitrogen fertilization","volume":"24","author":"Zeng","year":"2024","journal-title":"BMC Plant Biol."},{"key":"B75","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.molp.2014.11.001","article-title":"Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids","volume":"8","author":"Zhang","year":"2015","journal-title":"Mol. Plant"},{"key":"B76","doi-asserted-by":"publisher","first-page":"102","DOI":"10.15586\/qas.v13i3.960","article-title":"Plumbagin attenuates high glucose-induced trophoblast cell apoptosis and insulin resistance via activating AKT\/mTOR pathway","volume":"13","author":"Zhang","year":"2021","journal-title":"Qual. Assur. Saf. Crops Foods"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1186\/s40643-023-00656-1","article-title":"Hydroxylases involved in terpenoid biosynthesis: a review","volume":"10","author":"Zhang","year":"2023","journal-title":"Bioresources Bioprocessing"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhae058","article-title":"RNA sequencing analysis reveals PgbHLH28 as the key regulator in response to methyl jasmonate-induced saponin accumulation in Platycodon grandiflorus","volume":"11","author":"Zhang","year":"2024","journal-title":"Horticulture Res."},{"key":"B79","doi-asserted-by":"publisher","first-page":"137958","DOI":"10.1016\/j.foodchem.2023.137958","article-title":"Preharvest application of MeJA enhancing the quality of postharvest grape berries via regulating terpenes biosynthesis and phenylpropanoid metabolisms","volume":"438","author":"Zhang","year":"2024","journal-title":"Food Chem."},{"key":"B80","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/s11240-023-02553-z","article-title":"The hairy root induction efficiency and that plumbagin of Plumbago auriculata L","volume":"155","author":"Zhao","year":"2023","journal-title":"Plant Cell Tissue Organ Culture (PCTOC)"},{"key":"B81","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1007\/s11104-021-05110-9","article-title":"Fe induces a dynamic and biased allocation of material flux within terpenoid metabolism controlled by CbNudix in Conyza blinii","volume":"467","author":"Zheng","year":"2021","journal-title":"Plant Soil"},{"key":"B82","doi-asserted-by":"publisher","first-page":"154218","DOI":"10.1016\/j.jplph.2024.154218","article-title":"Identification of transcription factor genes responsive to MeJA and characterization of a LaMYC2 transcription factor positively regulates lycorine biosynthesis in Lycoris aurea","volume":"296","author":"Zhou","year":"2024","journal-title":"J. Plant Physiol."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2024.1411963\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T04:57:14Z","timestamp":1720760234000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2024.1411963\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,12]]},"references-count":82,"alternative-id":["10.3389\/fpls.2024.1411963"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2024.1411963","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,12]]},"article-number":"1411963"}}