{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T06:27:14Z","timestamp":1786602434406,"version":"build-2736575974"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Rain\u2011induced fruit cracking during sweet cherry ripening severely restricts industry development, but whether calcium signaling regulates cracking through CML genes remains unclear. Using the crack\u2011prone cultivar \u2018Brooks\u2019, we investigated the physiological and molecular mechanisms of calcium\u2011mediated regulation through exogenous CaCl\n                    <jats:sub>2<\/jats:sub>\n                    spraying, physiological index measurements, transcriptome analysis, and gene function validation. Treatment with 0.5% CaCl\n                    <jats:sub>2<\/jats:sub>\n                    was most effective, reducing the cracking rate by 53.3%, decreasing MDA content, modulating antioxidant enzyme activities, and inhibiting cell wall hydrolase activities. Transcriptome analysis identified 18 calcium\u2011signaling\u2011related DEGs, among which\n                    <jats:italic>PavCML42<\/jats:italic>\n                    showed the greatest differential expression. Tissue\u2011specific expression analysis revealed highest expression in roots and lowest in fruit. Transient overexpression of\n                    <jats:italic>PavCML42<\/jats:italic>\n                    significantly increased cell wall hydrolase activities and induced cracking, whereas VIGS silencing reduced these enzyme activities and alleviated membrane lipid peroxidation damage. Overall, exogenous calcium mitigates fruit cracking by regulating\n                    <jats:italic>PavCML42<\/jats:italic>\n                    , which is involved in cell wall metabolism and the antioxidant system, providing a candidate gene for breeding crack\u2011resistant sweet cherry cultivars.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1916674","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T05:33:02Z","timestamp":1786599182000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Regulatory effects of exogenous calcium on sweet cherry fruit cracking and functional analysis of the PavCML42 gene"],"prefix":"10.3389","volume":"17","author":[{"given":"Huijuan","family":"Yang","sequence":"first","affiliation":[{"name":"Shandong Center of Technology Innovation for Modern Protected Fruit Tree.\/Shandong institute of Pomology","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Hou","sequence":"additional","affiliation":[{"name":"Shandong Center of Technology Innovation for Modern Protected Fruit Tree.\/Shandong institute of Pomology","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaibing","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering. Shandong Agricultural University","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqin","family":"Wei","sequence":"additional","affiliation":[{"name":"Shandong Center of Technology Innovation for Modern Protected Fruit Tree.\/Shandong institute of Pomology","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junping","family":"Wu","sequence":"additional","affiliation":[{"name":"Shandong Center of Technology Innovation for Modern Protected Fruit Tree.\/Shandong institute of Pomology","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Tai\u2019an Forestry Protection and Development Center","place":["Taian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslaw","family":"Sitarek","sequence":"additional","affiliation":[{"name":"Department of Cultivars Testing, Nursery and Genetic Resources, The National Institute of Horticultural Research","place":["Skierniewice, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanjuan","family":"Fu","sequence":"additional","affiliation":[{"name":"Shandong Center of Technology Innovation for Modern Protected Fruit Tree.\/Shandong institute of Pomology","place":["Taian, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1146\/annurev.py.17.090179.000525","article-title":"Calcium-related physiological disorders of plants","volume":"17","author":"Bangerth","year":"1979","journal-title":"Annu. 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