{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:22:27Z","timestamp":1771928547265,"version":"3.50.1"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Five advanced breeding lines of sweet potato were assessed for polyethylene glycol (PEG)-6000-mediated osmotic stress tolerance\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Significant variation among the morphophysiological properties and antioxidative enzyme activities was observed under different levels of PEG (0, 0.1, and 0.2 MPa) incorporated in Murashige and Skoog (MS) medium. An induction of antioxidative enzymes\u2014superoxide dismutase (SOD, Enzyme Commission [EC] 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.1), guaiacol peroxidase (GPX, EC 1.11.1.7), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), glutathione reductase (GR, EC 1.6.4.2), and polyphenol oxidase (PPO, EC 1.14.18.1)\u2014was observed under stress compared to the control, and this induction was pronounced in the tolerant genotypes than in the susceptible ones. Among the antioxidant enzymes, CAT showed a strong positive correlation with GPX (Pearson\u2019s correlation coefficient [\n                    <jats:italic>r<\/jats:italic>\n                    ] = 0.73), whereas MDHAR was strongly and positively correlated with APX (\n                    <jats:italic>r<\/jats:italic>\n                    = 0.73) and PPO (\n                    <jats:italic>r<\/jats:italic>\n                    = 0.68). A significant increase in antioxidative enzyme activities was associated with lower growth retardation, as evident from the correlation study. Genotypes SP\u201330, followed by SP\u201318, possessed high principal component (PC1) scores and were rich in antioxidative enzymes, whereas genotypes SP\u201324, SP\u201326, and SP\u201328 exhibited lower enzyme activities and skewed morphological traits. The overall pattern of osmotic stress tolerance among the tested advanced sweet potato breeding lines followed the order: SP\u201330 &amp;gt; SP\u201318 &amp;gt; SP\u201326 &amp;gt; SP\u201324 &amp;gt; SP\u201328. The outcome of the study encourages the advancement of SP\u201330 for inclusion in future breeding strategies and\/or its release following the official variety release procedures.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1707715","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T05:34:19Z","timestamp":1770183259000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Antioxidative enzymes as markers for the selection of advanced sweet potato breeding lines under in vitro osmotic stress conditions"],"prefix":"10.3389","volume":"17","author":[{"given":"Ananya","family":"Mishra","sequence":"first","affiliation":[{"name":"Department of Vegetable Science, College of Agriculture, Odisha University of Agriculture and Technology","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradyumna","family":"Tripathy","sequence":"additional","affiliation":[{"name":"Department of Vegetable Science, College of Agriculture, Odisha University of Agriculture and Technology","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanume Gowda","family":"Krishnappa","sequence":"additional","affiliation":[{"name":"Regional Centre, Indian Council of Agricultural Research\u2013Central Tuber Crops Research Institute (ICAR\u2013CTCRI)","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhumita","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology, Institute of Agricultural Sciences, Siksha O Anusandhan (Deemed to be University)","place":["Bhubaneswar, Odisha, India"]},{"name":"Farming System Research Centre for Hill and Plateau Region, ICAR Research Complex for Eastern Region","place":["Ranchi, Jharkhand, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sansuta","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology, Institute of Agricultural Sciences, Siksha O Anusandhan (Deemed to be University)","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satyapriya","family":"Singh","sequence":"additional","affiliation":[{"name":"Central Horticultural Experiment Station, ICAR\u2013Indian Institute of Horticultural Research","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay Bahadur Singh","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Regional Centre, Indian Council of Agricultural Research\u2013Central Tuber Crops Research Institute (ICAR\u2013CTCRI)","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rameshkumar","family":"Arutselvan","sequence":"additional","affiliation":[{"name":"Regional Centre, Indian Council of Agricultural Research\u2013Central Tuber Crops Research Institute (ICAR\u2013CTCRI)","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bibhuti Bhusan","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Regional Research and Technology Transfer Station, Odisha University of Agriculture and Technology","place":["Bhubaneswar, Odisha, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manas Ranjan","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Division of Crop Improvement, ICAR\u2013Central Tuber Crops Research Institute","place":["Thiruvananthapuram, Kerala, India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"B1","first-page":"569","volume-title":"Principles of Plant Genetics and Breeding","author":"Acquaah","year":"2007"},{"key":"B2","first-page":"273","article-title":"Catalase","volume-title":"Methods of Enzymatic Analysis","author":"Aebi","year":"1983"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1331","DOI":"10.1093\/jxb\/53.372.1331","article-title":"Role of superoxide dismutases (SODs) in controlling oxidative stress in plants","volume":"53","author":"Alscher","year":"2002","journal-title":"J. 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