{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:16:46Z","timestamp":1788419806324,"version":"build-2803163510"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The aerial parts of\n                      <jats:italic>Desmodium styracifolium<\/jats:italic>\n                      are widely used in traditional Chinese medicine to treat urinary calculi, with flavonoids as the primary active components. MYB transcription factors are key regulators of flavonoid biosynthesis in plants; however, the MYB family of\n                      <jats:italic>D. styracifolium<\/jats:italic>\n                      has not yet been systematically investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using previously obtained whole-genome and transcriptome data,\n                      <jats:italic>DsMYB<\/jats:italic>\n                      genes were identified through combined hmmsearch and blastp screening. Phylogenetic relationships were reconstructed together with 131\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                      MYB proteins. Chromosomal localization, collinearity, conserved motifs, gene structure, and promoter cis-acting elements were analyzed. Expression patterns across tissues and under high-light treatment were profiled, and eight representative genes were verified by quantitative real-time PCR (qRT-PCR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 161\n                      <jats:italic>DsMYB<\/jats:italic>\n                      genes were identified and categorized into R2R3-MYB (134), 1R-MYB (21), 3R-MYB (5), and 5R-MYB (1) subfamilies; 132 genes were unevenly distributed across nine chromosomes, with the remaining 29 located on unplaced scaffolds. Collinearity analysis indicated that segmental duplication was the primary driver of R2R3-MYB expansion. Phylogenetic analysis resolved 25 subgroups, among which the largest subgroup C13 (30 members) was specific to\n                      <jats:italic>D. styracifolium<\/jats:italic>\n                      , and subgroups C20 and C17 corresponded to the Arabidopsis anthocyanin and flavonol regulatory clades, respectively. Most DsMYB proteins shared core conserved motifs 2~5, and their promoters were enriched in light-, abscisic acid-, and methyl jasmonate-responsive elements. Eight qRT-PCR-verified\n                      <jats:italic>DsMYB<\/jats:italic>\n                      genes exhibited tissue-specific expression and distinct induction patterns under high light.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      This study provides the first systematic characterization of the MYB family in\n                      <jats:italic>D. styracifolium<\/jats:italic>\n                      . The enrichment of light- and hormone-responsive cis-elements, together with the light-responsive expression of candidate members, implicates DsMYBs in the transcriptional control of flavonoid biosynthesis, with the C17 and C20 subgroups emerging as priority candidates for flavonol and anthocyanin regulation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpls.2026.1938247","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:44:49Z","timestamp":1788417889000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification and expression analysis of the MYB transcription factor family in Desmodium styracifolium"],"prefix":"10.3389","volume":"17","author":[{"given":"Jie","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Science, Guangzhou University of Chinese Medicine","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Guangzhou University of Chinese Medicine","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Guangzhou University of Chinese Medicine","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiasheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, Guangzhou University of Chinese Medicine","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1071\/FP23269","article-title":"Functional characterisation of Dof gene family and expression analysis under abiotic stresses and melatonin-mediated tolerance in pitaya (Selenicereus undatus)","volume":"51","author":"Alam","year":"2024","journal-title":"Funct. Plant Biol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1111\/nph.15002","article-title":"Genetic analysis of the liverwort Marchantia polymorpha reveals that R2R3MYB activation of flavonoid production in response to abiotic stress is an ancient character in land plants","volume":"220","author":"Albert","year":"2018","journal-title":"New Phytol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"52","DOI":"10.2174\/2215083810666230523145738","article-title":"Phytochemical and pharmacological profile of Desmodium gangeticum (L.) DC.: A comprehensive review","volume":"10","author":"Bora","year":"2023","journal-title":"Curr. Tradit. Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1007\/s10600-018-2496-7","article-title":"New flavonol glycosides from the seeds of Desmodium styracifolium","volume":"54","author":"Cheng","year":"2018","journal-title":"Chem. Nat. Compd."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1071\/FP23261","article-title":"Genome-wide identification, phylogeny and expression analysis of the R2R3-MYB gene family in quinoa (Chenopodium quinoa) under abiotic stress","volume":"51","author":"Ding","year":"2024","journal-title":"Funct. Plant Biol."