{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T04:27:01Z","timestamp":1786508821005,"version":"build-2736575974"},"reference-count":88,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The vascular cambium is a pivotal tissue that drives secondary growth in woody plants, directly determining wood structure and properties.\n                      <jats:italic>Schima superba<\/jats:italic>\n                      , a valuable timber species in China, holds considerable ecological and economic importance. However, the seasonal activity of its cambium remains poorly understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To elucidate the underlying molecular and metabolic regulatory mechanisms, we integrated untargeted metabolomics, lignin-targeted metabolomics, and transcriptomic analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Untargeted metabolomic profiling identified 2,178 differentially synthesised metabolites (DSMs), predominantly associated with amino acid metabolism, fatty acid metabolism, phenylpropanoid biosynthesis (PB), and plant hormone signal transduction (PHST). Lignin-targeted metabolomics detected 8 DSMs, most of which initially increased and subsequently declined, peaking in August, with the exception of sinapyl alcohol and L-phenylalanine. Transcriptomic analysis identified 19,609 differentially expressed genes (DEGs), primarily enriched in PB and PHST pathways. Key PB-related structural genes, including\n                      <jats:italic>SsPAL<\/jats:italic>\n                      ,\n                      <jats:italic>SsC4H<\/jats:italic>\n                      ,\n                      <jats:italic>Ss4CL<\/jats:italic>\n                      ,\n                      <jats:italic>SsCOMT<\/jats:italic>\n                      , and\n                      <jats:italic>SsCAD<\/jats:italic>\n                      , exhibited peak expression during the cambial active period, correlating with lignin intermediate accumulation and indicating tight coordination between transcriptional activity and metabolic flux during lignification. PHST-related genes involved in auxin (AUX), cytokinin (CTK), and gibberellin (GA) signalling, such as\n                      <jats:italic>SsARF<\/jats:italic>\n                      ,\n                      <jats:italic>SsSAUR<\/jats:italic>\n                      , and\n                      <jats:italic>SsARR-B<\/jats:italic>\n                      , displayed stage-specific expression patterns regulating cambial cell expansion and division. Weighted gene coexpression network analysis (WGCNA) further identified core transcription factors, including\n                      <jats:italic>SsMYB<\/jats:italic>\n                      ,\n                      <jats:italic>SsbHLH<\/jats:italic>\n                      ,\n                      <jats:italic>SsWOX<\/jats:italic>\n                      ,\n                      <jats:italic>SsGRAS<\/jats:italic>\n                      ,\n                      <jats:italic>SsSAUR<\/jats:italic>\n                      , and\n                      <jats:italic>SsNF-YB<\/jats:italic>\n                      , as regulators of seasonal cambial activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      These findings provide a comprehensive framework for understanding seasonal cambial activity in\n                      <jats:italic>S. superba<\/jats:italic>\n                      , and offer valuable insights into the regulatory mechanisms of vascular cambium activity in woody plants, thereby supporting targeted wood quality improvement and advancing our understanding of plant developmental biology.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2025.1727826","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:52:13Z","timestamp":1769064733000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated transcriptomic, untargeted and targeted metabolomic analyses reveal seasonal regulatory mechanisms of vascular cambium activity in woody plants: insights from Schima superba"],"prefix":"10.3389","volume":"16","author":[{"given":"Yuanhang","family":"Wu","sequence":"first","affiliation":[{"name":"College of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongguo","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingting","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Woody Oil Resources Utilisation, College of Forestry, Central South University of Forestry and Technology","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/s00468-025-02628-8","article-title":"Cambial activity in five species of the tropical dry forest: dynamics of seasonal changes in the cambial region","volume":"39","author":"Alvarado","year":"2025","journal-title":"Trees."},{"key":"B2","unstructured":"FastQC: a quality control tool for high throughput sequence data\n          \n          \n            \n              Andrews\n              S.\n            \n          \n          \n          2010"},{"key":"B3","first-page":"301","article-title":"Cambial periodicity and wood production in Acacia ehrenbergiana Hayne\u00a0growing on dry sites of Saudi Arabia","volume":"35","author":"Aref","year":"2014","journal-title":"J. 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