{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T06:31:14Z","timestamp":1786343474883,"version":"build-2736575974"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Rhizoctonia<\/jats:italic>\n                      root rot has a wide host range and no resistant soybean cultivars exist, making it a serious problem.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The antifungal activity of 65 isolates of rhizobacteria against\n                      <jats:italic>R. solani<\/jats:italic>\n                      was evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Discussion<\/jats:title>\n                    <jats:p>\n                      The pathogen in the in vitro experiment was inhibited by nine rhizobacterial endophytes. The most effective rhizobacterial isolate was genus-level verified using 16S rDNA partial sequence analysis. The impact of the biocontrol isolate was investigated on\n                      <jats:italic>R. solani<\/jats:italic>\n                      infection in both greenhouse and field conditions. The rhizobacterial isolate considerably decreased the percentage of pre- and post-emergence damping-off and enhanced the plant survival compared to the untreated control grown in soil infected with\n                      <jats:italic>R. solani<\/jats:italic>\n                      .\n                      <jats:italic>Lysinibacillus fusiformis<\/jats:italic>\n                      treatment resulted in significantly stimulated antioxidant enzymes and total phenols. Transcript levels of the studied defense-related genes were considerably elevated after\n                      <jats:italic>L. fusiformis<\/jats:italic>\n                      treatment, according to quantitative RT-PCR data. Treatment of\n                      <jats:italic>L. fusiformis<\/jats:italic>\n                      in challenged plants achieved the highest levels of PR-1, PR-2, PR-10, and PR-12. The field experiments revealed that the biocontrol treatment method significantly reduced\n                      <jats:italic>Rhizoctonia<\/jats:italic>\n                      root rot incidence, stimulated plant growth, and increased yield. This research has uncovered a promising biocontrol bacterial isolate that might be used as a novel bioagent for controlling\n                      <jats:italic>R. solani<\/jats:italic>\n                      . After further toxicological and ecotoxicological evaluation, formulation development, and multi-location field trials,\n                      <jats:italic>L. fusiformis<\/jats:italic>\n                      may represent a promising candidate for development as a biocontrol agent.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1902846","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T06:18:08Z","timestamp":1786342688000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of antifungal activity of Lysinibacillus fusiformis as a biocontrol agent against Rhizoctonia solani in soybean"],"prefix":"10.3389","volume":"17","author":[{"given":"Faisal Ay","family":"Alzahrani","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Sciences and Arts, King Abdulaziz University","place":["Rabigh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehran","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Faculty of Environmental Sciences, King AbdulAziz University","place":["Jeddah, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen M.","family":"Elsharkawy","sequence":"additional","affiliation":[{"name":"Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University","place":["Kafr Elsheikh, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1556\/EuJMI.3.2013.4.7","article-title":"Bacillus thuringiensis strain 199 can induce systemic resistance in tomato against Fusarium wilt","volume":"3","author":"Akram","year":"2013","journal-title":"Eur. 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