{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:16:28Z","timestamp":1788189388343,"version":"build-2803163510"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"\n Sugar transporter proteins (STPs) play pivotal roles in hexose allocation and plant stress responses. However, systematic characterization of the STP family in tobacco (\n Nicotiana tabacum<\/jats:italic>\n ) and its involvement in\n Ralstonia solanacearum<\/jats:italic>\n resistance remains unclear. In this study, 37\n NtSTP<\/jats:italic>\n genes were identified and classified into six groups, with Group VI being the most conserved and Group V exhibiting dicot-specific expansion. Gene structure and conserved motif analyses revealed that most NtSTP members possess the typical MFS_STP domain, although variations in exon\u2013intron organization and motif composition suggested functional divergence. Tandem duplication (TD) served as the primary driver of\n NtSTP<\/jats:italic>\n family expansion, and Ka\/Ks values of all paralogous pairs were less than 1, indicative of purifying selection. Promoter cis-element analysis revealed a complex regulatory network involving hormone signaling (ABA, JA, SA, GA, ET), stress responses, and light signaling. RT-qPCR expression profiling revealed that ten\n NtSTP<\/jats:italic>\n genes (\n NtSTP1<\/jats:italic>\n ,\n 5<\/jats:italic>\n ,\n 7<\/jats:italic>\n ,\n 21<\/jats:italic>\n ,\n 22<\/jats:italic>\n ,\n 24<\/jats:italic>\n , 26,\n 27<\/jats:italic>\n ,\n 28<\/jats:italic>\n , and\n 29<\/jats:italic>\n ) exhibited significant transcriptional upregulation upon\n R. solanacearum<\/jats:italic>\n infection. Specifically,\n NtSTP5<\/jats:italic>\n ,\n NtSTP7<\/jats:italic>\n ,\n NtSTP21<\/jats:italic>\n ,\n NtSTP22<\/jats:italic>\n ,\n NtSTP24<\/jats:italic>\n ,\n NtSTP26<\/jats:italic>\n , and\n NtSTP27<\/jats:italic>\n peaked at 12\u00a0h post-inoculation (hpi), whereas\n NtSTP1<\/jats:italic>\n ,\n NtSTP28<\/jats:italic>\n , and\n NtSTP29<\/jats:italic>\n reached their highest expression levels at 24 hpi. By contrast,\n NtSTP6<\/jats:italic>\n ,\n NtSTP13<\/jats:italic>\n , and\n NtSTP30<\/jats:italic>\n displayed reduced expression upon\n R. solanacearum<\/jats:italic>\n infection. These expression patterns indicate functional diversification within the\n NtSTP<\/jats:italic>\n family and imply that these members may be transcriptionally modulated during plant responses to\n R. solanacearum<\/jats:italic>\n . The present work provides preliminary and valuable candidate gene resources that may facilitate future disease resistance breeding programs in tobacco.\n <\/jats:p>","DOI":"10.3389\/fpls.2026.1937657","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:27:21Z","timestamp":1788186441000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide characterization of the sugar transporter protein family identifies candidate genes for bacterial wilt resistance breeding in tobacco"],"prefix":"10.3389","volume":"17","author":[{"given":"Hua","family":"Xuan","sequence":"first","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University","place":["Changsha, China"]},{"name":"Yuelushan Laboratory","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University","place":["Changsha, China"]},{"name":"Yuelushan Laboratory","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renying","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University","place":["Changsha, China"]},{"name":"Yuelushan Laboratory","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingqi","family":"Yue","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University","place":["Changsha, China"]},{"name":"Yuelushan Laboratory","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University","place":["Changsha, China"]},{"name":"Yuelushan Laboratory","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guizhou Academy of Tobacco Science","place":["Guiyang, China"]},{"name":"Key Laboratory of Tobacco Biological Breeding in Mountainous Ecological Zones of China National Tobacco Corporation","place":["Guiyang, China"]},{"name":"Guizhou Provincial Key Laboratory for Tobacco Quality Improvement and Efficiency Enhancement","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University","place":["Changsha, China"]},{"name":"Yuelushan Laboratory","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushuang","family":"Guo","sequence":"additional","affiliation":[{"name":"Guizhou Academy of Tobacco Science","place":["Guiyang, China"]},{"name":"Key Laboratory of Tobacco Biological Breeding in Mountainous Ecological Zones of China National Tobacco Corporation","place":["Guiyang, China"]},{"name":"Guizhou Provincial Key Laboratory for Tobacco Quality Improvement and Efficiency Enhancement","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-245","article-title":"The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling","volume":"10","author":"Afoufa-Bastien","year":"2010","journal-title":"BMC Plant Biol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1093\/nar\/gkp335","article-title":"MEME Suite: Tools for motif discovery and searching","volume":"37","author":"Bailey","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1409","DOI":"10.1038\/s41477-021-00992-0","article-title":"Molecular mechanism of sugar transport in plants unveiled by structures of glucose\/H+ symporter STP10","volume":"7","author":"Bavnh\u00f8j","year":"2021","journal-title":"Nat. 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