{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:22:09Z","timestamp":1788351729279,"version":"build-2803163510"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010829","name":"Xinjiang Uygur Autonomous Region Department of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010829","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Glyphosate, one of the most widely used and effective herbicides worldwide, plays a critical role in weed management. However, its application can indirectly compromise crop yield and fiber quality while controlling weeds. Consequently, breeding crop varieties that simultaneously exhibit high herbicide tolerance, high yield, and excellent quality has become a major goal in current crop breeding. To address this critical gap, we successfully developed a transgenic upland cotton line, designated as L397-1, by co-transforming the elite cultivar ZM24 with two functional genes: the herbicide-resistant\n                    <jats:italic>g10evo-epsps<\/jats:italic>\n                    and the high-yield\n                    <jats:italic>csRRM2<\/jats:italic>\n                    . Phenotypic analysis showed that L397\u20131 plants exhibited significantly enhanced tolerance to glyphosate, as well as increased yield and fiber length, indicating that this strategy effectively pyramids high-yield, high-quality, and high-herbicide-resistance traits. Furthermore, comprehensive environmental safety assessments were conducted on the transgenic plants, covering their competitive fitness in both wasteland and cultivated habitats, field arthropod community structure, incidence of major diseases, and the population density and species richness of harmful pests. The results revealed no significant differences between the transgenic L397\u20131 and its recipient ZM24 across all evaluated ecological parameters, supporting the ecological safety and application potential of L397\u20131 for future breeding utilization. In summary, this study establishes an integrated chain from \u201ctrait pyramiding technology development\u2014germplasm innovation\u2014multidimensional evaluation of breeding value\u201d, providing new insights for the synergistic improvement of multiple traits and their utilization in crop breeding.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1936824","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:03:25Z","timestamp":1788350605000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic improvement of high glyphosate resistance and high yield with environmental safety assessment"],"prefix":"10.3389","volume":"17","author":[{"given":"Peng","family":"Wang","sequence":"first","affiliation":[{"name":"Engineering Research Centre of Cotton, Ministry of Education\/College of Agriculture, Xinjiang Agricultural University","place":["Urumqi, China"]},{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]},{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production\/Cotton Research Institute of Xinjiang Academy of Agricultural Sciences","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juyun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production\/Cotton Research Institute of Xinjiang Academy of Agricultural Sciences","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadi","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Anyang High and New Zone Entrepreneurship Service Center","place":["Anyang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Anyang Institution of Sino-cotton Industry Innovation","place":["Anyang, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Ge","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]},{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production\/Cotton Research Institute of Xinjiang Academy of Agricultural Sciences","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production\/Cotton Research Institute of Xinjiang Academy of Agricultural Sciences","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanjia","family":"Chen","sequence":"additional","affiliation":[{"name":"Engineering Research Centre of Cotton, Ministry of Education\/College of Agriculture, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuguang","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Centre of Cotton, Ministry of Education\/College of Agriculture, Xinjiang Agricultural University","place":["Urumqi, China"]},{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Western Agricultural of CAAS","place":["Changji, Xinjiang, China"]},{"name":"Xinjiang Key Laboratory of Cotton Genetic Improvement and Intelligent Production\/Cotton Research Institute of Xinjiang Academy of Agricultural Sciences","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics13110971","article-title":"Glyphosate use in crop systems: risks to health and sustainable alternatives","volume":"13","author":"Aoun","year":"2025","journal-title":"Toxics"},{"key":"B2","doi-asserted-by":"crossref","DOI":"10.3390\/molecules29245965","article-title":"Environmental implication of herbicide use","volume":"29","author":"Bacmaga","year":"2024","journal-title":"Molecules"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1002\/ps.2195","article-title":"Herbicide-resistant weed management: focus on 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