{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T02:10:00Z","timestamp":1788228600739,"version":"build-2803163510"},"reference-count":80,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T00:00:00Z","timestamp":1659916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:p>A rapid and reliable method for biodosimetry of populations exposed to ionizing radiation in the event of an incident or accident is crucial for initial triage and medical attention. DNA-double strand breaks (DSBs) are indicative of radiation exposure, and DSB-repair proteins (53BP1, \u03b3H2AX, ATM, etc.) are considered sensitive markers of DSB quantification. Phospho-53BP1 and \u03b3H2AX immunofluorescence technique serves as a sensitive, reliable, and reproducible tool for the detection and quantification of DSB-repair proteins, which can be used for biological dose estimations. In this study, dose-response curves were generated for<jats:sup>60<\/jats:sup>Co-\u03b3-rays induced phospho-53 Binding Protein 1 (phospho-53BP1) foci at 1, 2, 4, 8, 16, and 24 h, post-irradiation for a dose range of 0.05\u20134 Gy using fluorescence microscopy. Following ISO recommendations, minimum detection limits (MDLs) were estimated to be 16, 18, 25, 40, 50, and 75 mGy for dose-response curves generated at 1, 2, 4, 8, 16, and 24 h post-irradiation. Colocalization and correlation of phospho-53BP1 and \u03b3H2AX were also measured in irradiated peripheral blood lymphocytes (PBLs) to gain dual confirmation. Comparative evaluation of the established curve was made by \u03b3H2AX-immunofluorescence, dicentric chromosome assay (DCA), and reciprocal translocation (RT) assays by reconstructing the dose of 6 dose-blinded samples. Coefficients of respective in-house established dose-response curves were employed to reconstruct the blind doses. Estimated doses were within the variation of 4.124%. For lower doses (0.052 Gy), phospho-53BP1 and \u03b3H2AX assays gave closer estimates with the variation of \u22124.1 to + 9% in comparison to cytogenetic assays, where variations were \u22128.5 to 24%. For higher doses (3 and 4 Gy), both the cytogenetic and immunofluorescence (phospho-53BP1 and \u03b3H2AX), assays gave comparable close estimates, with \u221211.3 to + 14.3% and \u221210.3 to \u221213.7%, variations, respectively.<\/jats:p>","DOI":"10.3389\/fpubh.2022.845200","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T05:41:36Z","timestamp":1659937296000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Establishment of in vitro Calibration Curve for 60Co-\u03b3-rays Induced Phospho-53BP1 Foci, Rapid Biodosimetry and Initial Triage, and Comparative Evaluations With \u03b3H2AX and Cytogenetic Assays"],"prefix":"10.3389","volume":"10","author":[{"given":"Rajesh Kumar","family":"Chaurasia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kapil B.","family":"Shirsath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utkarsha N.","family":"Desai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagesh N.","family":"Bhat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. K.","family":"Sapra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/s00411-014-0522-0","article-title":"Overview of the principles and practice of biodosimetry","volume":"53","author":"Swartz","year":"2014","journal-title":"Radiat Environ Biophys."},{"key":"B2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.annemergmed.2007.12.021","article-title":"Medical response to a radiologic\/nuclear event: integrated plan from the Office of the Assistant Secretary for Preparedness and Response, Department of Health and Human Services","volume":"53","author":"Coleman","year":"2009","journal-title":"Ann Emerg Med."},{"key":"B3","doi-asserted-by":"publisher","first-page":"5440","DOI":"10.1182\/blood-2008-01-134817","article-title":"Radiologic and nuclear events: contingency planning for hematologists\/oncologists","volume":"111","author":"Weinstock","year":"2008","journal-title":"Blood."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.7326\/0003-4819-140-12-200406150-00015","article-title":"Medical management of the acute radiation syndrome: recommendations of the Strategic National Stockpile Radiation Working Group","volume":"140","author":"Waselenko","year":"2004","journal-title":"Ann Intern Med."