{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:18:08Z","timestamp":1787807888446,"version":"build-2784847793"},"reference-count":64,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      This study comparatively evaluated heavy metal (Al, Cd, Cr, Fe, and Mn) concentrations, fatty acid composition, and palynological characteristics in\n                      <jats:italic>Hypericum scabrum<\/jats:italic>\n                      and\n                      <jats:italic>Hypericum lydium<\/jats:italic>\n                      samples collected from mining-impacted and reference sites of an iron ore operation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Plant samples of\n                      <jats:italic>H. scabrum<\/jats:italic>\n                      and\n                      <jats:italic>H. lydium<\/jats:italic>\n                      were collected from mining-impacted and reference sites in Bing\u00f6l Province, T\u00fcrkiye. Heavy metal concentrations, fatty acid composition, and pollen morphological characteristics were analyzed using ICP-MS, gas chromatography, and light microscopy, respectively. Translocation factor (TF) and statistical analyses were also performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In both species, Al, Cr, Fe, and Mn accumulation was markedly increased in the mining area, whereas the increase in Cd remained relatively limited with statistically significant differences between sites. Translocation factor (TF) analyses revealed higher values for most metals in the mining area; notably,\n                      <jats:italic>H. lydium<\/jats:italic>\n                      exhibited a greater translocation capacity for Fe and Mn. In contrast, TF values for Cr remained below 1 in both species, indicating its retention at the root level. Mining conditions were found to markedly alter the fatty acid composition in both Hypericum species, particularly affecting the balance between saturated and unsaturated fatty acids. Under heavy metal stress, total saturated fatty acids (\u03a3TSFA; particularly palmitic acid (C16:0) and stearic acid (C18:0)) increased in the mining area, whereas total unsaturated fatty acids (\u03a3TUSFA; oleic acid (C18:1), linoleic acid (C18:2), and \u03b1-linolenic acid (C18:3)) were higher under non-mining conditions. From a palynological perspective, the increased metal load preserved morphological integrity in the pollen of\n                      <jats:italic>H. scabrum<\/jats:italic>\n                      , whereas it caused pronounced changes in the P\/E ratio and pollen shape in\n                      <jats:italic>H. lydium<\/jats:italic>\n                      , with statistically significant differences observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Overall, the findings suggest that mining-related heavy metal stress influences metal accumulation, fatty acid composition, and pollen characteristics in\n                      <jats:italic>Hypericum<\/jats:italic>\n                      species, although the magnitude and nature of these effects differ between\n                      <jats:italic>H. scabrum<\/jats:italic>\n                      and\n                      <jats:italic>H. lydium<\/jats:italic>\n                      . These species-specific differences indicate that the two species exhibit distinct physiological and morphological responses to heavy metal stress, contributing to a better understanding of plant\u2013heavy metal interactions in mining ecosystems.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1900502","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:27:03Z","timestamp":1787804823000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of heavy metal contents, fatty acid composition, and palinological characteristics of two different Hypericum species growing in mining-impacted area"],"prefix":"10.3389","volume":"17","author":[{"given":"Mihriban","family":"Ah\u0131skal\u0131","sequence":"first","affiliation":[{"name":"Bing\u00f6l University Vocational School of Food, Agriculture and Livestock","place":["Bing\u00f6l, T\u00fcrkiye"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"B39","doi-asserted-by":"crossref","DOI":"10.1007\/978-94-007-4470-7","volume-title":"Heavy Metals in Soils: Trace Metals and Metalloids in Soils and Their Bioavailability","author":"Alloway","year":"2013"},{"key":"B13","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/s00425-024-04378-2","article-title":"How plants respond to heavy metal contamination: a narrative review of proteomic studies and phytoremediation applications","volume":"259","author":"Al Obaidi","year":"2024","journal-title":"Planta"},{"key":"B65","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1071\/FP23145","article-title":"Plant response to heavy metal stress toxicity: The role of metabolomics and other omics tools","volume":"50","author":"Anjitha","year":"2023","journal-title":"Funct. 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