{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:16:02Z","timestamp":1786115762759,"version":"build-2736575974"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Nucleoside diphosphate kinases (NDPKs) constitute a multifunctional enzyme family that is widely distributed in prokaryotes and eukaryotes and plays important roles in energy metabolism, stress perception, signal transduction, and reactive oxygen species homeostasis. However, this gene family has not been systematically characterized in\n                    <jats:italic>Brassica juncea.<\/jats:italic>\n                    Here, we performed a genome-wide identification and comprehensive analysis of the\n                    <jats:italic>BjNDPK<\/jats:italic>\n                    gene family by integrating bioinformatic analyses with expression and physiological assays. Eighteen non-redundant\n                    <jats:italic>BjNDPK<\/jats:italic>\n                    genes were identified and classified into five phylogenetic groups with distinct structural features. Cis-acting element analysis showed that the promoters of several members contain drought-responsive elements. Collinearity analysis indicated complex subgenomic rearrangement and selective retention during the allotetraploid evolution of\n                    <jats:italic>B. juncea<\/jats:italic>\n                    . Expression profiling showed that many\n                    <jats:italic>BjNDPK<\/jats:italic>\n                    genes differed among tissues and responded to PEG-induced osmotic stress and salt stress. Reference-gene stability analysis identified\n                    <jats:italic>qBjActin<\/jats:italic>\n                    as the most stable reference gene under the tested conditions. Physiological assays showed that PEG treatment increased the content of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    in\n                    <jats:italic>B. juncea<\/jats:italic>\n                    leaves, whereas an\n                    <jats:italic>Arabidopsis thaliana atndpk2<\/jats:italic>\n                    (corresponding to\n                    <jats:italic>BjNDPK7<\/jats:italic>\n                    in\n                    <jats:italic>B. juncea<\/jats:italic>\n                    ) mutant maintained greater root elongation than the wild type under mannitol treatment. These findings describe the structural, evolutionary, and expression characteristics of the\n                    <jats:italic>BjNDPK<\/jats:italic>\n                    family and provide candidate genes for future functional studies of abiotic-stress responses in polyploid\n                    <jats:italic>B. juncea<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1917522","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T05:31:20Z","timestamp":1785994280000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification and expression divergence of the NDPK gene family in Brassica juncea under abiotic stress"],"prefix":"10.3389","volume":"17","author":[{"given":"Huachuan","family":"He","sequence":"first","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengjia","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuming","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinwen","family":"Lei","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingdong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]},{"name":"China-Cuba Joint Natural Products Research Laboratory\/Wuhan (China-Cuba) Joint Laboratory for Natural Products under the Belt and Road Initiative","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heping","family":"Wan","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]},{"name":"China-Cuba Joint Natural Products Research Laboratory\/Wuhan (China-Cuba) Joint Laboratory for Natural Products under the Belt and Road Initiative","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jianghan University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s11816-025-00991-z","article-title":"Mitigating salt stress in cabbage: synergistic effects of nitric oxide donor GSNO and melatonin","volume":"19","author":"An","year":"2025","journal-title":"Plant Biotechnol. 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