},{"key":"B5","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1089\/hs.2017.0050","article-title":"Biodosimetry: a future tool for medical management of radiological emergencies","volume":"15","author":"Sproull","year":"2017","journal-title":"Health Security."},{"key":"B6","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1667\/RR3231.1","article-title":"Comparison of established and emerging biodosimetry assays","volume":"180","author":"Rothkamm","year":"2013","journal-title":"Radiat Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/S0027-5107(00)00172-X","article-title":"In vitro transmission of chromosomal aberrations through mitosis in human lymphocytes","volume":"474","author":"Pala","year":"2001","journal-title":"Mut Res."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-017-03198-7","article-title":"Transmission of induced chromosomal aberrations through successive mitotic divisions in human lymphocytes after in vitro and in vivo radiation","volume":"7","author":"Kaddour","year":"2017","journal-title":"Sci Rep."},{"key":"B9","article-title":"Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies. IAEA International Atomic Energy Agency. - Ser","author":"Ainsbury","year":"2011"},{"key":"B10","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1093\/rpd\/ncq499","article-title":"Review of retrospective dosimetry techniques for external ionising radiation exposures","volume":"147","author":"Ainsbury","year":"2011","journal-title":"Radiat Prot Dosimetry."},{"key":"B11","doi-asserted-by":"publisher","first-page":"95","DOI":"10.3390\/radiation1020009","article-title":"Use of biological dosimetry for monitoring medical workers occupationally exposed to ionizing radiation","volume":"1","author":"Piotrowski","year":"2021","journal-title":"Radiation."},{"key":"B12","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1038\/85798","article-title":"DNA double-strand breaks: signaling, repair and the cancer connection","volume":"27","author":"Khanna","year":"2001","journal-title":"Nat Genet."},{"key":"B13","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/S1383-5742(99)00042-3","article-title":"Repair and consequences of double-strand breaks in DNA","volume":"428","author":"Pastink","year":"1999","journal-title":"Mut Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.1083\/jcb.151.7.1381","article-title":"p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks","volume":"151","author":"Schultz","year":"2000","journal-title":"J Cell Biol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"2708","DOI":"10.1074\/jbc.M007665200","article-title":"Negative cell cycle regulation and DNA damage-inducible phosphorylation of the BRCT protein 53BP1","volume":"276","author":"Xia","year":"2001","journal-title":"J Biol Chem."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1719","DOI":"10.1128\/MCB.21.5.1719-1729.2001","article-title":"Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damage","volume":"21","author":"Anderson","year":"2001","journal-title":"Mol Cell Biol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1083\/jcb.153.3.613","article-title":"Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage\u2013signaling pathways","volume":"153","author":"Rappold","year":"2001","journal-title":"J Cell Biol."},{"key":"B18","first-page":"8586","article-title":"53BP1 and NFBD1\/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage","volume":"63","author":"Mochan","year":"2003","journal-title":"Cancer Res."},{"key":"B19","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1083\/jcb.200510130","article-title":"Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks","volume":"173","author":"Bekker-Jensen","year":"2006","journal-title":"J Cell Biol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1038\/nature04384","article-title":"A phosphatase complex that dephosphorylates \u03b3H2AX regulates DNA damage checkpoint recovery","volume":"439","author":"Keogh","year":"2006","journal-title":"Nature."},{"key":"B21","doi-asserted-by":"publisher","first-page":"56617","DOI":"10.3791\/56617","article-title":"Immunofluorescence microscopy of \u03b3H2AX and 53BP1 for analyzing the formation and repair of DNA double-strand breaks","volume":"129","author":"Popp","year":"2017","journal-title":"J Visualized Exp."