{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:40:04Z","timestamp":1785584404671,"version":"3.56.0"},"reference-count":89,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012947","name":"Institut de Recherche pour le D\u00e9veloppement","doi-asserted-by":"publisher","award":["PE501078124-2022"],"award-info":[{"award-number":["PE501078124-2022"]}],"id":[{"id":"10.13039\/100012947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:p>\n                    Antibiotic-resistant bacteria, such as Extended-spectrum\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -lactamase-producing\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    ), represent a major threat to public health. Despite increasing reports of community-acquired ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    infections, the dynamics of ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    in low- and middle-income countries communities remains poorly understood. This study aimed to detect the fecal carriage of ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    at the human\u2013animal\u2013environment interface in Peru and to characterize underlying molecular mechanisms involved. We detected 15 ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    isolates among 652 (2.3%) fecal and water samples (i.e., 244 dairy cows from 25 farms, 261 pigs from 25 farms, 58 dogs, 39 farmers, and 50 water sources). ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    was isolated from two humans, two dogs, four cows, three pigs, and four water sources. Genomic analyses identified 10 different ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    sequence types (STs), including high-risk clones ST11, ST307, and ST37, as well as ST45, ST111, ST1, ST348, ST789, ST881, and ST983, and three CTX-M-encoding genes (\n                    <jats:italic>\n                      bla\n                      <jats:sub>CTX-M-15<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      bla\n                      <jats:sub>CTX-M-27<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      bla\n                      <jats:sub>CTX-M-14<\/jats:sub>\n                    <\/jats:italic>\n                    ). Four ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    STs (ST348, ST983, ST789, and ST11) were found in more than one source, both within and between farms. In particular, two ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    ST983 isolates, one from a human and one from a cow on the same farm, differed by 37 SNPs and had almost identical genetic profiles, suggesting bacterial clonal exchange across host species or contamination from a common source. Likewise, two nearly identical ST348 isolates were recovered from a cow and a water source on the same farm, suggesting ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    exchange between livestock and water. Our results highlight the circulation of ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    across humans, animals, and water in rural environments in Peru, representing one of the first genomic studies exploring potential cross-species ESBL-\n                    <jats:italic>Kp<\/jats:italic>\n                    relatedness in Latin America. Our study supports the need to implement sanitary measures to limit the cross-species spread of antimicrobial-resistant bacteria and to reduce water source contamination in Peru.\n                  <\/jats:p>","DOI":"10.3389\/fpubh.2026.1736106","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:44:09Z","timestamp":1770273849000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Circulation of ESBL-producing Klebsiella pneumoniae at the human\u2013animal\u2013environment interface in farms on the central coast of Peru"],"prefix":"10.3389","volume":"14","author":[{"given":"Julio A.","family":"Benavides","sequence":"first","affiliation":[{"name":"UMR MIVEGEC, IRD, CNRS, University of Montpellier","place":["Montpellier, France"]},{"name":"One Health Institute and Doctorado en Medicina de la Conservaci\u00f3n, Faculty of Life Sciences, Universidad Andr\u00e9s Bello","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00edlia","family":"Salgado-Caxito","sequence":"additional","affiliation":[{"name":"UMR MIVEGEC, IRD, CNRS, University of Montpellier","place":["Montpellier, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Escobar-P\u00e1ramo","sequence":"additional","affiliation":[{"name":"UMR MIVEGEC, IRD, CNRS, University of Montpellier","place":["Montpellier, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daphne","family":"L\u00e9on","sequence":"additional","affiliation":[{"name":"UMR MIVEGEC, IRD, CNRS, University of Montpellier","place":["Montpellier, France"]},{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis M.","family":"Jara","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Bardales-Mendoza","sequence":"additional","affiliation":[{"name":"Facultad de Educaci\u00f3n, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Murga","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Medrano","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]},{"name":"Emerge, Unidad de Investigaci\u00f3n en Enfermedades Emergentes y Cambio Clim\u00e1tico, Facultad de Salud P\u00fablica y Administraci\u00f3n, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Aylas-Jurado","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Su-Tello","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Najarro","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raiza","family":"Osorio-Linares","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional San Luis Gonzaga","place":["Ica, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u00edas","family":"Salvador-Tasayco","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional San Luis Gonzaga","place":["Ica, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Shiva","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia","place":["Lima, Peru"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1053\/jhin.2000.0862","article-title":"An outbreak of hospital-acquired Klebsiella pneumoniae bacteraemia, including strains producing extended-spectrum \u03b2-lactamase","volume":"47","author":"Pe\u00f1a","year":"2001","journal-title":"J Hosp Infect"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.meegid.2018.04.005","article-title":"Current epidemiology, genetic evolution and clinical impact of extended-spectrum \u03b2-lactamase-producing Escherichia coli and Klebsiella pneumoniae","volume":"61","author":"Chong","year":"2018","journal-title":"Infect Genet Evol"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1186\/s12941-023-00638-3","article-title":"\u201cOne health\u201d perspective on prevalence of co-existing 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