{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T07:45:49Z","timestamp":1785743149625,"version":"3.56.0"},"reference-count":70,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The major latex proteins (MLPs) belong to the Bet v 1\/PR-10 protein family, which is widely distributed among in higher plants and crucial for plant growth, hormone signaling, and abiotic stress responses. While\n                      <jats:italic>MLP<\/jats:italic>\n                      genes have been characterized in various plant species, the soybean\n                      <jats:italic>MLP<\/jats:italic>\n                      gene family and its contributions to salt tolerance remain poorly understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, we integrated soybean genomic and transcriptomic data to comprehensively identify\n                      <jats:italic>MLP<\/jats:italic>\n                      family members using bioinformatics approaches. We systematically analyzed their physicochemical properties, phylogenetic relationships, gene structures, collinearity, promoter cis-regulatory elements, and expression profiles under NaCl and ABA treatments. Additionally, we validated the function of the candidate gene\n                      <jats:italic>GmMLP9<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 41\n                      <jats:italic>MLP<\/jats:italic>\n                      family genes were identified in the soybean genome, with protein lengths ranging from 125 to 319 amino acids. Phylogenetic analysis revealed that soybean\n                      <jats:italic>MLP<\/jats:italic>\n                      proteins clustered into three distinct subgroups: I, II, and IV. Collinearity analysis revealed 16 pairs of collinear genes within this family, which were predominantly distributed across various chromosomes, with higher densities observed on chromosomes 7 to 9, 15, and 17, all arising from segmental duplications. Selection pressure analysis indicated that the duplicated genes primarily experienced purifying selection throughout evolution. Promoter analysis identified numerous cis-regulatory elements associated with stress and hormone responses. Expression pattern analysis demonstrated that most\n                      <jats:italic>MLP<\/jats:italic>\n                      genes exhibited elevated transcript levels in roots. Under NaCl and ABA treatments, 19 candidate\n                      <jats:italic>MLP<\/jats:italic>\n                      genes showed varying degrees of upregulation or downregulation. Furthermore, overexpression of\n                      <jats:italic>GmMLP9<\/jats:italic>\n                      significantly enhanced salt stress tolerance in both yeast and soybean plants. The soybean composite plants with\n                      <jats:italic>GmMLP9<\/jats:italic>\n                      -overexpressing hairy roots exhibited a lower Na\n                      <jats:sup>+<\/jats:sup>\n                      \/K\n                      <jats:sup>+<\/jats:sup>\n                      ratio and lower MDA content, but higher proline content.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      This study provides a theoretical foundation for further exploration of the\n                      <jats:italic>MLP<\/jats:italic>\n                      gene family\u2019s role in soybean adaptation to salt stress and offers valuable insights for identifying candidate salt-tolerance genes and advancing molecular breeding efforts\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1887220","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T07:02:21Z","timestamp":1785740541000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the major latex protein gene family and functional analysis of GmMLP9 under salt stress in soybean"],"prefix":"10.3389","volume":"17","author":[{"given":"Benshuai","family":"Yu","sequence":"first","affiliation":[{"name":"Crop Research Institute, Shandong Academy of Agricultural Sciences","place":["Jinan, Shandong, China"]},{"name":"College of Agronomy, Shandong Agricultural University","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongfan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Shandong Academy of Agricultural Sciences","place":["Jinan, Shandong, China"]},{"name":"College of 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