{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:31:15Z","timestamp":1787923875448,"version":"build-2784847793"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010023","name":"Natural Science Research of Jiangsu Higher Education Institutions of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010023","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020740","name":"Natural Science Foundation of Huaian Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020740","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Methyl jasmonate (MeJA) is a potent elicitor of secondary metabolism in plants, but its regulatory role in flavonoid biosynthesis in\n                      <jats:italic>Scutellaria barbata<\/jats:italic>\n                      remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here, we performed an integrated transcriptomic and metabolomic analysis of\n                      <jats:italic>S. barbata<\/jats:italic>\n                      seedlings following exogenous MeJA treatment, combining differential expression and correlation network approaches to identify candidate genes, metabolites, and transcription factors (TFs) associated with flavonoid accumulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MeJA treatment significantly promoted the accumulation of multiple flavonoids and upregulated key biosynthetic genes, including\n                      <jats:italic>Sbar2C282T9_CHS2, Sbar5A47T51_CHI<\/jats:italic>\n                      , and\n                      <jats:italic>Sbar9A209T130_C4H<\/jats:italic>\n                      . A total of 2,453 differentially expressed genes (DEGs) were identified, which were significantly enriched in flavonoid biosynthesis and plant hormone signal transduction pathways. Among these, 180 differentially expressed TFs were detected, with the ERF, MYB, bHLH, NAC, and bZIP families being markedly enriched. Integrative correlation analysis revealed 40 TFs whose expression patterns were strongly correlated with 19 flavonoids. TF binding site prediction further identified seven candidate TFs predicted to target flavonoid structural genes, including\n                      <jats:italic>Sbar2C282T9_CHS2<\/jats:italic>\n                      and\n                      <jats:italic>Sbar9A209T130_C4H<\/jats:italic>\n                      . Protein\u2011protein interaction prediction suggested that several of these TFs, particularly bHLH members, may interact with JAZ repressors and form intra\u2011family interaction clusters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Collectively, these findings suggest a potential JA-responsive transcriptional regulatory network and point to a conserved JAZ-TF module that may modulate flavonoid biosynthesis in\n                      <jats:italic>S. barbata<\/jats:italic>\n                      . This study provides valuable insights into the regulatory mechanisms of secondary metabolism in medicinal plants and offers candidate gene resources for metabolic engineering and molecular breeding of high\u2011flavonoid varieties.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1928253","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:53:06Z","timestamp":1787921586000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated transcriptomic and metabolomic profiling reveals a JAZ-transcription factor network governing MeJA-induced flavonoid accumulation in Scutellaria barbata"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaokang","family":"Han","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Medicine, Jiangsu College of Nursing","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmin","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Jiangsu College of Nursing","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwen","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Jiangsu College of Nursing","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Jiangsu College of Nursing","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Fan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Huai\u2019an Key Laboratory of Biophysics Breeding, School of Life Science, Huaiyin Normal University","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Su","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Jiangsu College of Nursing","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyuan","family":"Man","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Huai\u2019an Key Laboratory of Biophysics Breeding, School of Life Science, Huaiyin Normal University","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Niu","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Jiangsu College of Nursing","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqing","family":"Dong","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Huai\u2019an Key Laboratory of Biophysics Breeding, School of Life Science, Huaiyin Normal University","place":["Huai\u2019an, Jiangsu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1210309","article-title":"Metabolomic and transcriptomice analyses of flavonoid biosynthesis in apricot fruits","volume":"14","author":"Chen","year":"2023","journal-title":"Front. 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