{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:15:59Z","timestamp":1788156959369,"version":"build-2803163510"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Despite the significance of CO\n                    <jats:sub>2<\/jats:sub>\n                    as a crucial gaseous component in the soil environment, the effects of increased root-zone CO\n                    <jats:sub>2<\/jats:sub>\n                    concentrations on the physiological responses of melon and the corresponding rhizosphere microbiome are still not well understood. This study examined the effects of elevated root-zone CO\n                    <jats:sub>2<\/jats:sub>\n                    on oriental melon (Cucumis melo var. makuwa Makino) (\u2018Yu Mei Ren\u2019) in a pot experiment under controlled O\n                    <jats:sub>2<\/jats:sub>\n                    conditions. Treatments included a control group (3000 \u03bcmol\u00b7mol\n                    <jats:sup>-<\/jats:sup>\n                    \u00b9 CO\n                    <jats:sub>2<\/jats:sub>\n                    ) and three elevated concentrations: T1 (6000 \u03bcmol\u00b7mol\n                    <jats:sup>-<\/jats:sup>\n                    \u00b9), T2 (9000 \u03bcmol\u00b7mol\n                    <jats:sup>-<\/jats:sup>\n                    \u00b9), T3 (12000 \u03bcmol\u00b7mol\n                    <jats:sup>-<\/jats:sup>\n                    \u00b9). This study assessed plant growth, nutrient uptake, and rhizosphere soil properties, encompassing physicochemical characteristics and microbial community structure. The results indicated that at 10 days of treatment, T1, T2, and T3 significantly suppressed melon growth and nutrient assimilation. Additionally, a reduction in rhizosphere soil pH and an increase in electrical conductivity (EC) were noted. These differences became increasingly pronounced with extended treatment. Analysis of the rhizosphere microbial community indicated that elevated root-zone CO\n                    <jats:sub>2<\/jats:sub>\n                    decreased both diversity and richness, while simultaneously increasing the relative abundance of the phyla Acidobacteria and Actinobacteria, along with genera such as SBR1031 and A4b. These results indicate that long-term elevated root-zone CO\n                    <jats:sub>2<\/jats:sub>\n                    not only induces soil acidification and alters microbial community structure, but also impairs root nutrient acquisition.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1888248","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:41:40Z","timestamp":1788154900000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of elevated root-zone CO2 on nutrient uptake and rhizosphere microbiome in Oriental Melon (Cucumis melo var. makuwa Makino)"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaoxue","family":"He","sequence":"first","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University\/Key Laboratory of Protected Horticulture, Ministry of Education\/National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology","place":["Shenyang, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Chengde Academy of Agriculture and Forestry Sciences (Chengde AAFS)","place":["Chengde, Hebei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintong","family":"Han","sequence":"additional","affiliation":[{"name":"Suizhong County Agricultural Affairs Service Center","place":["Huludao, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenyang Institute of Technology (SIT)","place":["Shenyang, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Pei","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University\/Key Laboratory of Protected Horticulture, Ministry of Education\/National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology","place":["Shenyang, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University\/Key Laboratory of Protected Horticulture, Ministry of Education\/National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology","place":["Shenyang, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University\/Key Laboratory of Protected Horticulture, Ministry of Education\/National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology","place":["Shenyang, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiling","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University\/Key Laboratory of Protected Horticulture, Ministry of Education\/National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology","place":["Shenyang, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1111\/j.1469-8137.2004.01224.x","article-title":"What have we learned from 15 years of free\u2010air CO2 enrichment (FACE)? 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