{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T01:44:01Z","timestamp":1784598241958,"version":"3.55.0"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Iron is an essential microelement for animals, humans, and plants. Notably, approximately one-third of the world\u2019s soils are alkaline, leading to iron deficiency. Therefore, understanding the mechanism of iron absorption and transport in plants is crucial for improving iron bioavailability in crops.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this research, reverse genetics was used to identify the transcription factor MYB30 as a positive regulator of the plant response to iron deficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Discussion<\/jats:title>\n                    <jats:p>\n                      Phenotype analysis demonstrated that MYB30 mutant plants were sensitive to iron deficiency, exhibiting reduced root length, lower chlorophyll content, and elevated lipid peroxidation, whereas MYB30 overexpression lines showed enhanced tolerance. Metabolomic analysis of myb30 plant roots by mass spectrometry indicated decreased antioxidant activity and detoxification capacity under iron-deficient conditions. Interestingly, 22 metabolic pathways were altered in the\n                      <jats:italic>myb30<\/jats:italic>\n                      plant under iron deficiency. This metabolic reprogramming likely compromises plant growth. Furthermore, MYB30 reduced reactive oxygen species accumulation under iron deficiency stress by activating related genes and enhancing antioxidant enzyme activity. In summary, metabolite analysis provides detailed molecular insights into plant iron deficiency stress and supports molecular genetic breeding efforts to improve mineral nutrition in crops.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1756499","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T05:27:41Z","timestamp":1771824461000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolic analysis of MYB30 that regulates iron deficiency stress in Arabidopsis"],"prefix":"10.3389","volume":"17","author":[{"given":"Qianyuan","family":"Gong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1248\/bpb.18.1267","article-title":"Design of an antifungal methionine inhibitor not antagonized by methionine","volume":"18","author":"Aoki","year":"1995","journal-title":"Biol. Pharm. Bull."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1104\/pp.24.1.1","article-title":"Copper enzymes in isolated chloroplasts. Polyphenoloxidase in beta vulgaris","volume":"24","author":"Arnon","year":"1949","journal-title":"Plant Physiol."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.152956","article-title":"Cepharanthine: An update of its mode of action, pharmacological properties and medical applications","volume":"62","author":"Bailly","year":"2019","journal-title":"Phytomedicine."},{"key":"B4","doi-asserted-by":"publisher","first-page":"289\u2013300","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: a practical and powerful multiple testing","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Society: Ser. B (Methodologicalapproach to )"},{"key":"B5","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1093\/aob\/mcf118","article-title":"Antioxidants, oxidative damage and oxygen deprivation stress: a review","volume":"91","author":"Blokhina","year":"2003","journal-title":"Ann. botany."},{"key":"B6","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.tplants.2014.11.004","article-title":"Molecular mechanisms governing Arabidopsis iron uptake","volume":"20","author":"Brumbarova","year":"2015","journal-title":"Trends Plant science."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.134752","article-title":"Recovery mechanism of a microalgal species, Chlorella sp. from toxicity of doxylamine: Physiological and biochemical changes, and transcriptomics","volume":"474","author":"Chen","year":"2024","journal-title":"J. hazardous materials"},{"key":"B8","doi-asserted-by":"publisher","first-page":"3400","DOI":"10.1105\/tpc.104.024315","article-title":"The essential basic helix-loop-helix protein FIT1 is required for the iron deficiency response","volume":"16","author":"Colangelo","year":"2004","journal-title":"Plant Cell."},{"key":"B9","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1111\/pce.12842","article-title":"System analysis of metabolism and the transcriptome in Arabidopsis thaliana roots reveals differential co-regulation upon iron, sulfur and potassium deficiency","volume":"40","author":"Forieri","year":"2017","journal-title":"Plant Cell environment."