{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:27:50Z","timestamp":1786429670244,"version":"build-2736575974"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Huanglongbing (HLB), caused by\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Liberibacter asiaticus (\n                    <jats:italic>C<\/jats:italic>\n                    Las), is a devastating citrus disease that severely threatens global citrus production, yet the metabolic mechanisms underlying citrus tolerance to HLB remain elusive. Citrus genotypes exhibit differential tolerance to HLB, with lemon (\n                    <jats:italic>Citrus limon<\/jats:italic>\n                    ) showing greater resilience compared to susceptible sweet orange (\n                    <jats:italic>Citrus sinensis<\/jats:italic>\n                    ). In this study, we integrated untargeted liquid chromatography-high resolution mass spectrometry metabolomics with a bootstrap-enhanced LASSO approach to characterize the metabolic responses of HLB-tolerant\n                    <jats:italic>C. limon<\/jats:italic>\n                    and susceptible\n                    <jats:italic>C. sinensis<\/jats:italic>\n                    to\n                    <jats:italic>C<\/jats:italic>\n                    Las infection. A total of 1,294 metabolites were identified. Bootstrap-enhanced LASSO identified stable discriminant metabolites (DMs) across four comparative frameworks, with phenylpropanoids (flavonols, furanocoumarins, and hydroxycinnamic acids) and limonoids being the most prominent DM classes. Lemon exhibited constitutively higher levels of kaempferol\/quercetin-derived flavonols, linear furanocoumarins, monolignol glycosides, and limonoids compared to orange. While\n                    <jats:italic>C<\/jats:italic>\n                    Las infection induced quantitative changes in specific metabolic branches, the overall metabolic organization remained primarily determined by genotype. In the two cultivars examined, these results suggest that pre-existing metabolic configurations distinguish the tolerant lemon from the susceptible sweet orange and are associated with their contrasting HLB responses. This study provides robust metabolic signatures and candidate pathways for improving HLB tolerance through citrus breeding programs.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1905341","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T05:35:26Z","timestamp":1786426526000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct metabolic architectures differentiate HLB-tolerant lemon and susceptible sweet orange"],"prefix":"10.3389","volume":"17","author":[{"given":"Paul","family":"Gomes","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoping","family":"Cheng","sequence":"additional","affiliation":[{"name":"Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Laffitte","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology","place":["Chengdu, China"]},{"name":"College of Ecology and Environment, Chengdu University of Technology","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Saqib","family":"Bilal","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Naveed","family":"Aslam","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur","place":["Bahawalpur, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University","place":["Fuzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiyun","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University","place":["Fuzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.plantsci.2012.07.014","article-title":"Flavonoids as antioxidants in plants: location and functional significance","volume":"196","author":"Agati","year":"2012","journal-title":"Plant Sci."},{"key":"B2","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.plaphy.2016.05.030","article-title":"Metabolic variations in different citrus rootstock cultivars associated with different responses to Huanglongbing","volume":"107","author":"Albrecht","year":"2016","journal-title":"Plant Physiol. 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