{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:07:52Z","timestamp":1772251672378,"version":"3.50.1"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Japanese encephalitis virus (JEV) is a mosquito-borne viral pathogen, which is becoming a growing public health concern throughout the Indo-Pacific. Five genotypes of JEV have been identified. Current vaccines are based on genotype III and provide a high degree of protection for four of the five known genotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RT-PCR, Magpix, Twist Biosciences Comprehensive Viral Research Panel (CVRP), and SISPA methods were used to detect JEV from mosquito samples collected in South Korea during 2021. These methods were compared to determine which method would be most effective for biosurveillance in the Indo-Pacific region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our data showed that RT-PCR, Twist CVRP, and SISPA methods were all able to detect JEV genotype I, however, the proprietary Magpix panel was only able to detect JEV genotype III. Use of minION sequencing for pathogen detection in arthropod samples will require further method development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Biosurveillance of vectorborne pathogens remains an area of concern throughout the Indo-Pacific. RT-PCR was the most cost effective method used in the study, but TWIST CVRP allows for the identification of over 3,100 viral genomes. Further research and comparisons will be conducted to ensure optimal methods are used for large scale biosurveillance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpubh.2022.1051754","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T04:40:01Z","timestamp":1669264801000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region"],"prefix":"10.3389","volume":"10","author":[{"given":"Gary","family":"Crispell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Zielinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Iwami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary","family":"Homas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e1231","DOI":"10.1371\/journal.pntd.0001231","article-title":"Genotype V Japanese encephalitis virus is emerging","volume":"5","author":"Li","year":"2011","journal-title":"PLoS Negl Trop Dis."},{"key":"B2","doi-asserted-by":"publisher","first-page":"9847","DOI":"10.1128\/JVI.00825-11","article-title":"Emergence of genotype I of Japanese encephalitis virus as the dominant genotype in Asia","volume":"85","author":"Pan","year":"2011","journal-title":"J Virol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"48","DOI":"10.3389\/fvets.2020.00048","article-title":"Perspectives regarding the risk of introduction of the Japanese Encephalitis Virus (JEV) in the United States","volume":"7","author":"Oliveira","year":"2020","journal-title":"Front Vet Sci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.3390\/v13061154","article-title":"Mosquito vector competence for Japanese Encephalitis Virus","volume":"13","author":"Auerswald","year":"2021","journal-title":"Viruses."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.4269\/ajtmh.2012.12-0315","article-title":"Occurrence of Japanese Encephalitis Virus mosquito vectors in relation to urban pig holdings","volume":"87","author":"Lindahl","year":"2012","journal-title":"Am Soc Trop Med Hyg."},{"key":"B6","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s13567-016-0319-z","article-title":"Japanese encephalitis virus tropism in experimentally infected pigs","volume":"47","author":"Ricklin","year":"2016","journal-title":"Vet Res."},{"key":"B7","unstructured":"Japanese Encephalitis, Japan, 2007-2016. 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