{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:33:57Z","timestamp":1784309637234,"version":"3.55.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Magnesium (Mg) is a crucial macronutrient for plants. Understanding the molecular responses of plants to different levels of Mg supply is important for improving cultivation practices and breeding new varieties with efficient Mg utilization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we conducted a comprehensive transcriptome analysis on tobacco (<jats:italic>Nicotiana tabacum<\/jats:italic> L.) seedling leaves to investigate changes in gene expression in response to different levels of Mg supply, including Mg-deficient, 1\/4-normal Mg, normal Mg, and 4\u00d7-normal Mg, with a particular focus on Mg deficiency at 5, 15 and 25 days after treatment (DAT), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 11,267 differentially expressed genes (DEGs) were identified in the Mg-deficient, 1\/4-normal Mg, and\/or 4\u00d7-normal Mg seedlings compared to the normal Mg seedlings. The global gene expression profiles revealed potential mechanisms involved in the response to Mg deficiency in tobacco leaves, including down-regulation of genes\u2013two DEGs encoding mitochondria-localized NtMGT7 and NtMGT9 homologs, and one DEG encoding a tonoplast-localized NtMHX1 homolog\u2013associated with Mg trafficking from the cytosol to mitochondria and vacuoles, decreased expression of genes linked to photosynthesis and carbon fixation at later stages, and up-regulation of genes related to antioxidant defenses, such as <jats:italic>NtPODs<\/jats:italic>, <jats:italic>NtPrxs<\/jats:italic>, and <jats:italic>NtGSTs<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Our findings provide new insights into the molecular mechanisms underlying how tobacco responds to Mg deficiency.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fpls.2024.1483217","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T04:23:52Z","timestamp":1732508632000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Integrative transcriptomic analysis reveals the molecular responses of tobacco to magnesium 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