{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T14:07:47Z","timestamp":1789135667930,"version":"build-2803163510"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Soybean productivity is highly sensitive to heat and insufficient nitrogen supply, stresses that can occur simultaneously. However, organ-specific lipid responses to this stress combination remain poorly resolved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We exposed hydroponically grown soybean plants to nitrogen deficiency, heat stress (HS) and their combination and compared physiological, lipidomic and gene-expression responses in roots and leaves.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Combined stress caused the largest reductions in biomass and photosynthetic performance and the highest H\u2082O\u2082 and MDA accumulation and relative total abundance of candidate oxylipin features. Untargeted LC\u2013MS lipid profiling coupled with WGCNA revealed distinct organ-specific lipid abundance patterns. HS produced the strongest root lipidomic response, characterized by increased abundances of features putatively annotated as polyunsaturated diacylglycerols, lysophospholipids and a gibberellin A12-related compound. Leaves showed their largest lipidomic shift under combined stress, with decreased abundances of photosynthetic membrane-lipid features, increased linoleic-acid- and digalactosylmonoacylglycerol-related features and divergent changes among jasmonate-related features, including cis-jasmone. RT-qPCR showed higher\n                      <jats:italic>PLD1<\/jats:italic>\n                      and lower GA20ox expression in roots under HS. In leaves,\n                      <jats:italic>FAD2, LOX2, OPR3<\/jats:italic>\n                      and\n                      <jats:italic>MYC2<\/jats:italic>\n                      expression increased under all stress treatments, whereas PLA2 expression increased only under HS and combined stress.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings reveal pronounced tissue specificity in soybean lipid responses to combined nitrogen deficiency and HS and highlight candidate membrane-lipid- and oxylipin-associated responses for further investigation. However, they do not establish direct root\u2013shoot lipid transport or metabolic coordination.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1910848","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:22:02Z","timestamp":1789132922000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Lipidomics reveals divergent root and shoot responses to combined nitrogen deficiency and heat stress"],"prefix":"10.3389","volume":"17","author":[{"given":"Li","family":"Jia","sequence":"first","affiliation":[{"name":"Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs","place":["Guangzhou, China"]},{"name":"College of Food and Drug, Luoyang Normal University","place":["Luoyang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Huang","sequence":"additional","affiliation":[{"name":"International Research Center for Environmental Membrane Biology, School of Agricultural and Biological Engineering, Foshan University","place":["Foshan, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxin","family":"Chen","sequence":"additional","affiliation":[{"name":"International Research Center for Environmental Membrane Biology, School of Agricultural and Biological Engineering, Foshan University","place":["Foshan, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Yi","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1007\/s12298-025-01631-0","article-title":"Nitrogen deficiency identifies carbon metabolism pathways and root adaptation in maize","volume":"31","author":"Amoah","year":"2025","journal-title":"Physiol. 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