},{"key":"B22","doi-asserted-by":"publisher","first-page":"zcab046","DOI":"10.1093\/narcan\/zcab046","article-title":"Quantification of radiation-induced DNA double strand break repair foci to evaluate and predict biological responses to ionizing radiation. NAR Cancer","volume":"3","author":"Penninckx","year":"2021"},{"key":"B23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12885-015-1890-9","article-title":"A prospective study on histone \u03b3-H2AX and 53BP1 foci expression in rectal carcinoma patients: correlation with radiation therapy-induced outcome","volume":"15","author":"Djuzenova","year":"2015","journal-title":"BMC Cancer."},{"key":"B24","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/s00411-014-0514-0","article-title":"The RABiT: high-throughput technology for assessing global DSB repair","volume":"53","author":"Turner","year":"2014","journal-title":"Radiat Environ Biophys."},{"key":"B25","doi-asserted-by":"publisher","first-page":"108350","DOI":"10.1016\/j.mrrev.2020.108350","article-title":"Potential application of \u03b3-H2AX as a biodosimetry tool for radiation triage","volume":"787","author":"Raavi","year":"2021","journal-title":"Mutat Res"},{"key":"B26","first-page":"8","article-title":"\u201cClinical applications of biological dosimetry in patients exposed to low dose radiation due to radiological, imaging or nuclear medicine procedures,\u201d in Seminars in Nuclear Medicine","author":"Vinnikov","year":"2021"},{"key":"B27","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1667\/RR13911.1","article-title":"Biodosimetry based on \u03b3-H2AX quantification and cytogenetics after partial-and total-body irradiation during fractionated radiotherapy","volume":"183","author":"Zahnreich","year":"2015","journal-title":"Radiat Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1667\/RR1775.1","article-title":"Quantification of \u03b3-H2AX foci in human lymphocytes: a method for biological dosimetry after ionizing radiation exposure","volume":"174","author":"Roch-Lef\u00e8vre","year":"2010","journal-title":"Radiat Res."},{"key":"B29","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1667\/RR3486.1","article-title":"Computational methods for analysis of foci: validation for radiation-induced \u03b3-H2AX foci in human cells","volume":"165","author":"B\u00f6cker","year":"2006","journal-title":"Radiat Res."},{"key":"B30","first-page":"2014","volume-title":"Radiation Protection\u2013Performance Criteria for Service Laboratories Performing Biological Dosimetry by Cytogenetics","year":"2004"},{"key":"B31","first-page":"932","article-title":"Manual of Clinical Immunology","author":"Rose","year":"1976"},{"key":"B32","doi-asserted-by":"publisher","first-page":"503354","DOI":"10.1016\/j.mrgentox.2021.503354","article-title":"Establishment and multiparametric-cytogenetic validation of 60Co-gamma-ray induced, phospho-gamma-H2AX calibration curve for rapid biodosimetry and triage management during radiological emergencies","volume":"866","author":"Chaurasia","year":"2021","journal-title":"Mutat Res"},{"key":"B33","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.mrgentox.2014.08.007","article-title":"Cyto-genotoxicity assessment of potential radioprotector. 3, 3\u2032-diselenodipropionic acid (DSePA) in Chinese Hamster Ovary (CHO) cells and human peripheral blood lymphocytes","volume":"774","author":"Chaurasia","year":"2014","journal-title":"Mutat Res"},{"key":"B34","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/0027-5107(91)90130-G","article-title":"Radiation-induced chromosomal breakage and rejoining in interphase-metaphase chromosomes of human lymphocytes","volume":"249","author":"Vyas","year":"1991","journal-title":"Mutat Res."},{"key":"B35","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/ncb2941","article-title":"The biogenesis of chromosome translocations","volume":"16","author":"Roukos","year":"2014","journal-title":"Nat Cell Biol."},{"key":"B36","first-page":"536","article-title":"Dose rate effect and its implications in biodosimetry using chromosomal aberration analysis","volume":"26","author":"Bhat","year":"2003","journal-title":"Radiat Protect Environ."