{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:08:41Z","timestamp":1791335321164,"version":"4.3.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Cannabidiol (CBD), a unique secondary metabolite of\n                      <jats:italic>Cannabis sativa<\/jats:italic>\n                      L. (cannabis), is widely used in medicine, food, cosmetics, and forage. Elucidation of the transcriptional regulation mechanism can provide a theoretical basis for molecular breeding with higher CBD content. However, the regulatory network for cannabidiol biosynthesis in cannabis is largely unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Transcriptome analysis of four cannabis tissues was conducted to screen differentially expressed genes. Short time-series expression miner (STEM) analysis combined with strain-specific expression correlation analysis was performed to filter core transcription factors (TFs) associated with CBD biosynthetic enzyme genes. Yeast one-hybrid and yeast two-hybrid assays were applied to verify the binding and protein interaction relationships of key TFs, and subcellular localization assay was performed in tobacco leaf.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 1314 genes specifically upregulated in female flowers were identified by transcriptome analysis of four different tissues with distinct CBD contents and clustered into 19 profiles using short time-series expression miner (STEM) analysis. Profile 8, including 16 TFs and 6 enzyme genes related to the CBD biosynthesis pathway, demonstrated similar trends in the CBD contents of the four tissues. Among the 16 TFs, 7 exhibited similar strain-specific expression profiles and their expression levels positively correlated with those of the six enzyme genes. Moreover, a yeast one-hybrid assay found that CsMYB6 and CsMYB29 bind to the promoters of four and two CBD biosynthetic enzyme genes, respectively. Meanwhile, a yeast two-hybrid assay revealed that CsMYB6 interacts with two regulators of CBD biosynthesis, CsMYB1 and CsWRKY1, while CsMYB29 interacts with CsWRKY1. In addition, CsMYB29 and CsMYB6 were localized in the nuclei.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In summary, the two TFs, CsMYB6 and CsMYB29, identified in this study provide valuable insight into the complex regulatory mechanisms of CBD production in cannabis and offer a genetic resource for the genetic improvement of CBD content.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1914974","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:40:49Z","timestamp":1788154849000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative transcriptome analysis reveals putative transcription factors components of the regulatory network for cannabidiol biosynthesis in Cannabis sativa L"],"prefix":"10.3389","volume":"17","author":[{"given":"Ying","family":"Li","sequence":"first","affiliation":[{"name":"National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs","place":["Beijing, China"]},{"name":"Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijuan","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Li","sequence":"additional","affiliation":[{"name":"National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Cheng","sequence":"additional","affiliation":[{"name":"National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang","family":"Yang","sequence":"additional","affiliation":[{"name":"National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen","family":"Pan","sequence":"additional","affiliation":[{"name":"Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences","place":["Changsha, China"]},{"name":"Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34161","article-title":"Impact of high fat diet on long non-coding RNAs and messenger RNAs expression in the aortas of ApoE(\/) mice","volume":"6","author":"Bao","year":"2016","journal-title":"Sci. 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