{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:19:42Z","timestamp":1753892382908,"version":"3.41.2"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of glycerophosphodiesters into sn-glycerol-3-phosphate (G-3-P) and corresponding alcohols, which is integral to various physiological processes in plants. However, our comprehension of the <jats:italic>GDPD<\/jats:italic> gene family in foxtail millet (<jats:italic>Setaria italica<\/jats:italic> L.) remains limited and unclear. This study aimed to identify and predict the function of <jats:italic>GDPD<\/jats:italic> gene family members in foxtail millet through a comprehensive genome-wide analysis.14 <jats:italic>SiGDPD<\/jats:italic> genes were identified in the foxtail millet genome. Phylogenetic analysis categorized <jats:italic>SiGDPD<\/jats:italic> proteins into five groups. Promoter regions of <jats:italic>SiGDPD<\/jats:italic> genes contained multiple <jats:italic>cis<\/jats:italic>-acting elements related to light response, hormone regulation, and stress response. Phylogenetic and collinearity analyses demonstrated conservation of GDPD proteins among foxtail millet, sorghum, rice, and maize, with the <jats:italic>SiGDPD<\/jats:italic> gene family undergoing purifying selection during evolution.Tissue differential expression analysis revealed distinct expression patterns of <jats:italic>SiGDPD<\/jats:italic> genes across various tissues, showing spatiotemporal expression characteristics. Under low phosphorus stress, the expression levels of <jats:italic>SiGDPD3<\/jats:italic> and <jats:italic>SiGDPD14<\/jats:italic> significantly increased, while <jats:italic>SiGDPD1<\/jats:italic>, <jats:italic>SiGDPD5<\/jats:italic>, <jats:italic>SiGDPD6<\/jats:italic>, and <jats:italic>SiGDPD11<\/jats:italic> showed significant decreases.To identify the function of <jats:italic>SiGDPD14<\/jats:italic>, an over-expressed transgenic <jats:italic>Arabidopsis<\/jats:italic> was generated. The results showed that transgenic <jats:italic>Arabidopsis thaliana<\/jats:italic> plants over-expressing <jats:italic>SiGDPD14<\/jats:italic> exhibited enhanced tolerance to low phosphorus stress.Taken together, the results of this study provide valuable information for further studies on candidate <jats:italic>SiGDPD<\/jats:italic> genes involved in the phosphate deficiency response in foxtail millet.<\/jats:p>","DOI":"10.3389\/fpls.2025.1586547","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T05:48:00Z","timestamp":1750225680000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification of GDPD gene family in foxtail millet (Setaria italica L.) and functional characterization of SiGDPD14 under low phosphorus stress"],"prefix":"10.3389","volume":"16","author":[{"given":"Chaomin","family":"Meng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haojie","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Furong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"W202","DOI":"10.1093\/nar\/gkp335","article-title":"MEME SUITE: tools for motif discovery and searching","volume":"37","author":"Bailey","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: an integrative toolkit developed for interactive analyses of big biological data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol. 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