},{"key":"B37","volume-title":"Radiation Protection\u2013Performance Criteria for Laboratories Performing Cytogenetic Triage for Assessment of Mass Casualties in Radiological or Nuclear Emergencies\u2013General Principles and Application to Dicentric Assay","year":"2008"},{"key":"B38","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1093\/mutage\/geh031","article-title":"Transmission of \u03b3-ray-induced unstable chromosomal aberrations through successive mitotic divisions in human lymphocytes in vitro","volume":"19","author":"Krishnaja","year":"2004","journal-title":"Mutagenesis."},{"key":"B39","doi-asserted-by":"publisher","first-page":"100834","DOI":"10.1016\/j.xpro.2021.100834","article-title":"Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature","volume":"2","author":"Chaurasia","year":"2021","journal-title":"STAR Protocols"},{"key":"B40","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1093\/mutage\/15.4.303","article-title":"Spontaneous and X-ray-induced chromosomal aberrations in Werner syndrome cells detected by FISH using chromosome-specific painting probes","volume":"15","author":"Grigorova","year":"2000","journal-title":"Mutagenesis."},{"key":"B41","volume-title":"Radiological Protection\u2013Performance Criteria for Laboratories Using Fluorescence in situ Hybridization (FISH) Translocation Assay for Assessment of Exposure to Ionizing Radiation","year":"2019"},{"key":"B42","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1007\/s00066-020-01576-1","article-title":"Assessment of DNA damage by 53PB1 and pKu70 detection in peripheral blood lymphocytes by immunofluorescence and high-resolution transmission electron microscopy","volume":"196","author":"Lorat","year":"2020","journal-title":"Strahlentherapie und Onkologie."},{"key":"B43","doi-asserted-by":"publisher","first-page":"58","DOI":"10.3390\/genes11010058","article-title":"Biodosimetry of low dose ionizing radiation using DNA repair foci in human lymphocytes","volume":"11","author":"Jakl","year":"2020","journal-title":"Genes."},{"key":"B44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep38295","article-title":"\u03b3-H2AX\/53BP1\/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes","volume":"6","author":"Ding","year":"2016","journal-title":"Sci Rep."},{"key":"B45","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1016\/j.radmeas.2011.02.001","article-title":"Candidate protein biomarkers as rapid indicators of radiation exposure","volume":"46","author":"Horn","year":"2011","journal-title":"Radiat Measur."},{"key":"B46","doi-asserted-by":"publisher","first-page":"e0147968","DOI":"10.1371\/journal.pone.0147968","article-title":"Analysis of lymphocytic DNA damage in early multiple sclerosis by automated gamma-H2AX and 53BP1 foci detection: a case control study","volume":"11","author":"Rasche","year":"2016","journal-title":"PLoS ONE"},{"key":"B47","doi-asserted-by":"publisher","first-page":"e0123174","DOI":"10.1371\/journal.pone.0123174","article-title":"Calibration of the \u03b3-H2AX DNA double strand break focus assay for internal radiation exposure of blood lymphocytes","volume":"10","author":"Eberlein","year":"2015","journal-title":"PLoS ONE"},{"key":"B48","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1080\/09553002.2020.1793024","article-title":"Comparison of inexperienced operators and experts in \u03b3H2A","volume":"96","author":"Bucher","year":"2020","journal-title":"X and 53BP1 foci assay for high-throughput biodosimetry approaches in a mass casualty incident. Int J Radiat Biol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/jcp.25048","article-title":"Mechanisms and consequences of double-strand DNA break formation in chromatin","volume":"231","author":"Cannan","year":"2016","journal-title":"J Cell Physiol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1016\/j.cell.2017.01.002","article-title":"Endogenous DNA damage as a source of genomic instability in cancer","volume":"168","author":"Tubbs","year":"2017","journal-title":"Cell."},{"key":"B51","doi-asserted-by":"publisher","first-page":"25377","DOI":"10.18632\/oncotarget.8298","volume":"7","author":"Sharma","year":"2016","journal-title":"Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations. Oncotarget."},{"key":"B52","doi-asserted-by":"publisher","first-page":"24097","DOI":"10.1074\/jbc.272.39.24097","article-title":"Double strand break repair","volume":"272","author":"Chu","year":"1997","journal-title":"J Biol Chem."