{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:00:43Z","timestamp":1792008043832,"version":"4.4.0"},"reference-count":82,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFF1000800"],"award-info":[{"award-number":["2024YFF1000800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32460729"],"award-info":[{"award-number":["32460729"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Efficient\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    -mediated transformation (AMT) is vital for the biotechnological improvement of sugarcane (\n                    <jats:italic>Saccharum<\/jats:italic>\n                    spp.).\n                    <jats:italic>Saccharum officinarum<\/jats:italic>\n                    is the main ancestor of all modern cultivars, yet little research has been conducted on its AMT system. In this work, an efficient AMT protocol for\n                    <jats:italic>S. officinarum<\/jats:italic>\n                    was developed, with Black Cheribon as the model genotype owing to its superior tissue culture performance and regeneration capacity. The optimized agro-infection protocol comprised the following main parameters: concentration of acetosyringone (AS) in\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    culture, concentration of AS for infection,\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    concentration at OD600\u00a0=\u00a00.4, infection time of 30 minutes, vacuum infiltration time of 10 minutes and co-cultivation time of 3 days. To further improve transformation efficiency, 0.5 mg\/L thidiazuron and 200 mg\/L citric acid were added to the regeneration medium, which enhanced the regeneration of shoots. A modified stage-dependent selection strategy (FlexII) was established by using glufosinate-ammonium at concentrations of 2.0, 1.0, and 0.75 mg\/L in the callus proliferation, shoot regeneration, and rooting stages, respectively. This strategy was more successful than the minimum inhibitory concentration-based strategy in\n                    <jats:italic>S. officinarum<\/jats:italic>\n                    transformation. The optimized protocol further boosted the transformation efficiency of Black Cheribon from 1.12% to 7.17%. The resulting transgenic lines were confirmed by PCR amplification of T-DNA regions and immunochromatographic detection of\n                    <jats:italic>Bar<\/jats:italic>\n                    protein expression in primary transformants, respectively. These results provide a sound technical foundation for the functional genomics and biotechnological optimization of\n                    <jats:italic>S. officinarum<\/jats:italic>\n                    germplasm, and may serve as a reference for future transformation studies in other sugarcane germplasm.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1893606","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:36:34Z","timestamp":1787636194000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of an efficient Agrobacterium-mediated genetic transformation protocol for Saccharum officinarum using Black Cheribon as a model genotype"],"prefix":"10.3389","volume":"17","author":[{"given":"Chunjia","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, 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Genetic Improvement","place":["Kaiyuan, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, Yunnan, China"]},{"name":"Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences\/Yunnan Key Laboratory of Sugarcane Genetic Improvement","place":["Kaiyuan, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, Yunnan, China"]},{"name":"Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences\/Yunnan Key Laboratory of Sugarcane Genetic Improvement","place":["Kaiyuan, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gul","family":"Nawaz","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, Yunnan, China"]},{"name":"Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences\/Yunnan Key Laboratory of Sugarcane Genetic Improvement","place":["Kaiyuan, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, Yunnan, China"]},{"name":"Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences\/Yunnan Key Laboratory of Sugarcane Genetic Improvement","place":["Kaiyuan, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, Yunnan, China"]},{"name":"Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences\/Yunnan Key Laboratory of Sugarcane Genetic Improvement","place":["Kaiyuan, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuebin","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crops Breeding, Yunnan Academy of Agricultural Sciences","place":["Kunming, Yunnan, China"]},{"name":"Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences\/Yunnan Key Laboratory of Sugarcane Genetic Improvement","place":["Kaiyuan, Yunnan, China"]},{"name":"Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural 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