},{"key":"B10","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/j.envpol.2016.10.041","article-title":"Phytotoxicity and uptake of roxarsone by wheat (Triticum aestivum L.) seedlings","volume":"219","author":"Fu","year":"2016","journal-title":"Environ. pollut. (Barking Essex: 1987)"},{"key":"B11","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1016\/j.plaphy.2010.08.016","article-title":"Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants","volume":"48","author":"Gill","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2023.111973","article-title":"Transcription factor MYB8 regulates iron deficiency stress response in Arabidopsis","volume":"340","author":"Gong","year":"2024","journal-title":"Plant science."},{"key":"B13","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.tplants.2004.12.004","article-title":"Nitric oxide and iron in plants: an emerging and converging story","volume":"10","author":"Graziano","year":"2005","journal-title":"Trends Plant science."},{"key":"B14","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/s0958-1669(03)00035-1","article-title":"Deciphering plant-pathogen communication: fresh perspectives for molecular resistance breeding","volume":"14","author":"Hammond-Kosack","year":"2003","journal-title":"Curr. Opin. Biotechnol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"2044","DOI":"10.1242\/dev.136432","article-title":"Phosphoinositide signaling in plant development","volume":"143","author":"Heilmann","year":"2016","journal-title":"Development."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252010952","article-title":"WRKY transcription factors in response to metal stress in plants: A review","volume":"25","author":"Huang","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1093\/mp\/ssr065","article-title":"Fitting into the harsh reality: regulation of iron-deficiency responses in dicotyledonous plants","volume":"5","author":"Ivanov","year":"2012","journal-title":"Mol. Plant"},{"key":"B18","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/j.tplants.2009.02.006","article-title":"Homing in on iron homeostasis in plants","volume":"14","author":"Jeong","year":"2009","journal-title":"Trends Plant science."},{"key":"B19","doi-asserted-by":"publisher","first-page":"19150","DOI":"10.1073\/pnas.0707010104","article-title":"The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants","volume":"104","author":"Kobayashi","year":"2007","journal-title":"Proc. Natl. Acad. Sci. United States America."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1002\/9781118060834.ch6","article-title":"Nutrition of blueberry and other calcifuges","volume":"10","author":"Korcak","year":"2011","journal-title":"Hortic. Review"},{"key":"B21","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1023\/B:RUPP.0000011300.79951.34","article-title":"The effect of root hypoxia and iron deficiency on the photosynthesis, biochemical composition, and structure of pea chloroplasts","volume":"51","author":"Ladygin","year":"2004","journal-title":"Russian J. Plant Physiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1016\/j.molp.2015.03.012","article-title":"MYB transcription factors as regulators of phenylpropanoid metabolism in plants","volume":"8","author":"Liu","year":"2015","journal-title":"Mol. Plant"},{"key":"B23","doi-asserted-by":"publisher","first-page":"7109","DOI":"10.1021\/acs.est.5b00685","article-title":"Metal-based nanotoxicity and detoxification pathways in higher plants","volume":"49","author":"Ma","year":"2015","journal-title":"Environ. Sci. technology."},{"key":"B24","doi-asserted-by":"publisher","first-page":"dev164376","DOI":"10.1242\/dev.164376","article-title":"Reactive oxygen species in plant development","volume":"145","author":"Mhamdi","year":"2018","journal-title":"Dev. (Cambridge England)"},{"key":"B25","doi-asserted-by":"publisher","first-page":"236","DOI":"10.2174\/1389201020666191015161429","article-title":"Microwave-assisted single step cinnamic acid derivatization and evaluation for cytotoxic potential","volume":"21","author":"Mishra","year":"2020","journal-title":"Curr. Pharm. Biotechnol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.tplants.2005.11.002","article-title":"Abiotic stress, the field environment and stress combination","volume":"11","author":"Mittler","year":"2006","journal-title":"Trends Plant science."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0018","article-title":"Purine and pyrimidine nucleotide synthesis and metabolism","volume":"1","author":"Moffatt","year":"2002","journal-title":"arabidopsis book"},{"key":"B28","doi-asserted-by":"publisher","first-page":"4553","DOI":"10.1021\/cr900112r","article-title":"Iron uptake and transport in plants: the good, the bad, and the ionome","volume":"109","author":"Morrissey","year":"2009","journal-title":"Chem. Rev."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2020.112289","article-title":"Specialized diterpenoid metabolism in monocot crops: Biosynthesis and chemical diversity","volume":"172","author":"Murphy","year":"2020","journal-title":"Phytochemistry."