},{"key":"B53","doi-asserted-by":"crossref","first-page":"4139","DOI":"10.4049\/jimmunol.159.9.4139","article-title":"Ig Sgamma3 DNA-specifc double strand breaks are induced in mitogen-activated B cells and are implicated in switch recombination","volume":"159","author":"Wuerffel","year":"1997","journal-title":"J Immunol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"e25113","DOI":"10.1371\/journal.pone.0025113","volume":"6","author":"Horn","year":"2011","journal-title":"Gamma-H2AX-based dose estimation for whole and partial body radiation exposure. PLoS ONE."},{"key":"B55","volume-title":"Effects of lamin A\/C structure on 53BP1 foci kinetics","author":"Lehn","year":"2021"},{"key":"B56","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-020-78354-7","article-title":"Impact of hypoxia on the double-strand break repair after photon and carbon ion irradiation of radioresistant HNSCC cells","volume":"10","author":"Wozny","year":"2020","journal-title":"Sci Rep."},{"key":"B57","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1007\/s00411-020-00852-z","article-title":"Biological effectiveness of very high gamma dose rate and its implication for radiological protection","volume":"59","author":"Olofsson","year":"2020","journal-title":"Radiat Environ Biophys."},{"key":"B58","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1080\/09553000601170469","article-title":"Kinetics and dose-response of residual 53BP1\/\u03b3-H2AX foci: Co-localization, relationship with DSB repair and clonogenic survival","volume":"83","author":"Markova","year":"2007","journal-title":"Int J Radiat Biol."},{"key":"B59","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.radonc.2011.05.065","article-title":"Assessing \u2018radiosensitivity'with kinetic profiles of \u03b3-H2AX, 53BP1 and BRCA1 foci","volume":"101","author":"Martin","year":"2011","journal-title":"Radiother Oncol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"696","DOI":"10.3109\/09553002.2010.549535","article-title":"Persistence of \u03b3-H2AX and 53BP1 foci in proliferating and non-proliferating human mammary epithelial cells after exposure to \u03b3-rays or iron ions","volume":"87","author":"Groesser","year":"2011","journal-title":"Int J Radiat Biol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-019-40339-6","article-title":"DNA DSB repair dynamics following irradiation with laser-driven protons at ultra-high dose rates","volume":"9","author":"Hanton","year":"2019","journal-title":"Sci Rep."},{"key":"B62","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.molcel.2008.05.017","article-title":"ATM signaling facilitates repair of DNA double-strand breaks associated with heterochromatin","volume":"31","author":"Goodarzi","year":"2008","journal-title":"Mol Cell"},{"key":"B63","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.freeradbiomed.2016.12.008","article-title":"Radiation-induced clustered DNA lesions: Repair and mutagenesis","volume":"107","author":"Sage","year":"2017","journal-title":"Free Radical Biol Med."},{"key":"B64","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.canlet.2012.01.007","article-title":"DNA damage-induced cell death: from specific DNA lesions to the DNA damage response and apoptosis","volume":"332","author":"Roos","year":"2013","journal-title":"Cancer Lett."},{"key":"B65","doi-asserted-by":"publisher","first-page":"519","DOI":"10.3390\/ijms19020519","article-title":"Live dynamics of 53BP1 foci following simultaneous induction of clustered and dispersed DNA damage in U2OS cells","volume":"19","author":"Sollazzo","year":"2018","journal-title":"Int J Mol Sci"},{"key":"B66","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1039\/C7NR06829H","article-title":"Particles with similar LET values generate DNA breaks of different complexity and reparability: a high-resolution microscopy analysis of \u03b3H2AX\/53BP1 foci","volume":"10","author":"Jezkova","year":"2018","journal-title":"Nanoscale."},{"key":"B67","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1667\/RR3123.1","article-title":"The non-homologous end-joining (NHEJ) pathway for the repair of DNA double-strand breaks: I. A mathematical model","volume":"179","author":"Taleei","year":"2013","journal-title":"Radiat Res."