{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:18:36Z","timestamp":1788153516018,"version":"build-2803163510"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      ABA-aldehyde oxidase (AAO) acts as the final rate-limiting enzyme in ABA biosynthesis, and plays a critical role in plant growth and abiotic stress response. However, comprehensive analysis of\n                      <jats:italic>AAO<\/jats:italic>\n                      genes has not been reported in wheat.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, a total of 30\n                      <jats:italic>TaAAO<\/jats:italic>\n                      genes were identified, and their physicochemical properties, evolutionary relationships, gene structures,\n                      <jats:italic>cis<\/jats:italic>\n                      -acting elements, expression patterns, upstream transcription factors, interacting proteins and drought-resistant superior haplotypes were systematically analyzed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Phylogenetic analysis showed that AAO proteins were divided into five subfamilies, and TaAAOs belonged to subfamilies A, B, C and E. All TaAAOs contained MoCo and FAD domains, and TaAAOs with closer evolutionary relationships exhibited the more similar composition of conserved motifs and exon-intron structures. Promoter analysis showed that ABRE, MeJA-responsive elements, MYC and STRE elements were the most enriched. qRT-PCR assay indicated that most\n                      <jats:italic>TaAAO<\/jats:italic>\n                      genes were significantly induced by drought and salt stresses. Especially,\n                      <jats:italic>TaAAO2-A, TaAAO3-A, TaAAO4-A, TaAAO5-A<\/jats:italic>\n                      ,\n                      <jats:italic>TaAAO7-A, TaAAO8-A<\/jats:italic>\n                      and\n                      <jats:italic>TaAAO10-A<\/jats:italic>\n                      were significantly induced under both drought and salt stress. The upstream transcription factors and interacting proteins were also analyzed, and Y2H assay showed that TaAAO2-A could interact with Whirly transcription factor TaWHY1-7D. Haplotype analysis showed that\n                      <jats:italic>TaAAO2-A-Hap II<\/jats:italic>\n                      was the candidate drought-resistant haplotype with higher survival rate and expression level under drought stress.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results lay the foundation to study the function of\n                      <jats:italic>TaAAO<\/jats:italic>\n                      genes, and provide candidate genes for breeding stress-resistant wheat varieties.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1897567","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:28:51Z","timestamp":1788150531000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive analysis of ABA-aldehyde oxidase genes associated with drought and salt stress tolerance in wheat (Triticum aestivum L.)"],"prefix":"10.3389","volume":"17","author":[{"given":"Zilin","family":"Fan","sequence":"first","affiliation":[{"name":"Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation\/College of Horticulture, Ludong University","place":["Yantai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyi","family":"Ma","sequence":"additional","affiliation":[{"name":"Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation\/College of Horticulture, Ludong University","place":["Yantai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Sun","sequence":"additional","affiliation":[{"name":"Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation\/College of Horticulture, Ludong University","place":["Yantai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Lu","sequence":"additional","affiliation":[{"name":"Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation\/College of Horticulture, Ludong University","place":["Yantai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Song","sequence":"additional","affiliation":[{"name":"Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation\/College of Horticulture, Ludong University","place":["Yantai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Liu","sequence":"additional","affiliation":[{"name":"Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation\/College of Horticulture, Ludong University","place":["Yantai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1111\/pbi.14605","article-title":"A system genetics analysis uncovers the regulatory variants controlling drought response in wheat","volume":"23","author":"Chen","year":"2025","journal-title":"Plant Biotechnol. 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