{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:56:24Z","timestamp":1771930584676,"version":"3.50.1"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In December 2023, the local HIV confirmatory laboratory in a Southwestern city of China conducted molecular network analysis on newly reported cases of young students and nonstudent men who have sex with men (MSM) from 2018 to 2023 and identified a key molecular cluster. The aim of the study is to analyze the transmission network characteristics and elucidate the potential transmission dynamics within this cluster, and prevent further spread of the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cluster was designated as a key molecular cluster based on node characteristics, internal network density, and whether young students were involved. For the key cluster, a face-to-face epidemiological investigation was conducted, and detailed information on sociodemographic characteristics, histories of high-risk sexual behavior and sexual partners was collected. For those individuals identified through sexual partner tracing, HIV nucleic acid extraction, HIV-1\n                      <jats:italic>pol<\/jats:italic>\n                      gene amplification, and sequencing were performed. Molecular network and ML phylogenetic tree analyses were conducted to verify whether they belong to the same molecular cluster.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All 20 individuals in the key molecular cluster were male, with 9 being young students and 11 being nonstudent MSM The investigations revealed that three young students (A, B, C) provided the geosocial networking app (Blued) account information of the same high-risk sexual partner (H, was not included in the aforementioned molecular cluster), who actively solicited unprotected anal sex with these three individuals. H was contacted through Blued by local MSM organizations, and tested HIV-positive. After sexual partner tracing of H, he provided information on one high-risk sexual partner (D). Cross-referencing H\u2019s Blued data with local AIDS sexual partner tracing databases linked him to two young students diagnosed with HIV (E, F). Laboratory molecular network analysis confirmed that H belonged to the same molecular cluster as the aforementioned six people living with HIV (PLWH).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>By combining molecular transmission network analysis with epidemiological investigations, one individual with multiple high-risk sexual links who was initially not included in the molecular cluster was promptly detected, potentially preventing further transmission by creating awareness of HIV status and provision of HIV treatment services. It is necessary to improve the molecular network monitoring and conduct high-quality epidemiological investigation on the identified key molecular clusters as soon as possible to find persons who would benefit from HIV treatment and prevention services.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpubh.2026.1715356","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T06:49:05Z","timestamp":1770187745000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["An individual with multiple high-risk links uncovered: an epidemiological investigation based on molecular network analysis"],"prefix":"10.3389","volume":"14","author":[{"given":"Shijiao","family":"He","sequence":"first","affiliation":[{"name":"Department of Sexually Transmitted Diseases and HIV Prevention and Control, Chengdu Center for Disease Control and Prevention (Chengdu Institute of Health Supervision)","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ShanShan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of HIV Testing, Chengdu Center for Disease Control and Prevention (Chengdu Institute of Health Supervision)","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinying","family":"He","sequence":"additional","affiliation":[{"name":"Department of Sexually Transmitted Diseases and HIV Prevention and Control, Chengdu Center for Disease Control and Prevention (Chengdu Institute of Health Supervision)","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Sexually Transmitted Diseases and HIV Prevention and Control, Chengdu Center for Disease Control and Prevention (Chengdu Institute of Health 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Epidemiol"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1097\/qai.0000000000001695","article-title":"Leveraging phylogenetics to understand HIV transmission and partner notification networks","volume":"78","author":"Pasquale","year":"2018","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"e309","DOI":"10.1016\/S2352-3018(18)30062-6","article-title":"Non-disclosed men who have sex with men in UK HIV transmission networks: phylogenetic analysis of surveillance data","volume":"5","author":"Ragonnet-Cronin","year":"2018","journal-title":"Lancet HIV"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"712","DOI":"10.1089\/AID.2019.0293","article-title":"A geographic hotspot and emerging transmission cluster of the HIV-1 epidemic among older adults in a rural area of eastern China","volume":"36","author":"Jiang","year":"2020","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"e1006000","DOI":"10.1371\/journal.ppat.1006000","article-title":"Social and genetic networks of HIV-1 transmission in new York City","volume":"13","author":"Wertheim","year":"2017","journal-title":"PLoS Pathog"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1554","DOI":"10.3390\/pathogens10121554","article-title":"Epidemiology and molecular transmission characteristics of HIV in the capital city of Anhui Province in China","volume":"10","author":"Zheng","year":"2021","journal-title":"Pathogens"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1097\/QAD.0000000000001470","article-title":"Defining HIV-1 transmission clusters based on sequence data","volume":"31","author":"Hassan","year":"2017","journal-title":"AIDS"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"2875","DOI":"10.1097\/qad.0000000000001256","article-title":"Concurrency and HIV transmission network 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