{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:00:55Z","timestamp":1785477655957,"version":"3.56.0"},"reference-count":99,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Plant roots harbour complex microbial communities that enhance nutrient acquisition, stress tolerance, and pathogen defence, yet their assembly and functional dynamics remain incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this work, we isolated over 700 bacterial strains from wild\n                      <jats:italic>Lupinus angustifolius<\/jats:italic>\n                      across multiple compartments and soil types, capturing both dominant and rare bacterial taxa. Using co-occurrence network analysis, we selected representative strains to assemble synthetic communities (SynComs) of varying complexity, which were inoculated under sterile and non-sterile conditions. Plants were inoculated with SynComs of increasing complexity under both non-sterile soil and gnotobiotic conditions. SynCom inoculation reshaped root-associated microbiota, moderately influenced the rhizosphere, and had limited impact on bulk soil communities. Increasing SynCom complexity enhanced plant growth and triggered host transcriptional responses involving hormone signaling, defence pathways, and metabolic reprogramming.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings indicate that soil-driven filtering and microbial interactions govern microbiome assembly and plant responses. Incorporating taxa with distinct ecological roles, including low-abundance members, improves SynCom functionality and advances understanding of plant-microbe interactions in natural and agricultural systems.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1891479","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:38:59Z","timestamp":1785476339000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["From natural assemblages to synthetic communities in the Lupinus microbiome"],"prefix":"10.3389","volume":"17","author":[{"given":"Maite","family":"Ort\u00fazar","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Gen\u00e9tica, University of Salamanca","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Formariz","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Gen\u00e9tica, University of Salamanca","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhon Alexander","family":"Suesc\u00fan-Sep\u00falveda","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Gen\u00e9tica, University of Salamanca","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Gonz\u00e1lez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Gen\u00e9tica, University of Salamanca","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Riesco","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Gen\u00e9tica, University of Salamanca","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Garrido-Oter","sequence":"additional","affiliation":[{"name":"Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research","place":["Cologne, Germany"]},{"name":"Cluster of Excellence on Plant Sciences","place":["D\u00fcsseldorf, Germany"]},{"name":"Earlham Institute","place":["Norwich, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha E.","family":"Trujillo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Gen\u00e9tica, University of Salamanca","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002352","article-title":"Microbial hub taxa link host and abiotic factors to plant microbiome variation","volume":"14","author":"Agler","year":"2016","journal-title":"PloS Biol."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.02191","article-title":"A community-based culture collection for targeting novel plant growth-promoting bacteria from the sugarcane microbiome","volume":"8","author":"Armanhi","year":"2018","journal-title":"Front. 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