},{"key":"B30","doi-asserted-by":"publisher","first-page":"e1003953","DOI":"10.1371\/journal.pgen.1003953","article-title":"MYB10 and MYB72 are required for growth under iron-limiting conditions","volume":"9","author":"Palmer","year":"2013","journal-title":"PloS Genet."},{"key":"B31","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1093\/mp\/sst168","article-title":"Cysteine and cysteine-related signaling pathways in Arabidopsis thaliana","volume":"7","author":"Romero","year":"2014","journal-title":"Mol. Plant"},{"key":"B32","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.plaphy.2005.02.007","article-title":"Two plasma membrane H(+)-ATPase genes are differentially expressed in iron-deficient cucumber plants","volume":"43","author":"Santi","year":"2005","journal-title":"Plant Physiol. Biochem."},{"key":"B33","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1007\/s00425-002-0738-0","article-title":"Formation of transfer cells and H(+)-ATPase expression in tomato roots under P and Fe deficiency","volume":"215","author":"Schikora","year":"2002","journal-title":"Planta."},{"key":"B34","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1021\/bp990048k","article-title":"Metabolic pathway analysis: basic concepts and scientific applications in the post-genomic era","volume":"15","author":"Schilling","year":"1999","journal-title":"Biotechnol. progress."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1300","DOI":"10.1111\/j.1744-7909.2008.00761.x","article-title":"An MYB transcription factor from Malus xiaojinensis has a potential role in iron nutrition","volume":"50","author":"Shen","year":"2008","journal-title":"J. Integr. Plant Biol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1111\/j.1365-313X.2011.04565.x","article-title":"Proteasome-mediated turnover of the transcriptional activator FIT is required for plant iron-deficiency responses","volume":"66","author":"Sivitz","year":"2011","journal-title":"Plant journal: Cell Mol. Biol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1186\/s12870-022-03553-5","article-title":"MNB1 gene is involved in regulating the iron-deficiency stress response in Arabidopsis thaliana","volume":"22","author":"Song","year":"2022","journal-title":"BMC Plant Biol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1093\/mp\/sss089","article-title":"Requirement and functional redundancy of Ib subgroup bHLH proteins for iron deficiency responses and uptake in Arabidopsis thaliana","volume":"6","author":"Wang","year":"2013","journal-title":"Mol. Plant"},{"key":"B39","doi-asserted-by":"publisher","first-page":"2476","DOI":"10.1093\/pcp\/pcy168","article-title":"MdMYB58 modulates fe homeostasis by directly binding to the mdMATE43 promoter in plants","volume":"59","author":"Wang","year":"2018","journal-title":"Plant Cell Physiol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1186\/s12864-022-08311-3","article-title":"Transcriptome analysis and differential gene expression profiling of wucai (Brassica campestris L.) in response to cold stress","volume":"23","author":"Wang","year":"2022","journal-title":"BMC Genomics"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1021\/jf3039132","article-title":"Metabolite profiling of mizuna (Brassica rapa L. var. Nipponsinica) to evaluate the effects of organic matter amendments","volume":"61","author":"Watanabe","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2021.1905336","article-title":"The ell1 mutation disrupts tryptophan metabolism and induces cell death","volume":"16","author":"Xia","year":"2021","journal-title":"Plant Signaling behavior."},{"key":"B43","doi-asserted-by":"publisher","first-page":"2017","DOI":"10.1104\/pp.16.00252","article-title":"A WRKY transcription factor regulates fe translocation under fe deficiency","volume":"171","author":"Yan","year":"2016","journal-title":"Plant Physiol."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20102424","article-title":"The adaptive mechanism of plants to iron deficiency via iron uptake, transport, and homeostasis","volume":"20","author":"Zhang","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"B45","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1111\/plb.13373","article-title":"Identification of Malus halliana R2R3-MYB gene family under iron deficiency stress and functional characteristics of MhR2R3-MYB4 in Arabidopsis thaliana","volume":"24","author":"Zhang","year":"2022","journal-title":"Plant Biol. (Stuttgart Germany)"}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2026.1756499\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T05:27:42Z","timestamp":1771824462000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2026.1756499\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":45,"alternative-id":["10.3389\/fpls.2026.1756499"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2026.1756499","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,23]]},"article-number":"1756499"}}