{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T07:14:11Z","timestamp":1771485251233,"version":"3.50.1"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Sugarcane mosaic virus (SCMV) undermines both yield and eating quality of the chewing-cane cultivar\n                      <jats:italic>Saccharum officinarum<\/jats:italic>\n                      cv. Xuezhe. Although constant high-temperature thermotherapy can eliminate SCMV, it frequently causes serious heat injury in heat-sensitive genotypes. This study developed an alternating high-temperature thermotherapy (AHTT) scheme to eradicate SCMV while maintaining axillary-bud vigor on rooted cuttings.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A uniform-design experiment combined with quadratic regression analysis was used to optimize three factors: maximum treatment temperature (42\u201348 \u00b0C), duration of the high-temperature pulse (2\u20136 h in darkness), and the subsequent recovery phase at 38 \u00b0C (3\u20136 h under light). These cycles were applied daily over a 12-day regimen. Outcomes included sprouting, rooted-cutting performance, and virus elimination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The model predicted an optimal schedule of 47 \u00b0C for 5 h (dark) followed by a 7-h recovery at 38 \u00b0C, repeated daily for 12 days; this schedule was experimentally validated. Under the optimized regime, axillary buds on 12-day-rooted cuttings were completely SCMV-free by RT-PCR, with 97% sprouting and acceptable vigor. AHTT was also associated with increased catalase (CAT) activity and reduced peroxidase (POD) activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The model-guided AHTT protocol reconciles effective SCMV eradication with acceptable plant stress and performs better than constant-temperature thermotherapy. It provides a practical, scalable, and environmentally friendly approach to generate virus-free sugarcane planting material, with potential relevance to other vegetatively propagated, heat-sensitive crops.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1752582","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T06:42:14Z","timestamp":1771483334000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A model-guided alternating high-temperature thermotherapy achieves complete eradication of sugarcane mosaic virus and preserves physiological integrity in Saccharum officinarum cv. 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