{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T06:34:03Z","timestamp":1777358043261,"version":"3.51.4"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    DNA-binding with One Finger (Dof) transcription factors are plant-specific regulators involved in diverse biological processes, including growth, development, and stress responses. However, the Dof family in tobacco (\n                    <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    L.) remains poorly characterized. Here, we performed a genome-wide identification and comprehensive analysis of\n                    <jats:italic>Dof<\/jats:italic>\n                    genes in tobacco, revealing 75\n                    <jats:italic>NtDof<\/jats:italic>\n                    members. Through phylogenetic reconstruction, gene structure analysis, and cis-regulatory element prediction, we classified these genes into seven subfamilies and found that the CDF clade exhibits conserved exon\u2013intron structures, unique motif compositions, and enrichment of stress- and hormone-responsive cis-elements. Expression profiling showed that most\n                    <jats:italic>NtCDF<\/jats:italic>\n                    genes respond dynamically to cold and magnesium deficiency stresses. Virus-induced gene silencing (VIGS) of two representative\n                    <jats:italic>NtCDF<\/jats:italic>\n                    genes,\n                    <jats:italic>NtDof40<\/jats:italic>\n                    and\n                    <jats:italic>NtDof63<\/jats:italic>\n                    , revealed their opposing roles in cold tolerance:\n                    <jats:italic>NtDof40<\/jats:italic>\n                    acts as a positive regulator, while\n                    <jats:italic>NtDof63<\/jats:italic>\n                    functions as a negative regulator. Our findings provide a comprehensive evolutionary framework for the Dof family in tobacco and highlight the functional divergence of CDF subfamily members in abiotic stress adaptation, offering potential targets for stress-resistance breeding.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1809808","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T05:36:26Z","timestamp":1777354586000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evolutionary analysis of the Dof family in plants and identification of its members in tobacco"],"prefix":"10.3389","volume":"17","author":[{"given":"Bolun","family":"Zhi","sequence":"first","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]},{"name":"College of Agronomy, South China Agricultural University","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuebo","family":"Wang","sequence":"additional","affiliation":[{"name":"Tobacco Science Research Institute of Guangdong Province","place":["Shaoguan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanliang","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiqin","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuwen","family":"Ma","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weicai","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tobacco Science Research Institute of Guangdong Province","place":["Shaoguan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenrui","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Pan","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GAAS), Guangdong Provincial Engineering & Technology Research Center for Tobacco Breeding and Comprehensive Utilization","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1241089","article-title":"The amborella genome and the evolution of flowering plants","volume":"342","year":"2013","journal-title":"Science"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.147385","article-title":"LlLRP1, an SHI\/SRS transcription factor, mediates bulbil formation in Lilium lancifolium via regulation by LlWOX11 and response to NaCl stress","volume":"328","author":"Bai","year":"2025","journal-title":"Int. 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