{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:36:47Z","timestamp":1791329807299,"version":"4.3.1"},"reference-count":23,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88881.699110\/2022-01"],"award-info":[{"award-number":["88881.699110\/2022-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>A new cassava disease outbreak was identified in indigenous communities in Oiapoque, Amap\u00e1, Brazil, characterized by stunting, proliferation of thin shoots, broom-like leaf formations, and apical dieback. These symptoms are consistent with Cassava Witches\u2019 Broom Disease (CWBD), previously reported in other regions of South America and Asia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Metagenomic profiling, molecular diagnostics, phylogenetic analyses, and multilocus genotyping were used to investigate microbial communities associated with symptomatic cassava plants.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Rhizoctonia (Ceratobasidium) theobromae was identified as the predominant fungal species associated with symptomatic plants. Genetic analyses indicated a close relationship between Brazilian isolates and Asian reference strains, suggesting a possible transcontinental introduction and supporting an association between R. theobromae and CWBD. This represents the first confirmed report of R. theobromae in Brazil, expanding its known geographic distribution in the Americas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The detection of this quarantine pathogen represents a potential threat to cassava production, food security, and preservation of indigenous cassava landraces in Brazil. These findings reinforce the need for surveillance, phytosanitary measures, and further studies on emerging fungal pathogens associated with cassava diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1799146","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T06:20:59Z","timestamp":1779949259000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Association of Rhizoctonia theobromae with cassava witches\u2019 broom outbreak in Brazil and genetic relatedness to Southeast Asian isolates"],"prefix":"10.3389","volume":"17","author":[{"given":"Saulo Alves Santos de","family":"Oliveira","sequence":"first","affiliation":[{"name":"Embrapa Mandioca e Fruticultura, Comiss\u00e3o de Enfrentamento da Vassoura de Bruxa da Mandioca","place":["Bahia, Brazil"]},{"name":"Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Plant Virus Department","place":["Braunschweig, Niedersachsen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samar","family":"Sheat","sequence":"additional","affiliation":[{"name":"Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Plant Virus Department","place":["Braunschweig, Niedersachsen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Margaria","sequence":"additional","affiliation":[{"name":"Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Plant Virus Department","place":["Braunschweig, Niedersachsen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adilson Lopes","family":"Lima","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sul, Comiss\u00e3o de Enfrentamento da Vassoura de Bruxa da Mandioca","place":["Bag\u00e9, Rio Grande do Sul, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackson de Ara\u00fajo dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Embrapa Amap\u00e1, Macap\u00e1, Comiss\u00e3o de Enfrentamento da Vassoura de Bruxa da Mandioca","place":["Amap\u00e1, Macap\u00e1, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herm\u00ednio Souza","family":"Rocha","sequence":"additional","affiliation":[{"name":"Embrapa Pesca e Aquicultura, Comiss\u00e3o de Enfrentamento da Vassoura de Bruxa da Mandioca","place":["Palmas, Tocantins, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helton Fleck da","family":"Silveira","sequence":"additional","affiliation":[{"name":"Embrapa Mandioca e Fruticultura, Comiss\u00e3o de Enfrentamento da Vassoura de Bruxa da Mandioca","place":["Bahia, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane","family":"Ramos de Jesus","sequence":"additional","affiliation":[{"name":"Embrapa Amap\u00e1, Macap\u00e1, Comiss\u00e3o de Enfrentamento da Vassoura de Bruxa da Mandioca","place":["Amap\u00e1, Macap\u00e1, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Winter","sequence":"additional","affiliation":[{"name":"Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Plant Virus Department","place":["Braunschweig, Niedersachsen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"83","DOI":"10.11648\/j.sjac.20190704.12","article-title":"Review on nutritional value of cassava for use as a staple food","volume":"7","author":"Bayata","year":"2019","journal-title":"Sci. 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