{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T15:04:35Z","timestamp":1789743875675,"version":"4.0.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Phalaenopsis-type Dendrobium<\/jats:italic>\n                      is a commercially important tropical orchid that is highly sensitive to chilling, which can cause premature leaf senescence and abscission.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We evaluated the protective effect of exogenous 24-epibrassinolide (EBR) and integrated time-resolved RNA-seq with physiological traits previously characterized in the same experimental framework. A concentration of 2.0 mg\/L was selected for transcriptomic analysis based on the refined dose-response experiment. WGCNA was performed using 18 EBR-treated libraries. Global GO and KEGG outputs were retained as descriptive overviews, whereas quantitative functional inference was based on module-specific analyses with complete multiple-testing-adjusted statistics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>EBR delayed leaf yellowing and abscission. Differential-expression analysis revealed time-dependent transcriptional responses during chilling and recovery. WGCNA identified trait-associated modules with contrasting temporal relationships to physiological traits: the pink module was positively associated with MDA and proline and negatively associated with chlorophyll, whereas the antiquewhite4 (AW4) module showed the opposite pattern. Pink-module enrichment emphasized central carbon and energy metabolism, lipid turnover, peroxisomal processes, autophagy, and cellular transport\/homeostasis. AW4 GO enrichment was dominated by transmembrane and metabolite transport; no AW4 KEGG pathway remained significant after multiple-testing correction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      These results support an association between EBR treatment and coordinated metabolic, membrane-homeostasis, and stress-response adjustments during chilling and recovery in\n                      <jats:italic>Phalaenopsis-type Dendrobium<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1912617","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:13:06Z","timestamp":1789740786000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Exogenous 24-epibrassinolide enhances chilling tolerance in Phalaenopsis-type Dendrobium through coordinated physiological and transcriptomic responses"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaoyun","family":"Yu","sequence":"first","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences","place":["Haikou, China"]},{"name":"Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture","place":["Haikou, China"]},{"name":"Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, the Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization","place":["Haikou, China"]},{"name":"National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences","place":["Sanya, 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Ministry of Agriculture","place":["Haikou, China"]},{"name":"Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, the Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization","place":["Haikou, China"]},{"name":"National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences","place":["Sanya, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangshuang","family":"Yi","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences","place":["Haikou, China"]},{"name":"Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture","place":["Haikou, China"]},{"name":"Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, the 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