{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:20:08Z","timestamp":1789676408175,"version":"4.0.1"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    The genus\n                    <jats:italic>Eryngium<\/jats:italic>\n                    , belonging to the Umbelliferae family, comprises flowering plants with various pharmacological activities, including anti-inflammatory and antidiabetic properties. However, many of these activities lack scientific evaluation. This study aimed to characterize the metabolites and evaluate the antihelmintic, antibacterial, and antibiofilm activities of a methanolic extract derived from the aerial parts of\n                    <jats:italic>Eryngium billardieri<\/jats:italic>\n                    . Metabolite characterization was conducted using LC-MS combined with a computer-assisted structure elucidation method. The extract was tested against six fungi, six Gram-positive bacteria, and nine Gram-negative bacteria, and a non-parasitic nematode (\n                    <jats:italic>Caenorhabditis elegans)<\/jats:italic>\n                    . A total of thirty-three compounds were identified, with the major constituents including isorhamnetin-3-O-glucoside, phytolaccagenin, terpinolene, 3,4-dimethoxybenzaldehyde, palmitic acid, isobornyl formate, isorhamnetin, and 1,4-dimethyl-7-(1-methylethenyl)-octahydroazulene. Across all tested concentrations, Gram-positive bacteria demonstrated greater sensitivity compared to Gram-negative bacteria, with\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and\n                    <jats:italic>Micrococcus luteus<\/jats:italic>\n                    showing the highest sensitivity (IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 57.47 \u00b5g\/mL and 105.8 \u00b5g\/mL, respectively). Among Gram-negative strains, only\n                    <jats:italic>Brevundimonas diminuta<\/jats:italic>\n                    exhibited sensitivity. In antifungal tests, six of seven yeast strains displayed sensitivity to the extract, with\n                    <jats:italic>Candida parapsilosis<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    being particularly susceptible (IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 11.29 \u00b5g\/mL and 63.29 \u00b5g\/mL, respectively). The antibiofilm analysis demonstrated inhibitory effects within 24 hours after biofilm formation, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 6.3 \u00b5g\/mL. Additionally, the antihelmintic assay revealed a mean inhibition rate of 97.7\u00a0\u00b1\u00a01.5 at 2.0 \u00b5g\/mL. The results demonstrate that the extract effectively inhibited the tested bacteria, particularly against yeast strains. While the extract showed promising activity against a model nematode, further research is imperative to validate its anthelmintic efficacy against parasitic nematodes.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2025.1667335","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T13:27:07Z","timestamp":1762435627000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of phytochemical profiling and biological activities of methanol extract from Eryngium billardieri: antimicrobial, antibiofilm, and anthelmintic properties"],"prefix":"10.3389","volume":"16","author":[{"given":"Mahdi","family":"Yaghoobi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Moridi Farimani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajmal","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojtaba","family":"Asadollahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzieh","family":"Omrani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Luyten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30081751","article-title":"The potential therapeutic role of beta\u2212caryophyllene as a chemosensitizer and an inhibitor of angiogenesis in cancer","volume":"30","author":"Ahmed","year":"2025","journal-title":"Molecules"},{"key":"B2","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-63862-1","volume-title":"Therapeutic use of medicinal plants and their extracts: volume 1","author":"Alamgir","year":"2017"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2022.139385","article-title":"Antibacterial and cytotoxic effects of silver nanoparticles fabricated by Eryngium billardieri Delar. extract","volume":"791","author":"Allafchian","year":"2022","journal-title":"Chem. 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