{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:04:41Z","timestamp":1789538681609,"version":"build-2803163510"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    In response to the worsening severity of wheat stripe rust in recent years, which poses a serious threat to food security, new wheat germplasm with adult-plant resistance and superior agronomic traits is being developed. We developed five hybrid combinations by crossing Longmai479 with 50212-2, Xikemai11, Chuanmai98, Chuanyu34, and 50212-12, yielding 164 families. We subjected mixed seeds from the F\n                    <jats:sub>2<\/jats:sub>\n                    to F\n                    <jats:sub>4<\/jats:sub>\n                    generations to artificial selection, followed by manual screening for superior disease-resistant individual plants in the F\n                    <jats:sub>5<\/jats:sub>\n                    generation. In the F\n                    <jats:sub>6<\/jats:sub>\n                    generation, marker-assisted selection was performed using molecular markers tightly linked to\n                    <jats:italic>Yr10<\/jats:italic>\n                    ,\n                    <jats:italic>Yr15<\/jats:italic>\n                    ,\n                    <jats:italic>Yr17<\/jats:italic>\n                    ,\n                    <jats:italic>Yr18<\/jats:italic>\n                    ,\n                    <jats:italic>Yr26<\/jats:italic>\n                    ,\n                    <jats:italic>Yr29<\/jats:italic>\n                    , and\n                    <jats:italic>Yr30<\/jats:italic>\n                    for PCR amplification and genotyping. By combining field-based disease resistance identification with agronomic trait evaluation, wheat families exhibiting both disease resistance and superior agronomic traits, including those with single-gene or multi-gene pyramiding, were comprehensively selected. Ultimately, 18 families with disease resistance and superior agronomic traits were obtained. Among these, two families carried none of the target\n                    <jats:italic>Yr<\/jats:italic>\n                    genes, two carried one\n                    <jats:italic>Yr<\/jats:italic>\n                    gene, seven carried two\n                    <jats:italic>Yr<\/jats:italic>\n                    genes, four carried three\n                    <jats:italic>Yr<\/jats:italic>\n                    genes, and three carried four\n                    <jats:italic>Yr<\/jats:italic>\n                    genes. These new wheat germplasm lines, which exhibit disease resistance and superior agronomic traits, hold promise for development into high-quality new cultivars. They will make significant contributions to stripe rust control and wheat production by providing important genetic resources for breeding disease-resistant wheat varieties.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2026.1954529","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:31:12Z","timestamp":1789536672000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Marker-assisted breeding of new wheat germplasm with adult-plant resistance to stripe rust in the genetic background of Longmai479"],"prefix":"10.3389","volume":"17","author":[{"given":"Caihong","family":"Chen","sequence":"first","affiliation":[{"name":"Wheat Research Institute, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology","place":["Mianyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boxun","family":"Yu","sequence":"additional","affiliation":[{"name":"Wheat Research Institute, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology","place":["Mianyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyao","family":"Huang","sequence":"additional","affiliation":[{"name":"Wheat Research Institute, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology","place":["Mianyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiduo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wheat Research Institute, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology","place":["Mianyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kebing","family":"Huang","sequence":"additional","affiliation":[{"name":"Wheat Research Institute, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology","place":["Mianyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suizhuang","family":"Yang","sequence":"additional","affiliation":[{"name":"Wheat Research Institute, College of Life Sciences and Agri-Forestry, Southwest University of Science and Technology","place":["Mianyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01057","article-title":"Yellow rust epidemics worldwide were caused by pathogen races from divergent genetic lineages","volume":"8","author":"Ali","year":"2017","journal-title":"Front. 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