{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:00:03Z","timestamp":1786719603083,"version":"build-2736575974"},"reference-count":130,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Alternative oxidase (AOX) is a core functional protein that mitigates excessive mitochondrial reactive oxygen species (ROS) accumulation and modulates plant stress adaptation, and its biological activity is tightly controlled by sophisticated regulatory networks.The cotton cytoplasmic male sterile (CMS) line Jin A represents an optimal model for dissecting the functional characteristics of\n                      <jats:italic>GhAOX<\/jats:italic>\n                      genes, as it exhibits pronounced ROS overaccumulation and aberrant\n                      <jats:italic>GhAOX<\/jats:italic>\n                      transcriptional profiles in anthers during the critical developmental stage of pollen mother cell degeneration, relative to its maintainer Jin B.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted a genome-wide identification of the\n                      <jats:italic>GhAOX<\/jats:italic>\n                      gene family in upland cotton (\n                      <jats:italic>Gossypium hirsutum<\/jats:italic>\n                      ) and systematically characterized the molecular features of\n                      <jats:italic>GhAOX<\/jats:italic>\n                      members in Jin A at the transcriptional, translational, post-translational modification, and gene structural levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of eight\n                      <jats:italic>GhAOX<\/jats:italic>\n                      genes were identified, among which\n                      <jats:italic>GhAOX2b1\/2<\/jats:italic>\n                      and\n                      <jats:italic>GhAOX2c1\/2<\/jats:italic>\n                      displayed anther-preferential expression patterns and were significantly upregulated in response to multiple abiotic stresses, including osmotic stress (PEG), salinity (NaCl), cold (4\u2103) and heat (37\u2103). Functional validation via overexpression and virus-induced gene silencing (VIGS) assays verified the ROS-scavenging capacity of\n                      <jats:italic>GhAOX<\/jats:italic>\n                      . Notably, in Jin A, despite elevated\n                      <jats:italic>GhAOX<\/jats:italic>\n                      protein abundance, its enzymatic activity was substantially compromised, which was attributed to perturbed transcriptional regulation, isoform-specific differential activation by tricarboxylic acid (TCA) cycle intermediates, and reduced abundance of mitochondrial effector molecules.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>This dysregulation was likely implicated in the aberrant ROS accumulation underlying the pollen abortion phenotype in Jin A. Collectively, this study establishes a comprehensive molecular framework for elucidating the multi-level regulatory mechanisms of AOX in response to intracellular microenvironmental perturbations and uncovers its pivotal role in regulating reproductive fitness in upland cotton.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1898123","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T14:10:31Z","timestamp":1786716631000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification of GhAOX gene family and its expression and regulation in cotton CMS line Jin A"],"prefix":"10.3389","volume":"17","author":[{"given":"Jinlong","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]},{"name":"College of Forestry, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuekai","family":"Su","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjiang","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuqing","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Forestry, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinling","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfang","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University","place":["Jinzhong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/s0014-5793(01)03261-6","article-title":"Exploring the molecular nature of alternative oxidase regulation and catalysis","volume":"510","author":"Affourtit","year":"2002","journal-title":"FEBS Lett."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-023-00380-0","article-title":"Exacerbation of drought-induced physiological and biochemical changes in leaves of Pisum sativum upon restriction of COX and AOX pathways of mitochondrial oxidative electron transport","volume":"49","author":"Analin","year":"2024","journal-title":"J. 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