{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:27:26Z","timestamp":1753892846010,"version":"3.41.2"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Antibodies against SARS-CoV-2 can be detected by various testing platforms, but a detailed understanding of assay performance is critical.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> We developed and validated a simple enzyme-linked immunosorbent assay (ELISA) to detect IgG binding to the receptor-binding domain (RBD) of SARS-CoV-2, which was then applied for surveillance. ELISA results were compared to a set of complimentary serologic assays using a large panel of clinical research samples.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The RBD ELISA exhibited robust performance in ROC curve analysis (AUC&amp;gt; 0.99; Se = 89%, Sp = 99.3%). Antibodies were detected in 23\/353 (6.5%) healthcare workers, 6\/9 RT-PCR-confirmed mild COVID-19 cases, and 0\/30 non-COVID-19 cases from an ambulatory site. RBD ELISA showed a positive correlation with neutralizing activity (<jats:italic>p<\/jats:italic> = &amp;lt;0.0001, <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> = 0.26).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> We applied a validated SARS-CoV-2-specific IgG ELISA in multiple contexts and performed orthogonal testing on samples. This study demonstrates the utility of a simple serologic assay for detecting prior SARS-CoV-2 infection, particularly as a tool for efficiently testing large numbers of samples as in population surveillance. Our work also highlights that precise understanding of SARS-CoV-2 infection and immunity at the individual level, particularly with wide availability of vaccination, may be improved by orthogonal testing and\/or more complex assays such as multiplex bead assays.<\/jats:p>","DOI":"10.3389\/fpubh.2021.744535","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T12:48:41Z","timestamp":1638276521000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Application of SARS-CoV-2 Serology to Address Public Health Priorities"],"prefix":"10.3389","volume":"9","author":[{"given":"Amy 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