{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:53:32Z","timestamp":1791910412343,"version":"4.3.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"\n Lonicera japonica<\/jats:italic>\n Thunb. is a traditional medicinal plant rich in bioactive flavonoids, but the transcriptional regulation of flavonoid biosynthesis remains unclear. In this study, we identified LjMYB106, an R2R3-MYB transcription factor associated with flavonoid accumulation, from floral developmental transcriptomes. Sequence and phylogenetic analyses showed that LjMYB106 contains conserved R2 and R3 MYB domains and is closely related to homologous MYB proteins. Heterologous overexpression of\n LjMYB106<\/jats:italic>\n in\n Arabidopsis thaliana<\/jats:italic>\n and\n Nicotiana benthamiana<\/jats:italic>\n increased total flavonoid and p-coumaric acid contents. Integrated transcriptomic and widely targeted metabolomic analyses in\n A. thaliana<\/jats:italic>\n showed that\n LjMYB106<\/jats:italic>\n overexpression was associated with changes in phenylpropanoid and flavonoid biosynthetic pathways. qRT-PCR validation further showed that\n PAL<\/jats:italic>\n ,\n C4H<\/jats:italic>\n ,\n 4CL<\/jats:italic>\n ,\n CHS<\/jats:italic>\n ,\n DFR<\/jats:italic>\n , and\n ANS<\/jats:italic>\n homologs were up-regulated, whereas\n CHI<\/jats:italic>\n ,\n F3H<\/jats:italic>\n , and\n FLS<\/jats:italic>\n homologs were down-regulated in\n LjMYB106<\/jats:italic>\n -overexpressing plants. Subcellular localization and yeast transactivation assays indicated that LjMYB106 is a nuclear protein with transcriptional activation activity. These results suggest that LjMYB106 is a candidate regulator of phenylpropanoid and flavonoid metabolism and provide a basis for further studies of MYB-mediated flavonoid regulation in\n L. japonica<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.3389\/fpls.2026.1896352","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:41:00Z","timestamp":1785130860000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Heterologous expression of the honeysuckle R2R3-MYB transcription factor LjMYB106 enhances phenylpropanoid and flavonoid metabolism in Arabidopsis thaliana and Nicotiana benthamiana"],"prefix":"10.3389","volume":"17","author":[{"given":"Jiangxin","family":"Yang","sequence":"first","affiliation":[{"name":"Resource Institute for Chinese and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Resource Institute for Chinese and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghua","family":"Nie","sequence":"additional","affiliation":[{"name":"Resource Institute for Chinese and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Shi","sequence":"additional","affiliation":[{"name":"Resource Institute for Chinese and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoqiao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Resource Institute for Chinese and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaotong","family":"Yang","sequence":"additional","affiliation":[{"name":"Resource Institute for Chinese and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"D444","DOI":"10.1093\/nar\/gkae1082","article-title":"InterPro: the protein sequence classification resource in 2025","volume":"53","author":"Blum","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"B2","first-page":"192","article-title":"Research progress on the regulation of flavonoid biosynthesis in medicinal plants","volume":"18","author":"Bu","year":"2021","journal-title":"Northern Pharm."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1277283","article-title":"Lonicerae Japonicae Caulis: a review of its research progress of active metabolites and pharmacological effects","volume":"14","author":"Cao","year":"2023","journal-title":"Front. 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