{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:47:26Z","timestamp":1785822446784,"version":"3.56.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Plants rely on photosynthesis to fix carbon and produce sugars required for growth, energy metabolism, and structural development. The roots can also absorb exogenous sugars from the surrounding soil environment. However, how acquired sugars are metabolized, regulated, and integrated into plant metabolic networks, and how this varies across species, remains poorly understood. Untargeted metabolomic profiling was applied to below-ground and aerial tissues of\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    ,\n                    <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    cv. Micro-Tom, and\n                    <jats:italic>Gossypium hirsutum<\/jats:italic>\n                    seedlings treated with \u00b9\u00b2C or \u00b9\u00b3C\n                    <jats:sub>6<\/jats:sub>\n                    -glucose (30 mM, 48\u00a0h) under continuous illumination to minimize remobilization of endogenous carbon reserves. The \u00b9\u00b2C-metabolomic profiles revealed species-specific responses among the three species examined with 40 significantly altered metabolites in\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    roots (predominantly decreased), 6 in\n                    <jats:italic>S. lycopersicum<\/jats:italic>\n                    roots (predominantly increased), and none in\n                    <jats:italic>G. hirsutum<\/jats:italic>\n                    . All three species transported \u00b9\u00b3C-glucose to aerial tissues, with\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    showing the highest \u00b9\u00b3C enrichment (0.85% dry weight), compared to\n                    <jats:italic>S. lycopersicum<\/jats:italic>\n                    cv. Micro-Tom (0.20%) and\n                    <jats:italic>G. hirsutum<\/jats:italic>\n                    (0.34%). \u00b9\u00b3C-metabolomic profiling across root, hypocotyl, and shoot tissues identified species-specific sets of labeled metabolites in\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    ,\n                    <jats:italic>S. lycopersicum<\/jats:italic>\n                    cv. Micro-Tom, and\n                    <jats:italic>G. hirsutum<\/jats:italic>\n                    . Isotopologue distributions and the degree of correlation between tissues likewise differed among the three species, indicating divergent patterns of glucose-derived carbon partitioning. These findings demonstrate that while glucose uptake is conserved among these species, its metabolic impact and utilization are different among different plant species examined.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1872531","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:38:24Z","timestamp":1785821904000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential metabolic and regulatory responses to exogenous glucose uptake across plant species revealed by isotopic metabolomics"],"prefix":"10.3389","volume":"17","author":[{"given":"Filipe","family":"Natalio","sequence":"first","affiliation":[{"name":"UCIBIO - Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, University NOVA de Lisboa, Campus da Caparica","place":["Caparica, Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1021\/acs.jproteome.9b00640","article-title":"Skyline for small molecules: a unifying software package for quantitative metabolomics","volume":"19","author":"Adams","year":"2020","journal-title":"J. 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