{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:55:46Z","timestamp":1781762146812,"version":"3.54.5"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["668916"],"award-info":[{"award-number":["668916"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Drought and salinity are major constraints to wheat productivity worldwide. Heat shock protein 70 (\n                      <jats:italic>Hsp70<\/jats:italic>\n                      ) is a conserved molecular chaperone implicated in plant stress responses, but its role in cellular stability and structural adaptation in wheat remains poorly understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To investigate its function, the sorghum-derived\n                      <jats:italic>SbHsp70<\/jats:italic>\n                      gene was constitutively overexpressed in durum wheat (cv. Kofa) and bread wheat (cv. Bobwhite) via particle bombardment. Transgenic lines were evaluated under controlled drought and salinity stress conditions using physiological, cellular, molecular, and agronomic analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>SbHsp70<\/jats:italic>\n                      overexpression enhanced drought and salinity tolerance in both wheat backgrounds. Transgenic plants maintained higher membrane stability, relative water content, and photosynthetic activity under stress. Enhanced interlocking marginal lobe formation, altered actin organization, and modulation of stress-responsive gene expression were also observed. Importantly, transgenic lines maintained agronomic performance under drought without yield penalties under well-watered conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      These findings suggest that\n                      <jats:italic>SbHsp70<\/jats:italic>\n                      contributes to abiotic stress tolerance through improved cellular stability and stress-associated structural adaptations. While the observed cytoskeletal and transcriptional changes indicate a coordinated stress response, further studies are required to elucidate the underlying molecular mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1868690","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:34:44Z","timestamp":1781760884000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["SbHsp70 overexpression enhances drought and salinity tolerance in wheat through improved cellular stability and stress-associated structural adaptations"],"prefix":"10.3389","volume":"17","author":[{"given":"Sruthy Maria","family":"Augustine","sequence":"first","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayyer","family":"Abdollahi Sisi","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Scheer","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabelle","family":"Heid","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babette","family":"Knoblauch","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stjepan","family":"Vukasovic","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stavros","family":"Tzigos","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rod J","family":"Snowdon","sequence":"additional","affiliation":[{"name":"Department of Plant breeding, IFZ Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University","place":["Giessen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1007\/s12192-020-01144-7","article-title":"Central role of 70-kDa heat shock protein in adaptation of plants to drought stress","volume":"25","author":"Aghaie","year":"2020","journal-title":"Cell Stress Chaperones"},{"key":"B500","doi-asserted-by":"publisher","first-page":"503","DOI":"10.32604\/phyton.2021.013588","article-title":"Foliar Application of Phosphorus Enhances Photosynthesis and Biochemical Characteristics of Maize under Drought Stress","volume":"90","author":"Ahmad","year":"2021","journal-title":"Phyton-International Journal of Experimental Botany"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077869","article-title":"Difference in Yield and Physiological Features in Response to Drought and Salinity Combined Stress during Anthesis in Tibetan Wild and Cultivated Barleys","volume":"8","author":"Ahmed","year":"2013","journal-title":"PLoS One"},{"key":"B4","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1111\/j.1365-3040.2005.01403.x","article-title":"Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro","volume":"29","author":"Ahn","year":"2006","journal-title":"Plant Cell Environ."},{"key":"B5","first-page":"31","article-title":"Recent molecular advances to combat abiotic stress tolerance in crop plants","volume":"6","author":"Amudha","year":"2011","journal-title":"Bio\/Technol. 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