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-12-106","article-title":"Genome-wide analysis of the MYB transcription factor superfamily in soybean","volume":"12","author":"Du","year":"2012","journal-title":"BMC Plant Biol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.tplants.2010.06.005","article-title":"MYB transcription factors in Arabidopsis. [Review","volume":"15","author":"Dubos","year":"2010","journal-title":"Trends Plant Sci."},{"key":"B8","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1111\/j.1365-313X.2010.04459.x","article-title":"Evolutionary and comparative analysis of MYB and bHLH plant transcription factors","volume":"66","author":"Feller","year":"2011","journal-title":"Plant J."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.1002\/jssc.201401360","article-title":"Simultaneous quantification of 25 active constituents in the total flavonoids extract from Herba Desmodii Styracifolii by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry","volume":"38","author":"Guo","year":"2015","journal-title":"J. Sep. Sci."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1242\/dev.02801","article-title":"R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana","volume":"134","author":"Haga","year":"2007","journal-title":"Development"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/srep22980","article-title":"Genome-wide identification of R2R3-MYB genes and expression analyses during abiotic stress in Gossypium raimondii","volume":"6","author":"He","year":"2016","journal-title":"Sci. Rep."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1315","DOI":"10.1104\/pp.15.01333","article-title":"MdMYB1 regulates anthocyanin and malate accumulation by directly facilitating their transport into vacuoles in apples","volume":"170","author":"Hu","year":"2016","journal-title":"Plant Physiol."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2025.109733","article-title":"Integrative analysis of the R2R3-MYB gene family revealed that BsMYB36 and BsMYB51 significantly regulate the accumulation of flavonoids in Bletilla striata (Orchidaceae)","volume":"222","author":"Huang","year":"2025","journal-title":"Plant Physiol. Biochem."},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2020.153353","article-title":"MdMYB114 regulates anthocyanin biosynthesis and functions downstream of MdbZIP4-like in apple fruit","volume":"257","author":"Jiang","year":"2021","journal-title":"J. Plant Physiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1553","DOI":"10.11842\/wst.20231115003","article-title":"Cloning and functional characterization of a flavonoids UDP-glycosyltransferase gene DsUGT11 from Desmodium styracifolia","volume":"26","author":"Li","year":"2024","journal-title":"Mod. Tradit. Chin. Med. Mater. Med."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2019.1613131","article-title":"Research advances of MYB transcription factors in plant stress resistance and breeding","volume":"14","author":"Li","year":"2019","journal-title":"Plant Signal. Behav."},{"key":"B19","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1186\/s12870-023-04394-6","article-title":"Genomic-wide identification and expression analysis of R2R3-MYB transcription factors related to flavonol biosynthesis in Morinda officinalis","volume":"23","author":"Li","year":"2023","journal-title":"BMC Plant Biol."},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13954","article-title":"Genome-wide identification and expression analysis of MYB gene family in Cajanus cajan and CcMYB107 improves plant drought tolerance","volume":"175","author":"Li","year":"2023","journal-title":"Physiol. Plant"},{"key":"B22","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1186\/s12870-026-08555-1","article-title":"Genome-wide characterization and expression analysis of the MYB transcription factor family in mung bean (Vigna radiata)","volume":"26","author":"Li","year":"2026","journal-title":"BMC Plant Biol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ejphar.2017.10.003","article-title":"Prevention of cholesterol gallstone disease by schaftoside in lithogenic diet-induced C57BL\/6 mouse model","volume":"815","author":"Liu","year":"2017","journal-title":"Eur. J. Pharmacol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"B15","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1111\/j.1365-313X.2008.03597.x","article-title":"AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: Expression in fruit results in very high levels of both types of polyphenol","volume":"56","author":"Luo","year":"2008","journal-title":"Plant J."},{"key":"B24","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1093\/jxb\/erae192","article-title":"Interplay of light and abscisic acid signaling to modulate plant development","volume":"76","author":"Mahapatra","year":"2025","journal-title":"J. Exp. Bot."},{"key":"B25","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1016\/j.tplants.2019.07.003","article-title":"R2R3 MYB transcription factors \u2013 functions outside the DNA-binding domain","volume":"24","author":"Millard","year":"2019","journal-title":"Trends Plant Sci."