},{"key":"B68","doi-asserted-by":"publisher","first-page":"5470","DOI":"10.3390\/ijms22115470","article-title":"Evaluation of calyculin A effect on \u03b3H2AX\/53BP1 focus formation and apoptosis in human umbilical cord blood lymphocytes","volume":"22","author":"Zastko","year":"2021","journal-title":"Int J Mol Sci"},{"key":"B69","doi-asserted-by":"publisher","first-page":"389","DOI":"10.4161\/cc.9.2.10475","article-title":"The complexity of phosphorylated H2AX foci formation and DNA repair assembly at DNA double-strand breaks","volume":"9","author":"Nakamura","year":"2010","journal-title":"Cell cycle."},{"key":"B70","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.mrrev.2010.01.011","article-title":"Radiation-induced DNA repair foci: spatio-temporal aspects of formation, application for assessment of radiosensitivity and biological dosimetry","volume":"704","author":"Belyaev","year":"2010","journal-title":"Mutat Res."},{"key":"B71","doi-asserted-by":"publisher","first-page":"540575","DOI":"10.1101\/540575","article-title":"Concomitant chemotherapy increases radiotherapy-mediated DNA-damage in peripheral blood lymphocytes","volume":"2019","author":"Lorat","year":"2019","journal-title":"BioRxiv."},{"key":"B72","doi-asserted-by":"publisher","first-page":"653","DOI":"10.3109\/09553002.2015.1047987","article-title":"EPI-CT: in vitro assessment of the applicability of the \u03b3-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology","volume":"91","author":"Vandevoorde","year":"2015","journal-title":"Int J Radiat Biol."},{"key":"B73","article-title":"The Impact of Food Bioactives on Health: in vitro and ex vivo Models 388","author":"Verhoeckx","year":"2015"},{"key":"B74","doi-asserted-by":"publisher","first-page":"34","DOI":"10.11648\/j.ajlm.20160103.12","article-title":"Complete blood cell count and peripheral blood film, its significant in laboratory medicine: a review study","volume":"1","author":"Jacob","year":"2016","journal-title":"Am J Lab Med"},{"key":"B75","doi-asserted-by":"publisher","first-page":"90410P","DOI":"10.1117\/12.2043605","article-title":"Selection of the best features for leukocytes classification in blood smear microscopic images. In Medical Imaging 2014: Digital Pathology","volume":"9041","author":"Sarrafzadeh","year":"2014","journal-title":"Int Soc Opt Photon"},{"key":"B76","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-021-81058-1","article-title":"Comparison of DNA repair and radiosensitivity of different blood cell populations","volume":"11","author":"Heylmann","year":"2021","journal-title":"Sci Rep."},{"key":"B77","doi-asserted-by":"publisher","first-page":"2092","DOI":"10.4161\/cc.9.11.11810","article-title":"Dephosphorylation of \u03b3-H2AX by WIP1: an important homeostatic regulatory event in DNA repair and cell cycle control","volume":"9","author":"Moon","year":"2010","journal-title":"Cell cycle."},{"key":"B78","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/09553002.2022.1998706","article-title":"Establishment and validation of surface model for biodosimetry based on \u03b3-H2AX foci detection","volume":"2021","author":"L\u00f3pez","year":"2021","journal-title":"Int J Radiat Biol."},{"key":"B79","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.mrfmmm.2013.05.001","article-title":"Effect of blood storage conditions on DNA repair capacity measurements in peripheral blood mononuclear cells","volume":"749","author":"Allione","year":"2013","journal-title":"Mutat Res."},{"key":"B80","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1093\/mutage\/geab012","article-title":"Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies","volume":"36","author":"M\u00f8ller","year":"2021","journal-title":"Mutagenesis."}],"container-title":["Frontiers in Public Health"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpubh.2022.845200\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T22:40:11Z","timestamp":1676328011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpubh.2022.845200\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,8]]},"references-count":80,"alternative-id":["10.3389\/fpubh.2022.845200"],"URL":"https:\/\/doi.org\/10.3389\/fpubh.2022.845200","relation":{},"ISSN":["2296-2565"],"issn-type":[{"value":"2296-2565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,8]]},"article-number":"845200"}}