{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:27:50Z","timestamp":1786429670252,"version":"build-2736575974"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    The sugarcane borer (\n                    <jats:italic>Diatraea saccharalis<\/jats:italic>\n                    ) is a major pest of sugarcane in the Americas, causing significant yield and quality losses. Genetic transformation using\n                    <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                    (Bt) genes represents an effective strategy for integrated pest management. In this study,\n                    <jats:italic>cry1Ab<\/jats:italic>\n                    and\n                    <jats:italic>vip3Aa<\/jats:italic>\n                    genes were co-introduced into embryogenic calli of the commercial sugarcane variety TUC 03\u201312 using microprojectile bombardment to generate pyramided Bt events. Transgene integration was confirmed by PCR, with 247 regenerated lines testing positive for at least one transgene. Based on the total number of bombarded embryogenic calli, the overall transformation efficiency was 6.24%. Protein expression screening using lateral flow immunoassays showed that 76.6% of PCR-positive lines expressed detectable Cry1Ab protein, whereas Vip3Aa protein was detected in 33.3% of the double-positive lines. Phenotypic bioassays under controlled conditions demonstrated high larval mortality of\n                    <jats:italic>D. saccharalis<\/jats:italic>\n                    , exceeding 80% in several transgenic lines expressing both proteins. Quantification of Cry1Ab protein by ELISA revealed variability among independent lines and supported the functional relevance of protein accumulation for insect resistance. RT-qPCR analysis showed 3\u2013140-fold and 2\u20131400-fold increases in\n                    <jats:italic>cry1Ab<\/jats:italic>\n                    and\n                    <jats:italic>vip3Aa<\/jats:italic>\n                    transcript levels, respectively, compared with non-transformed plants. A strong positive correlation was observed between\n                    <jats:italic>cry1Ab<\/jats:italic>\n                    transcript levels and protein accumulation, indicating that transcriptional activity is a major determinant of toxin levels. These results demonstrate that several transgenic lines exhibit enhanced resistance to\n                    <jats:italic>D. saccharalis<\/jats:italic>\n                    under controlled conditions, including lines expressing both Cry1Ab and Vip3Aa proteins. These findings support the potential of pyramided Bt sugarcane as a promising strategy for\n                    <jats:italic>D. saccharalis<\/jats:italic>\n                    management.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1887829","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T05:35:11Z","timestamp":1786426511000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Transgenic sugarcane harboring cry1Ab and vip3Aa Bt genes for the management of Diatraea saccharalis"],"prefix":"10.3389","volume":"17","author":[{"given":"Florencia","family":"Budeguer","sequence":"first","affiliation":[{"name":"Instituto de Tecnolog\u00eda Agroindustrial del Noroeste Argentino, Estaci\u00f3n Experimental Agroindustrial Obispo Colombres - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Enrique","sequence":"additional","affiliation":[{"name":"Instituto de Tecnolog\u00eda Agroindustrial del Noroeste Argentino, Estaci\u00f3n Experimental Agroindustrial Obispo Colombres - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Garc\u00eda Degano","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Experimental Agroindustrial Obispo Colombres (EEAOC)","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Ostengo","sequence":"additional","affiliation":[{"name":"Instituto de Tecnolog\u00eda Agroindustrial del Noroeste Argentino, Estaci\u00f3n Experimental Agroindustrial Obispo Colombres - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","place":["Las Talitas, Tucum\u00e1n, Argentina"]},{"name":"Estaci\u00f3n Experimental Agroindustrial Obispo Colombres (EEAOC)","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo Sergio","family":"Noguera","sequence":"additional","affiliation":[{"name":"Instituto de Tecnolog\u00eda Agroindustrial del Noroeste Argentino, Estaci\u00f3n Experimental Agroindustrial Obispo Colombres - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","place":["Las Talitas, Tucum\u00e1n, Argentina"]},{"name":"Estaci\u00f3n Experimental Agroindustrial Obispo Colombres (EEAOC)","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Francisca","family":"Perera","sequence":"additional","affiliation":[{"name":"Instituto de Tecnolog\u00eda Agroindustrial del Noroeste Argentino, Estaci\u00f3n Experimental Agroindustrial Obispo Colombres - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefina","family":"Racedo","sequence":"additional","affiliation":[{"name":"Instituto de Tecnolog\u00eda Agroindustrial del Noroeste Argentino, Estaci\u00f3n Experimental Agroindustrial Obispo Colombres - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","place":["Las Talitas, Tucum\u00e1n, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. 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