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e25058","article-title":"Desmodium styracifolium: Botanical and ethnopharmacological insights, phytochemical investigations, and prospects in pharmacology and pharmacotherapy","volume":"10","author":"Opryshko","year":"2024","journal-title":"Heliyon"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/srep05018","article-title":"Expression of Arabidopsis MYB transcription factor, AtMYB111, in tobacco requires light to modulate flavonol content","volume":"4","author":"Pandey","year":"2014","journal-title":"Sci. Rep."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1515","DOI":"10.1007\/s00299-015-1803-z","article-title":"Constitutive expression of Arabidopsis MYB transcription factor, AtMYB11, in tobacco modulates flavonoid biosynthesis in favor of flavonol accumulation","volume":"34","author":"Pandey","year":"2015","journal-title":"Plant Cell Rep."},{"key":"B29","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1186\/s12864-025-11441-z","article-title":"Genome-wide identification and expression profiling of MYB transcription factors in Artemisia argyi","volume":"26","author":"Peng","year":"2025","journal-title":"BMC Genomics"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2023.1219019","article-title":"Genome-wide analysis of the R2R3-MYB gene family in Spatholobus suberectus and identification of its function in flavonoid biosynthesis","volume":"14","author":"Qin","year":"2023","journal-title":"Front. Plant Sci."},{"key":"B31","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1186\/s12864-022-08819-8","article-title":"Analysis of flavonol regulator evolution in the Brassicaceae reveals MYB12, MYB111 and MYB21 duplications and MYB11 and MYB24 gene loss","volume":"23","author":"Schilbert","year":"2022","journal-title":"BMC Genomics"},{"key":"B32","doi-asserted-by":"publisher","first-page":"6886","DOI":"10.3390\/ijms251810048","article-title":"Genome-wide identification and expression analysis of MYB transcription factor family in response to various abiotic stresses in coconut (Cocos nucifera L.)","volume":"25","author":"Si","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"B33","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1111\/j.1365-313X.2007.03078.x","article-title":"Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling","volume":"50","author":"Stracke","year":"2007","journal-title":"Plant J."},{"key":"B35","doi-asserted-by":"publisher","first-page":"4729","DOI":"10.1093\/jxb\/erae225","article-title":"A generalist regulator: MYB transcription factors regulate the biosynthesis of active compounds in medicinal plants","volume":"75","author":"Tong","year":"2024","journal-title":"J. Exp. Bot."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.134138","article-title":"A P1-like MYB transcription factor boosts biosynthesis and transport of C-glycosylated flavones in duckweed","volume":"277","author":"Wang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1186\/s12870-026-08929-5","article-title":"Genome-wide identification of the MYB gene family and identification of candidate genes that involved in anthocyanin synthesis in cowpea (Vigna unguiculata [L.] Walp)","volume":"26","author":"Xia","year":"2026","journal-title":"BMC Plant Biol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1186\/s12870-023-04290-z","article-title":"Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia","volume":"23","author":"Xie","year":"2023","journal-title":"BMC Plant Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"2106","DOI":"10.4268\/cjcmm20151108","article-title":"Comparison between Lysimachiae Herba and Desmodii styracifolii Herba in pharmacological activities","volume":"40","author":"Xiong","year":"2015","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.17304","article-title":"Genome-wide analysis of the MYB gene family in pumpkin","volume":"12","author":"Xu","year":"2024","journal-title":"PeerJ"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1023\/a:023942819106","article-title":"A maize QTL for silk maysin levels contains duplicated Myb-homologous genes which jointly regulate flavone biosynthesis","volume":"52","author":"Zhang","year":"2003","journal-title":"Plant Mol. Biol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1186\/s12864-024-10868-0","article-title":"Genome-wide identification, expression analysis of the R2R3-MYB gene family and their potential roles under cold stress in Prunus sibirica","volume":"25","author":"Zhao","year":"2024","journal-title":"BMC Genomics"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1014552","DOI":"10.3389\/fpls.2022.1000803","article-title":"Integration of ABA, GA, and light signaling in seed germination through the regulation of ABI5","volume":"13","author":"Zhao","year":"2022","journal-title":"Front. Plant Sci."},{"key":"B45","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1007\/s00299-025-03468-z","article-title":"Genome-wide identification of MYB gene family and exploration of selenium metabolism-related candidates in paper mulberry (Broussonetia papyrifera)","volume":"44","author":"Zhu","year":"2025","journal-title":"Plant Cell Rep."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2026.1938247\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:44:53Z","timestamp":1788417893000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2026.1938247\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":42,"alternative-id":["10.3389\/fpls.2026.1938247"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2026.1938247","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]},"article-number":"1938247"}}