{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:12:26Z","timestamp":1793146346020,"version":"4.5.0"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The <jats:italic>Cannabis Sativa<\/jats:italic> L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The extraction parameters for CBD from industrial hemp were optimized using the Box-Behnken design and response surface methodology (RSM). The purification process involved characterizing the penetration and desorption profiles of CBD on HPD-100 resin. The <jats:italic>in vitro<\/jats:italic> antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC) against <jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic>. Antioxidant properties were evaluated using DPPH and ABTS assays, as well as an iron-reducing ability test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After optimization, the extraction rate of CBD reached 0.26 \u00b1 0.02%. The use of HP-100 resin in the purification process resulted in a significant enrichment of CBD content, which was 4.2 times higher than that of the crude extract, with a recovery rate of 83.13%. The MIC against <jats:italic>S. aureus<\/jats:italic> was found to be 5 mg\/mL, while no inhibitory effect was observed against <jats:italic>E. coli<\/jats:italic>. The IC50 values for the DPPH and ABTS assays were 0.1875 mg\/mL and 2.988 mg\/mL, respectively, indicating the potent antioxidant capacity of CBD. Additionally, CBD demonstrated a strong iron-reducing ability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings contribute to the development of CBD for broader applications in various industries, highlighting its potential as a valuable compound in health and wellness sectors.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fpls.2025.1495779","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T06:51:39Z","timestamp":1738911099000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity"],"prefix":"10.3389","volume":"16","author":[{"given":"Junkai","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunlai","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1016\/j.sjbs.2018.07.013","article-title":"Ultrasound-assisted extraction of some branded tea: Optimization based on polyphenol content, antioxidant potential and thermodynamic study","volume":"26","author":"Afroz Bakht","year":"2019","journal-title":"Saudi J. 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