{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:27:17Z","timestamp":1787027237583,"version":"build-2736575974"},"reference-count":75,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000040","name":"Agriculture and Agri-Food Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Non-typhoidal\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovars are enteric pathogens in humans that can be acquired from poultry products.\n                    <jats:italic>Salmonella<\/jats:italic>\n                    colonisation in poultry is an important cause of economic losses. Due to challenges in controlling\n                    <jats:italic>Salmonella<\/jats:italic>\n                    in poultry and the emergence of well-adapted, antibiotic-resistant serovars, there is a need for innovative control strategies, such as vaccines. In this work, plants were used to produce conserved antigenic epitopes of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    FepA, an outer membrane protein involved in iron uptake, genetically fused in tandem to self-assembling lumazine synthase from\n                    <jats:italic>Brucella<\/jats:italic>\n                    spp. (BLS). The recombinant proteins were purified, characterized, and their immunogenicity was assessed in chickens. Results indicated that the recombinant proteins assemble into decameric particles. These proteins elicit antigen-specific antibodies in chickens that bind to the\n                    <jats:italic>Salmonella\u2019s<\/jats:italic>\n                    cell surface. These results demonstrate that the candidate vaccine has the potential to control\n                    <jats:italic>Salmonella<\/jats:italic>\n                    colonization in poultry, helping prevent food chain contamination.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1761000","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T06:40:27Z","timestamp":1772779227000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Protein nanoparticle-based vaccine candidate produced in Nicotiana benthamiana against non-typhoidal Salmonella enterica in poultry"],"prefix":"10.3389","volume":"17","author":[{"given":"Shabnam","family":"Shamriz","sequence":"first","affiliation":[{"name":"London Research and Development Centre, Agriculture and Agri-Food Canada (AAFC)","place":["London, ON, Canada"]},{"name":"Department of Biology, University of Western Ontario","place":["London, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip H. W.","family":"Mak","sequence":"additional","affiliation":[{"name":"Guelph Research and Development Centre, AAFC","place":["Guelph, ON, Canada"]},{"name":"Department of Animal Biosciences, University of Guelph","place":["Guelph, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carly A.","family":"Charron","sequence":"additional","affiliation":[{"name":"London Research and Development Centre, Agriculture and Agri-Food Canada (AAFC)","place":["London, ON, Canada"]},{"name":"Department of Biology, University of Western Ontario","place":["London, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhua","family":"Yin","sequence":"additional","affiliation":[{"name":"Guelph Research and Development Centre, AAFC","place":["Guelph, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Garnham","sequence":"additional","affiliation":[{"name":"London Research and Development Centre, Agriculture and Agri-Food Canada (AAFC)","place":["London, ON, Canada"]},{"name":"Department of Biochemistry, University of Western Ontario","place":["London, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elijah G.","family":"Kiarie","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, University of Guelph","place":["Guelph, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moussa S.","family":"Diarra","sequence":"additional","affiliation":[{"name":"Guelph Research and Development Centre, AAFC","place":["Guelph, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rima","family":"Menassa","sequence":"additional","affiliation":[{"name":"London Research and Development Centre, Agriculture and Agri-Food Canada (AAFC)","place":["London, ON, Canada"]},{"name":"Department of Biology, University of Western Ontario","place":["London, ON, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.01170","article-title":"Expression of the multimeric and highly immunogenic brucella spp. Lumazine synthase fused to bovine rotavirus VP8d as a scaffold for antigen production in tobacco chloroplasts","volume":"6","author":"Alfano","year":"2015","journal-title":"Front. 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