{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T06:07:30Z","timestamp":1789106850360,"version":"build-2803163510"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82574543, 81173486"],"award-info":[{"award-number":["82574543, 81173486"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["252300423157, 232301420078"],"award-info":[{"award-number":["252300423157, 232301420078"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"\n Introduction<\/jats:title>\n \n Isodon rubescens<\/jats:italic>\n f. lushanensis, a typical form of\n I. rubescens<\/jats:italic>\n , is characterized by its natural deficiency in oridonin and the presence of a unique ent-kaurane diterpenoid, lushanrubescensin.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n To elucidate the molecular basis underlying the distinct chemical profiles between\n I. rubescens<\/jats:italic>\n f. lushanensis and\n I. rubescens<\/jats:italic>\n (Hemsl.) Hara, we conducted a comprehensive analysis utilizing high-quality genomic and transcriptomic data of\n I. rubescens<\/jats:italic>\n f. lushanensis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Through a genome-wide survey, our study identified 83 terpene synthase (\n TPS<\/jats:italic>\n ) genes, including 13\n TPS<\/jats:italic>\n -C and 9\n TPS-e<\/jats:italic>\n \/f subfamily genes, which are implicated in diterpenoid biosynthesis. By integrating conserved motif analysis, differential expression profiling (FPKM), and RT-qPCR validation, we successfully screened key candidate genes. Subsequent heterologous expression in\n Saccharomyces cerevisiae<\/jats:italic>\n enabled the functional characterization of five diterpene synthase genes, specifically CPS and KSL enzymes, involved in the central modules of diterpenoid synthesis.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n These findings not only expand the repertoire of known biosynthetic genes in\n I. rubescens<\/jats:italic>\n but also offer valuable insights into the divergent biosynthetic pathways of oridonin and lushanrubescensin, paving the way for future metabolic engineering and synthetic biology studies.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fpls.2026.1937259","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:30:30Z","timestamp":1789104630000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide analysis of TPS family reveals kaurene synthase-like genes in Isodon rubescens f. lushanensis"],"prefix":"10.3389","volume":"17","author":[{"given":"Hao","family":"Yang","sequence":"first","affiliation":[{"name":"College of Pharmacy, Henan Key Laboratory of Chinese Medicine Resources and Chemistry, Henan University of Chinese Medicine, Henan","place":["Zhengzhou, China"]},{"name":"Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Henan","place":["Hangzhou, China"]},{"name":"Co-Construction Collaborative Innovation Centre for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of China, Henan","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Henan Key Laboratory of Chinese Medicine Resources and Chemistry, Henan University of Chinese Medicine, Henan","place":["Zhengzhou, 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