{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T08:07:38Z","timestamp":1758701258638,"version":"3.44.0"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:p>Wastewater-based epidemiology (WBE) provides a non-invasive, community-level approach to monitor infectious diseases such as COVID-19. This study investigated the temporal relationship between SARS-CoV-2 RNA levels in wastewater and reported COVID-19 cases in adjacent populations in Mexico City. A total of 40 samples were collected from the Copilco neighborhood during two epidemiological waves (April-September 2021 and November 2021-February 2022). An optimized one-step RT-qPCR protocol targeting the N1 gene achieved 96.7% efficiency with a detection limit of 10 copies\/\u03bcL. Spatial classification identified three proximity zones based on drainage system topology. Cross-correlation analysis between viral genome copies and confirmed case data revealed a significant temporal lag of 6-8 days. These results support the application of WBE as an early-warning tool to inform public health strategies and anticipate infection trends.<\/jats:p>","DOI":"10.3389\/fpubh.2025.1640581","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T05:35:10Z","timestamp":1755149710000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Temporal dynamics of SARS-CoV-2 detection in wastewater and population infection trends in Mexico City"],"prefix":"10.3389","volume":"13","author":[{"given":"Miguel Atl","family":"Silva-Maga\u00f1a","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisa","family":"Mazari-Hiriart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adalberto","family":"Noyola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana 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