{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T06:04:37Z","timestamp":1789452277672,"version":"build-2803163510"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Clubroot disease is caused by the obligate biotrophic protist\n                    <jats:italic>Plasmodiophora brassicae<\/jats:italic>\n                    and severely limits Chinese cabbage (\n                    <jats:italic>Brassica rapa<\/jats:italic>\n                    subsp.\n                    <jats:italic>pekinensis<\/jats:italic>\n                    ) production. Infection is accompanied by extensive reprogramming of host carbon metabolism and starch accumulation in enlarged roots. ADP-glucose pyrophosphorylase (AGPase) catalyzes the first committed step of starch biosynthesis, but the upstream regulation of the Chinese cabbage small-subunit gene\n                    <jats:italic>BrAGPS1<\/jats:italic>\n                    remains poorly understood. We cloned the promoter regions of\n                    <jats:italic>BrAGPS1a<\/jats:italic>\n                    (\n                    <jats:italic>BraA07g007740.3.5C<\/jats:italic>\n                    ) and\n                    <jats:italic>BrAGPS1b<\/jats:italic>\n                    (\n                    <jats:italic>BraA01g014740.3.5C<\/jats:italic>\n                    ) from the inbred line \u2018Chiifu-401-42\u2019. PlantCARE analysis predicted core promoter motifs and putative cis-regulatory elements associated with hormone, stress, and light responses. A root cDNA library prepared from 1, 3, and 5 weeks post-inoculation (wpi) was screened by yeast one-hybrid (Y1H) using selected promoter fragments as baits to recover candidate promoter-associated proteins. The screen yielded 76 nonredundant library hits, 15 of which were annotated as proteins with potential transcriptional, DNA-binding, histone-binding, or chromatin-associated functions. Integration with an RNA-sequencing (RNA-seq) time course at 1, 2, 3, and 5 wpi identified 28 Y1H-derived genes that were differentially expressed at one or more time points. Five candidates\u2014a calcium-dependent protein kinase (CPK), a basic leucine zipper (bZIP) transcription factor, a three-high-mobility-group-box (3\u00d7HMG-box) protein, and two Alfin1-like plant-homeodomain (PHD-domain) proteins\u2014were selected on the basis of functional annotation and infection-responsive expression. Quantitative real-time PCR (qRT-PCR) confirmed distinct treatment- and time-dependent expression patterns but did not test DNA binding or regulatory function. This study provides a prioritized set of\n                    <jats:italic>BrAGPS1<\/jats:italic>\n                    promoter-associated candidates and testable hypotheses for investigating the transcriptional control of starch metabolism during clubroot development. One-to-one Y1H back-assays, electrophoretic mobility shift assays, promoter transactivation tests, and genetic analyses are required to establish direct binding, regulatory direction, and biological function.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1916221","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:36:05Z","timestamp":1789450565000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Candidate promoter-associated factors of BrAGPS1 identified by yeast one-hybrid screening in Chinese cabbage during Plasmodiophora brassicae infection"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaohui","family":"Wang","sequence":"first","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taining","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuhua","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxing","family":"Pang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Shenyang Agricultural University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1038\/nature08794","article-title":"Differential innate immune signalling via Ca2+ sensor protein kinases","volume":"464","author":"Boudsocq","year":"2010","journal-title":"Nature"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1093\/jxb\/erl297","article-title":"Characterization of ADP-glucose transport across the cereal endosperm amyloplast envelope","volume":"58","author":"Bowsher","year":"2007","journal-title":"J. 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