{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:19:28Z","timestamp":1787552368812,"version":"build-2736575974"},"reference-count":66,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003693","name":"Korea Institute of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Ornamental hydrangeas (genus\n                    <jats:italic>Hydrangea<\/jats:italic>\n                    ) are cultivated worldwide for their diverse flower colors and attractive morphology. Here, we assembled the complete plastid genome (plastome) and 45S nuclear ribosomal DNA (45S nrDNA) sequences of 22 individuals representing\n                    <jats:italic>H. serrata<\/jats:italic>\n                    ,\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    , and related species (\n                    <jats:italic>H. arborescens<\/jats:italic>\n                    ,\n                    <jats:italic>H. paniculata<\/jats:italic>\n                    ,\n                    <jats:italic>H. petiolaris<\/jats:italic>\n                    , and\n                    <jats:italic>H. hydrangeoides<\/jats:italic>\n                    ). The plastomes contained up to 2,344 single-nucleotide polymorphisms (SNPs) and 367 insertions\/deletions (InDels) within the genus, whereas the assembled 45S nrDNA sequences showed 119 SNPs and 10 InDels. Phylogenetic analyses based on plastome and 45S nrDNA sequences clearly separated\n                    <jats:italic>H. serrata<\/jats:italic>\n                    and\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    from the other\n                    <jats:italic>Hydrangea<\/jats:italic>\n                    species. In the plastome-based tree,\n                    <jats:italic>H. petiolaris<\/jats:italic>\n                    was placed in the same clade as\n                    <jats:italic>H. arborescens<\/jats:italic>\n                    , whereas in the 45S nrDNA-based tree it showed a close relationship to\n                    <jats:italic>H. hydrangeoides<\/jats:italic>\n                    . The\n                    <jats:italic>H. serrata<\/jats:italic>\n                    and\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    samples were not always separated according to their species boundaries, as observed in samples Hse8\u2013Hse12. Notably, one\n                    <jats:italic>H. serrata<\/jats:italic>\n                    sample (Hse8), collected from a wild mountainous region of Japan, exhibited a closer genetic relationship to\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    samples, indicating that cultivated hydrangeas may have originated from a specific wild lineage of\n                    <jats:italic>H. serrata<\/jats:italic>\n                    adapted to mountainous habitats. Using plastome-derived molecular markers, 66\n                    <jats:italic>Hydrangea<\/jats:italic>\n                    samples were further classified into five groups, with Group II comprising both cultivated\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    and a subset of wild\n                    <jats:italic>H. serrata<\/jats:italic>\n                    samples, suggesting a close genetic affinity between this group and the ancestral gene pool of cultivated\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    . Based on these genomic resources, eight plastome-derived molecular markers were developed to differentiate cultivated hydrangeas from wild genotypes and to assess genetic diversity within\n                    <jats:italic>H. serrata<\/jats:italic>\n                    and\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    , providing practical tools for germplasm identification, breeding, and genetic resource management of\n                    <jats:italic>Hydrangea<\/jats:italic>\n                    species.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1836859","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T05:30:53Z","timestamp":1787549453000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic diversity, phylogenetic relationships, and marker development between Hydrangea serrata and H. macrophylla based on plastome and 45S nrDNA"],"prefix":"10.3389","volume":"17","author":[{"given":"Hyeonah","family":"Shim","sequence":"first","affiliation":[{"name":"Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University","place":["Seoul, Republic of Korea"]},{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben","place":["Seeland, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Soo","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University","place":["Chuncheon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KyoungSu","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Natural Sciences, Kyungpook National 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Espinosa","family":"Waminal","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben","place":["Seeland, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sae Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University","place":["Seoul, Republic of Korea"]},{"name":"Department of Applied Plant 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