{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T17:07:12Z","timestamp":1782580032870,"version":"3.54.5"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Taste is one of the vital indicators for the quality evaluation of <jats:italic>Coptis<\/jats:italic> rhizome (CR), but the traditional taste evaluation lacks objectivity. By establishing the correlation between CR\u2019s tastes and alkaloids, an objective basis for the taste evaluation was established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-QQQ-MS\/MS) and electronic tongue technique were performed to determine ten alkaloid contents and eight tastes from <jats:italic>Coptis chinensis<\/jats:italic> rhizome, <jats:italic>Coptis deltoidea<\/jats:italic> rhizome, <jats:italic>Coptis teeta<\/jats:italic> rhizome, and <jats:italic>Coptis japonica<\/jats:italic> rhizome, respectively. Combined with multivariate statistical analysis, we established models to discriminate the alkaloid contents and tastes of CR, screened the differential alkaloids and tastes, and performed Pearson\u2019s correlation analysis on the results of alkaloids and tastes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>1) According to the previous UHPLC-QQQ-MS\/MS method established by our research group, the contents of ten alkaloids of the four species of CR were quantified, of which jatrorrhizine, columbamine, and magnoflorine were the differential alkaloids of the four species. 2) The electronic tongue technique realized the objectification of CR\u2019s tastes and distinguished the species of CR based on the tastes of aftertaste-A, sourness, bitterness, and richness. 3) Pearson\u2019s correlation analysis shows the bitterness of CR was mainly manifested as aftertaste-B, indicating the higher the aftertaste-B value, the higher the berberrubine content. Astringency and aftertaste-A could be suggested as quality evaluation indexes of CR, due to the positively correlated or significantly positively correlated with coptisine, epiberberine, berberine, and palmatine, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Electronic tongue technique has successfully achieved the objectification of the tastes of CR, and combined with UHPLC-QQQ-MS\/MS technique for alkaloid quantification and correlation research, it provides a new idea for the quality evaluation of CR.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fpls.2024.1496789","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T04:32:03Z","timestamp":1730953923000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Study on the correlation between alkaloids and tastes of Coptis Rhizome from four species based on UHPLC-QQQ-MS\/MS combined with electronic tongue technique"],"prefix":"10.3389","volume":"15","author":[{"given":"Yufeng","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhan","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"B1","volume-title":"Pharmacopoeia of the People\u2019s Republic of China, (2020, Part 1)","year":"2020"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1092643","article-title":"The localization of the alkaloids in Coptis chinensis rhizome by time-of-flight secondary ion mass spectrometry","volume":"13","author":"He","year":"2022","journal-title":"Front. 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