{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:15:06Z","timestamp":1791317706490,"version":"4.3.1"},"reference-count":114,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    <jats:italic>Bucephalandra<\/jats:italic>\n                    sp. \u2018Wavy Dark Green\u2019 is a commercially valuable ornamental aquatic plant with limited propagation potential due to low multiplication rates and acclimatization challenges. This study aimed to developed an integrated tissue culture protocol by optimizing disinfection procedures, basal media and plant growth regulator, temporary immersion system (TIS) conditions, light quality, and rooting and acclimatization methods. Surface sterilization using 0.1\u20130.2% HgCl\n                    <jats:sub>2<\/jats:sub>\n                    or a two-step 1.2% NaOCl protocol significantly improved explant survival and reduced contamination. MS medium supplemented with 5 mg\/L benzylaminopurine (BA) produced the highest shoot proliferation on solid culture media. In TIS, six immersions per day for 2\u20133 minutes yielded optimal shoot multiplication and biomass. Blue:red (70:30) and pure blue light enhanced chlorophyll content, photosynthetic efficiency (QY), stomatal opening, and upregulated key light-responsive genes (\n                    <jats:italic>psaA<\/jats:italic>\n                    ,\n                    <jats:italic>psbB<\/jats:italic>\n                    ,\n                    <jats:italic>petG<\/jats:italic>\n                    ,\n                    <jats:italic>pskB<\/jats:italic>\n                    ,\n                    <jats:italic>atpA<\/jats:italic>\n                    ,\n                    <jats:italic>petA<\/jats:italic>\n                    ). Rooting was most effective in \u00bc or full-strength MS medium with 0.3 mg\/L naphthaleneacetic acid (NAA). Acclimatized plantlets grown in sand exhibited the highest survival and growth. This integrated protocol supports efficient micropropagation and\n                    <jats:italic>ex vitro<\/jats:italic>\n                    establishment of\n                    <jats:italic>Bucephalandra<\/jats:italic>\n                    sp. \u2018Wavy Dark Green\u2019, facilitating its conservation, ornamental use, and sustainable export, in line with SDG 12 (Responsible Consumption and Production).\n                  <\/jats:p>","DOI":"10.3389\/fpls.2025.1660632","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:16:18Z","timestamp":1764688578000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Light spectrum effects on micropropagation and gene expression of Bucephalandra sp. in a temporary immersion system for sustainable production"],"prefix":"10.3389","volume":"16","author":[{"given":"Chanakan","family":"Laksana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onsulang","family":"Sophiphun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paitoon","family":"Kaewhom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sontichai","family":"Chanprame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","DOI":"10.1007\/1-4020-3200-5_33","article-title":"Use of the temporary immersion bioreactor system (RITA\u00ae) for production of commercial Eucalyptus clones in Mondi Forests (SA)","volume-title":"Liquid Culture Systems for","author":"Alister","year":"2005"},{"key":"B2","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/BF00040116","article-title":"Comparison of temporary immersion and permanent immersion systems for banana micropropagation","volume":"32","author":"Alvard","year":"1993","journal-title":"Plant Cell Tis Organ Cult."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/3815651","article-title":"Application of red and blue LED light on cultivation and postharvest of tomatoes (Solanum lycopersicum L.)","volume":"2024","author":"Arif","year":"2024","journal-title":"Scientifica (Cairo)"},{"key":"B4","first-page":"13","article-title":"Sterilization of Anubias nana and Rotala macrandra for in vitro culture","volume":"10","author":"Azmi","year":"2019","journal-title":"J. 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