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Public Health"],"abstract":"<jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> is the causative agent of listeriosis, a highly lethal disease initiated after the ingestion of <jats:italic>Listeria<\/jats:italic>-contaminated food. This species comprises different serovars, from which 4b, 1\/2a, and 1\/2b cause most of the infections. Among the different proteins involved in pathogenesis, the internalins A (InlA) and B (InlB) are the best characterized, since they play a major role in the enterocyte entry of <jats:italic>Listeria<\/jats:italic> cells during early infection. Due to their covalent attachment to the cell wall and location on the bacterial surface, along with their exclusive presence in the pathogenic <jats:italic>L. monocytogenes<\/jats:italic>, these proteins are also used as detection targets for this species. Even though huge advancements were achieved in the enrichment steps for subsequent <jats:italic>Listeria<\/jats:italic> detection, few studies have focused on the improvement of the antibodies for immunodetection. In the present study, recombinant InlA and InlB produced in <jats:italic>Escherichia coli<\/jats:italic> were used as targets to generate antibodies via phage display using the human na\u00efve antibody libraries HAL9 and HAL10. A set of five recombinant antibodies (four against InlA, and one against InlB) were produced in scFv-Fc format and tested in indirect ELISA against a panel of 19 <jats:italic>Listeria<\/jats:italic> strains (17 species; including the three main serovars of <jats:italic>L. monocytogenes<\/jats:italic>) and 16 non-<jats:italic>Listeria<\/jats:italic> species. All five antibodies were able to recognize <jats:italic>L. monocytogenes<\/jats:italic> with 100% sensitivity (CI 29.24\u2013100.0) and specificity (CI 88.78\u2013100.0) in all three analyzed antibody concentrations. These findings show that phage display-derived antibodies can improve the biological tools to develop better immunodiagnostics for <jats:italic>L. monocytogenes<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fpubh.2022.712657","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T05:17:41Z","timestamp":1647321461000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes"],"prefix":"10.3389","volume":"10","author":[{"given":"Gustavo Mar\u00e7al Schmidt 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