{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:33:17Z","timestamp":1779114797810,"version":"3.51.4"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Brucellosis is a zoonotic disease of mammals caused by bacterial species of the <jats:italic>Brucella<\/jats:italic> genus. The reservoir for disease is typically mammals, with species of <jats:italic>Brucella<\/jats:italic> found infecting amphibians, bats, and marine mammals. <jats:italic>Brucella<\/jats:italic> spp. can pass directly to humans through contact with infected animals or their products. <jats:italic>Brucella<\/jats:italic> spp. can cause chronic debilitating infections in mammals, including humans, and is associated with spontaneous abortions in infected animals, causing reduced fecundity. In Hawai\u2018i, terrestrial species that could harbor <jats:italic>Brucella<\/jats:italic> spp. include swine, cattle, horses, and axis deer among others. The numerous feral swine in Hawai\u2018i are known to carry <jats:italic>Brucella suis<\/jats:italic>, with evidence supporting infections in cattle. <jats:italic>Brucella suis<\/jats:italic> also poses infection risk to humans, dogs, and potentially horses across the state.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, 3,274 feral swine serum samples collected from 5 of the 8 main islands over a 15-year span were analyzed for exposure to <jats:italic>B. suis<\/jats:italic>. Of the 558 watersheds in the state, 77 were sampled as part of this effort. Spatial analysis was used to identify watersheds of concern. MLVA and whole genome SNP analysis was used for molecular epidemiological analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Statewide seropositivity rates were triple that of feral swine found in the conterminous United States. Smoothed positivity rates were highest on Maui, followed by O\u2018ahu, and the island of Hawai\u2018i. Island-by-island analysis found high brucellosis positivity levels associated with specific watersheds and agricultural areas. Local spatial autocorrelation identified hot spots on O\u2018ahu and Hawai\u2018i. MLVA analysis of available <jats:italic>B. suis<\/jats:italic> from Hawai\u2018i found molecular epidemiological connections with <jats:italic>B. suis<\/jats:italic> found in French Polynesia and the mainland US while differing from those in Tonga, Western Polynesia. Strains from Hawai\u2018i are phylogenetically closest to strains from the United States. MLVA and SNP analysis found <jats:italic>B. suis<\/jats:italic> strains from Hawai\u2018i fell into the genetic group that contains biovar 1 <jats:italic>B. suis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This work identified islands and watersheds of high brucellosis seropositivity in feral swine of Hawai\u2018i, highlighting the magnitude of the zoonotic risk. Introduction of strains in recent history is unlikely due to modern animal trade and disease control practices. Genomic analysis of strains in Hawai\u2018i and the Pacific area can provide hidden historical and local clues to brucellosis epidemiology in the state.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fpubh.2024.1440933","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T04:45:43Z","timestamp":1725425143000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Tracking sero-molecular trends of swine brucellosis in Hawai\u2018i and the central Pacific"],"prefix":"10.3389","volume":"12","author":[{"given":"Thi Hai Au","family":"La","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian A.","family":"McMillan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prashant","family":"Dahal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew H.","family":"Burger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Belcaid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darrin M.","family":"Phelps","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel M.","family":"Goldstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vienna R.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael H.","family":"Norris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1016\/j.ajpath.2015.03.003","article-title":"Pathogenesis and immunobiology of brucellosis: review of Brucella\u2013host interactions","volume":"185","author":"de Figueiredo","year":"2015","journal-title":"Am J Pathol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg Infect Dis."},{"key":"B3","unstructured":"CorbelMJ\n          Brucellosis in Humans and Animals2006"},{"key":"B4","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1093\/clinids\/21.2.283","article-title":"An overview of human brucellosis","volume":"21","author":"Young","year":"1995","journal-title":"Clin Infect Dis."},{"key":"B5","article-title":"Guide: exposures, testing, and prevention","volume-title":"Vol National Center for Emerging and Zoonotic Infectious Diseases Centers for Disease Control and Prevention.","author":"Brucellosis","year":"2017"},{"key":"B6","first-page":"523","article-title":"Interactions of the human pathogenic Brucella species with their hosts","volume-title":"Annual Review of Microbiology. 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